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Safety profiles of beneficial lactic acid bacteria isolated from dairy systems

  • Food Microbiology - Research Paper
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Abstract

This study aimed to assess the safety aspects of 15 lactic acid bacteria (LAB) strains previously isolated from a dairy environment with relation to their beneficial features. LAB strains were assessed using phenotypic methods according to their production of virulence factors at 25 °C and 37 °C, as well as by examining their potential resistance to 15 antibiotics. Polymerase chain reaction (PCR) was also used to identify the presence of 50 genes associated with virulence factors and antibiotic resistance in the strains. None of the strains presented hemolytic activity or the production of gelatinase, lipase, deoxyribonuclease, or the tested biogenic amines. Based on the disk diffusion assay, all strains were resistant to oxacillin and sulfa/trimethoprim. Further, some were resistant to gentamicin (14), clindamycin (11), vancomycin (9), rifampicin (8), erythromycin (5), tetracycline (4), ampicillin (2), and chloramphenicol (1); no strain was resistant to imipenem. Regarding virulence- and antibiotic-resistance-related genes, 19 out of 50 tested genes were present in some strains; there was a variable association of expression. Based on the obtained data, the isolates presented relatively safe characteristics and behavior, findings that should lead to further studies to assess their potential usage as beneficial cultures in the food industry.

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Funding

This study was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasília, DF, Brazil—financial code 001), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasília, DF, Brazil), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, Belo Horizonte, MG, Brazil).

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Authors and Affiliations

  1. Departamento de Veterinária, InsPOA - Laboratório de Inspeção de Produtos de Origem Animal, Universidade Federal de Viçosa, Campus Viçosa s/n - Centro, Viçosa, MG, 36570-900, Brazil

    Monique Colombo, Luis Augusto Nero & Svetoslav Dimitrov Todorov

  2. Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Laboratório de Microbiologia de Alimentos, Universidade de São Paulo, Av. Prof. Lineu Prestes 580 Bloco 14, São Paulo, SP, 05508-900, Brazil

    Svetoslav Dimitrov Todorov

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  1. Monique Colombo
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  2. Luis Augusto Nero
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  3. Svetoslav Dimitrov Todorov
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Correspondence to Luis Augusto Nero or Svetoslav Dimitrov Todorov.

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Responsible Editor: Elaine Cristina Pereira de Martinis.

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This manuscript was organized based on results obtained by the first author during her Doctorate training, and fully described in her thesis [16].

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Highlights

• The safety aspects of LAB isolated from a dairy environment was evaluated.

• Presence of 49 virulence factors and antibiotic resistance genes was studied.

• Physiological expression of virulence factors, biogenic amine, and antibiotic resistance was tested.

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Colombo, M., Nero, L.A. & Todorov, S.D. Safety profiles of beneficial lactic acid bacteria isolated from dairy systems. Braz J Microbiol 51, 787–795 (2020). https://doi.org/10.1007/s42770-020-00227-y

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