Abstract
Pentachlorophenol (PCP) is a widespread and persistent hydrophobic organic pollutant in the environment despite its restricted public use. Risk assessment of such hydrophobic organic compounds (HOCs) is challenging because sorption and volatilization issues during toxicity test often lead to inconsistent exposure concentration. Considering the hydrophobicity of the PCP, in this study, a passive dosing format was applied by adopting a silicone O-ring as a reservoir and evaluated its applicability on the determination of PCP on Daphnia magna. Results obtained with passive dosing method were compared with that of solvent spiking method. We hypothesized that the passive dosing method may provide more reliable and accurate toxicity results than conventional solvent spiking approach. As a result, the partition coefficient of PCP between methanol and a test medium (log KMeOH:ISO) was 2.1, which enabled the maintenance of reliable exposure concentration throughout the experiment. In the acute toxicity tests, passive dosing and solvent spiking showed similar EC50 values of 576 and 485 µg/L for 24 h, and 362 and 374 µg/L for 48 h, respectively, which overlap with EC50 values of previous studies. Altogether, both methods were suitable for the acute toxicity assessment of hydrophobic PCP.
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References
Agbo SO, Küster E, Georgi A et al. (2011) Photostability and toxicity of pentachlorophenol and phenanthrene. J Hazard Mater 189:235–240. https://doi.org/10.1016/j.jhazmat.2011.02.024
ATSDR (2001) Toxicological profile for Pentachlorophenol. Agency for toxic substances and disease registry. U.S. Department of Health and Human Services, Public Health Service, Atlanta, GA. https://www.atsdr.cdc.gov/ToxProfiles/tp51.pdf
Baun A, Sørensen SN, Rasmussen RF et al. (2008) Toxicity and bioaccumulation of xenobiotic organic compounds in the presence of aqueous suspensions of aggregates of nano-C60. Aquat Toxicol 86:379–387. https://doi.org/10.1016/j.aquatox.2007.11.019
Berglind R, Dave G (1984) Acute toxicity of chromate, DDT, POP, TPBS, and zinc to Daphnia magna cultured in hard and soft water. Bull Environ Contam Toxicol 33:63–68
Bettinetti R, Kopp-Schneider A, Vignati DAL (2018) The European water-based environmental quality standard for pentachlorophenol is NOT protective of benthic organisms. Sci Total Environ 613–614:39–45. https://doi.org/10.1016/j.scitotenv.2017.09.055
Birch H, Gouliarmou V, Lützhoft HCH et al. (2010) Passive dosing to determine the speciation of hydrophobic organic chemicals in aqueous samples. Anal Chem 82:1142–1146. https://doi.org/10.1021/ac902378w
Birch H, Hammershøj R, Mayer P (2018) Determining biodegradation kinetics of hydrocarbons at low concentrations: covering 5 and 9 orders of magnitude of kowand kaw. Environ Sci Technol 52:2143–2151. https://doi.org/10.1021/acs.est.7b05624
Bjergager MBA, Dalhoff K, Kretschmann A et al. (2017) Determining lower threshold concentrations for synergistic effects. Aquat Toxicol 182:79–90. https://doi.org/10.1016/j.aquatox.2016.10.020
Bragin GE, Parkerton TF, Redman AD et al. (2016) Chronic toxicity of selected polycyclic aromatic hydrocarbons to algae and crustaceans using passive dosing. Environ Toxicol Chem 35:2948–2957. https://doi.org/10.1002/etc.3479
Butler JD, Parkerton TF, Letinski DJ et al. (2013) A novel passive dosing system for determining the toxicity of phenanthrene to early life stages of zebrafish. Sci Total Environ 463–464:952–958. https://doi.org/10.1016/j.scitotenv.2013.06.079
