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Endoreduplication in Drosophila melanogaster progeny after exposure to acute γ-irradiation

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Abstract

The purpose of this investigation was to study the effect of acute γ-irradiation of parent adults on the endoreduplication of giant chromosomes in F1 generation of Drosophila melanogaster Meig. A wild-type Oregon-R strain was used as the material. Virgin females and males of Drosophila adults at the age of 3 days were irradiated with doses of 8, 16 and 25 Gy. Giant chromosomes were studied by cytomorphometry on squashed preparations of Drosophila salivary glands stained with acetoorsein. The preparations were obtained at late third instar larvae. The mean values of the polyteny degree of chromosomes (PDC) in males increased after 8 Gy by 10.6%, after 25 Gy by 7.4%, and did not change after the dose of 16 Gy. In females, the PDC did not differ from the control irrespective of the irradiation dose. An increase in endoreduplication was also evidenced by the accelerated development of offsprings of both sexes after irradiation of parents with 25 Gy, and in males also at a dose of 16 Gy. The statistical impact of power of radiation on polyteny was 26.8%, while the impact of sex was 4.9%. The impact of power of radiation on the developmental rate of offspring was 4.4% in males and 7.5% in females. The enhancement of endoreduplication is considered as a consequence of increasing selection pressure after irradiation. The possible involvement of epigenetic effects in the effect of ionizing radiation on endoreduplication is discussed.

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Acknowledgements

This work was supported by the Ministry of Education and Science of Ukraine (Project State registration number: 0117U004836).

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  1. Department of Genetics and Cytology, VN Karazin Kharkiv National University, Svobody sq., 4, Kharkiv, 61022, Ukraine

    Daria A. Skorobagatko & Volodymyr Yu. Strashnyuk

  2. Laboratory of Radiation Research and Environmental Protection, NSC ‘Kharkiv Institute of Physics and Technology’, Academicheskaya str., 1, Kharkiv, 61108, Ukraine

    Daria A. Skorobagatko & Alexey A. Mazilov

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  1. Daria A. Skorobagatko
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Skorobagatko, D.A., Mazilov, A.A. & Strashnyuk, V.Y. Endoreduplication in Drosophila melanogaster progeny after exposure to acute γ-irradiation. Radiat Environ Biophys 59, 211–220 (2020). https://doi.org/10.1007/s00411-019-00828-8

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