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A Whole-brain Map of Long-range Inputs to GABAergic Interneurons in the Mouse Caudal Forelimb Area

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Abstract

The caudal forelimb area (CFA) of the mouse cortex is essential in many forelimb movements, and diverse types of GABAergic interneuron in the CFA are distinct in the mediation of cortical inhibition in motor information processing. However, their long-range inputs remain unclear. In the present study, we combined the monosynaptic rabies virus system with Cre driver mouse lines to generate a whole-brain map of the inputs to three major inhibitory interneuron types in the CFA. We discovered that each type was innervated by the same upstream areas, but there were quantitative differences in the inputs from the cortex, thalamus, and pallidum. Comparing the locations of the interneurons in two sub-regions of the CFA, we discovered that their long-range inputs were remarkably different in distribution and proportion. This whole-brain mapping indicates the existence of parallel pathway organization in the forelimb subnetwork and provides insight into the inhibitory processes in forelimb movement to reveal the structural architecture underlying the functions of the CFA.

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Acknowledgements

We thank Qingtao Sun, Pan Luo, Mei Yao, and Peilin Zhao for help with experiments and data analysis. We thank the Optical Bioimaging Core Facility of Huazhong University of Science and Technology for providing support with data acquisition. This work was supported by the National Natural Science Foundation of China (61721092, 91749209, and 31871088) and the Director Fund of Wuhan National Laboratory for Optoelectronics.

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Authors and Affiliations

  1. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics–Huazhong University of Science and Technology, Wuhan, 430074, China

    Zhuonan Duan, Anan Li, Hui Gong & Xiangning Li

  2. Ministry of Education Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, 430074, China

    Zhuonan Duan, Anan Li, Hui Gong & Xiangning Li

  3. Huazhong University of Science and Technology–Suzhou Institute for Brainsmatics, Suzhou, 215125, China

    Anan Li, Hui Gong & Xiangning Li

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  1. Zhuonan Duan
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Correspondence to Xiangning Li.

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Duan, Z., Li, A., Gong, H. et al. A Whole-brain Map of Long-range Inputs to GABAergic Interneurons in the Mouse Caudal Forelimb Area. Neurosci. Bull. 36, 493–505 (2020). https://doi.org/10.1007/s12264-019-00458-6

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  • DOI: https://doi.org/10.1007/s12264-019-00458-6

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