Calleja MC, Persoone G (1993) The influence of solvents on the acute toxicity of some lipophilic chemicals to aquatic invertebrates. Chemosphere 26:2007–2022. https://doi.org/10.1016/S0957-4166(97)00059-1
EC (2009) Commission regulation (EU) No 207/2011 of 2 Mar 2011 amending regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) as regards Annex XVII. Off J Eur Union. https://doi.org/10.1016/S1351-4210(11)70151-2
Ellgehausen H, Guth JA, Esser HO (1980) Factors determining the bioaccumulation potential of pesticides in the individual compartments of aquatic food chains. Ecotoxicol Environ Saf 4:134–157. https://doi.org/10.1016/0147-6513(80)90015-9
Fischer F, Böhm L, Höss S et al. (2016) Passive dosing in chronic toxicity tests with the nematode caenorhabditis elegans. Environ Sci Technol 50:9708–9716. https://doi.org/10.1021/acs.est.6b02956
Gilroy ÈAM, Muir DCG, McMaster ME et al. (2017) Halogenated phenolic compounds in wild fish from Canadian areas of concern. Environ Toxicol Chem 36:2266–2273. https://doi.org/10.1002/etc.3781
Hickey CW (1989) Sensitivity of four new zealand cladoceran species and Daphnia magna to aquatic toxicants. N Zeal J Mar Freshw Res 23:131–137. https://doi.org/10.1080/00288330.1989.9516348
Hickey CW, Vickers ML (1992) Comparison of the sensitivity to heavy metals and pentachlorophenol of the mayflies deleatidium spp. and the cladoceran Daphnia magna. N. Zeal J Mar Freshw Res 26:87–93. https://doi.org/10.1080/00288330.1992.9516504
Hutchinson TH, Shillabeer N, Winter MJ, Pickford DB (2006) Acute and chronic effects of carrier solvents in aquatic organisms: a critical review. Aquat Toxicol 76:69–92. https://doi.org/10.1016/j.aquatox.2005.09.008
Ikuno E, Matsumoto T, Okubo T et al. (2008) Difference in the sensitivity to chemical compounds between female and male neonates of Daphnia magna. Environ Toxicol 23:570–575. https://doi.org/10.1002/tox
Jugan ML, Lévy-Bimbot M, Pomérance M et al. (2007) A new bioluminescent cellular assay to measure the transcriptional effects of chemicals that modulate the alpha-1 thyroid hormone receptor. Toxicol In Vitro 21:1197–1205. https://doi.org/10.1016/j.tiv.2007.03.020
Kramer NI, Busser FJM, Oosterwijk MTT et al. (2010) Development of a partition-controlled dosing system for cell assays. Chem Res Toxicol 23:1806–1814. https://doi.org/10.1021/tx1002595
Kwon JH, Wuethrich T, Mayer P, Escher BI (2007) Dynamic permeation method to determine partition coefficients of highly hydrophobic chemicals between poly(dimethylsiloxane) and water. Anal Chem 79:6816–6822. https://doi.org/10.1021/ac0710073
Lee SK, Freitag D, Steinberg C et al. (1993) Effects of dissolved humic materials on acute toxicity of some organic chemicals to aquatic organisms. Water Res 27:199–204
Levin H, Hahn J (1986) Pentachlorophenol in indoor air: methods to reduce airborne concentrations. Environ Int 12:333–341. https://doi.org/10.1016/0160-4120(86)90047-4
Lombardi JV, Bazente-Yamaguishi R, Madeira FF et al. (2018) Potassium chloride and sodium chloride as reference toxicants to assess quality of toxicity tests carried out with the microcrustacean cladocera Ceriodaphnia dubia. Bol Inst Pesca 44:e311. https://doi.org/10.20950/1678-2305.2018.311V
Mathialagan T, Viraraghavan T (2005) Biosorption of pentachlorophenol by fungal biomass from aqueous solutions: a factorial design analysis. Environ Technol 26:571–579. https://doi.org/10.1080/09593332608618542
Mayer P, Wernsing J, Tolls J et al. (1999) Establishing and controlling dissolved concentrations of hydrophobic organics by partitioning from a solid phase. Environ Sci Technol 33:2284–2290. https://doi.org/10.1021/es9808898
McCarthy JF (1983) Role of particulate organic matter in decreasing by Daphnia magna. Arch Environ Contam Toxicol 12:559–568. https://doi.org/10.1007/BF01056552
McConnell EE, Huff JE, Hejtmancik M et al. (1991) Toxicology and carcinogenesis studies of two grades of pentachlorophenol in B6C3F1 mice. Toxicol Sci 17:519–532. https://doi.org/10.1093/toxsci/17.3.519
Niehus NC, Floeter C, Hollert H, Witt G (2018) Miniaturised marine algae test with polycyclic aromatic hydrocarbons − comparing equilibrium passive dosing and nominal spiking. Aquat Toxicol 198:190–197. https://doi.org/10.1016/j.aquatox.2018.03.002
Oda S, Tatarazako N, Watanabe H et al. (2006) Genetic differences in the production of male neonates in Daphnia magna exposed to juvenile hormone analogs. Chemosphere 63:1477–1484. https://doi.org/10.1016/j.chemosphere.2005.09.026
OECD (2004) OECD guidelines for testing of chemicals. Daphnia sp., Acute Immobilisation Test no. 202. https://doi.org/10.1787/9789264069947-en
OECD (2012) OECD guideline for the testing of chemicals. Daphnia magna Reproduction Test no. 211. https://doi.org/10.1787/9789264185203-en
Orvos DR, Versteeg DJ, Inauen J et al. (2002) Aquatic toxicity of triclosan. Environ Toxicol Chem 21:1338–1349. https://doi.org/10.1007/s11045-016-0420-5
Parks LG, LeBlanc GA (1996) Reductions in steroid hormone biotransformation/elimination as a biomarker of pentachlorophenol chronic toxicity. Aquat Toxicol 34:291–303. https://doi.org/10.1016/0166-445X(95)00045-6
Rawlings NC, Cook SJ, Waldbillig D (1998) Effects of the pesticides carbofuran, chlorpyrifos, dimethoate, lindane, triallate, trifluralin, 2,4-D, andpentachlorophenol on the metabolic endocrine and reproductive endocrine system in ewes. J Toxicol Environ Heal 54:21–36. https://doi.org/10.1080/009841098159006
Repetto G, Jos A, Molero ML (2001) A test battery for the ecotoxicological evaluation of the pentachlorophenol. Toxicol In Vitro 15:503–509. https://doi.org/10.1016/j.ecoenv.2004.01.001
Ribbenstedt A, Mustajärvi L, Breitholtz M et al. (2017) Passive dosing of triclosan in multigeneration tests with copepods—stable exposure concentrations and effects at the low µg/L range. Environ Toxicol Chem 36:1254–1260. https://doi.org/10.1002/etc.3649
Sánchez P, Alonso C, Fernández C et al. (2005) Evaluation of a multi-species test system for assessing acute and chronic toxicity of sediments and water to aquatic invertebrates effects of pentachlorophenol on Daphnia magna and Chironomus prasinus. J Soils Sediment 5:53–58. https://doi.org/10.1065/jss2004.10.114
Smith KEC, Dom N, Blust R, Mayer P (2010a) Controlling and maintaining exposure of hydrophobic organic compounds in aquatic toxicity tests by passive dosing. Aquat Toxicol 98:15–24. https://doi.org/10.1016/j.aquatox.2010.01.007
Smith KEC, Heringa MB, Uytewaal M, Mayer P (2013) The dosing determines mutagenicity of hydrophobic compounds in the Ames II assay with metabolic transformation: passive dosing versus solvent spiking. Mutat Res 750:12–18. https://doi.org/10.1016/j.mrgentox.2012.07.006
Smith KEC, Jeong Y, Kim J (2015) Passive dosing versus solvent spiking for controlling and maintaining hydrophobic organic compound exposure in the Microtox® assay. Chemosphere 139:174–180. https://doi.org/10.1016/j.chemosphere.2015.06.028
Smith KEC, