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Up-regulation of miR-106a targets LIMK1 and contributes to cognitive impairment induced by isoflurane anesthesia in mice

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Abstract

Background

Postoperative cognitive dysfunction (POCD) had a great relationship with anesthesia during surgery, and miRNAs have been found involved in anesthesia-induced cognitive impairment.

Objective

To explore the role and potential mechanism of miR-106a in isoflurane anesthesia-induced cognitive impairment.

Methods

Adult male mice were treated with isoflurane anesthesia; Morris water maze tests and fear conditioning tests were performed; and expression levels of miR-106a and LIMK1 were determined by quantitative real-time PCR (qRT-PCR) and western blot. Dual luciferase reporter assay was used to determine the binding of miR-106a and 3’UTR of LIMK1. To verify the role of miR-106a, antagomir of miR-106a were intrahippocampally injected. Finally, expression of BCL2 apoptosis regulator (Bcl-2), LIM domain kinase 1 (LIMK1), BCL2-associated X, apoptosis regulator (Bax) and cleaved caspase3 was determined by western blot.

Results

In isoflurane anesthesia-treated group (IS), the percentage of target quadrant dwell time was significantly lower and the escape latency was significantly higher than in the control group (sham), and the freezing behavior of IS was significantly less in contextual fear conditioning tests. Expression levels of miR-106a were increased and those of LIMK1 were decreased in response to IS. Dual luciferase reporter assay showed that miR-106a could bind with the 3’UTR of LIMK1. Decreased expression levels of miR-106a improved the cognitive impairment of the mice treated with isoflurane. Intrahippocampally injected antagomir of miR-106a also increased LIMK1 and Bcl-2 levels, decreased the BAX and cleaved caspase3 expression levels in the mice treated with isoflurane.

Conclusion

Decrease of LIMK1 expression by miR-106a played an important role in isoflurane anesthesia-induced cognitive impairment.

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All data generated or analyzed during this study are included in this published article.

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Authors and Affiliations

  1. Department of Anesthesia, Peking University International Hospital, No. 1, Life Garden Road, Zhongguancun Life Garden, Changping District, Beijing, China

    Ning Zhang, Hui Wen & Lan Yao

  2. Department of Anesthesia, Xuanwu Hospital of Capital Medical University, Beijing, 100053, China

    Weiguang Ye & Tianlong Wang

Authors
  1. Ning Zhang
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  2. Weiguang Ye
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  3. Tianlong Wang
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  4. Hui Wen
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  5. Lan Yao
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Contributions

NZ and LY conceived and designed the experiments, WGY analyzed and interpreted the results of the experiments, TLW and HW performed the experiments.

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Correspondence to Lan Yao.

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The animal use peotocol listed below has been reviewde and approved by the Animal Ethical and Welfaer Committee.

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Zhang, N., Ye, W., Wang, T. et al. Up-regulation of miR-106a targets LIMK1 and contributes to cognitive impairment induced by isoflurane anesthesia in mice. Genes Genom 42, 405–412 (2020). https://doi.org/10.1007/s13258-019-00913-8

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  • DOI: https://doi.org/10.1007/s13258-019-00913-8

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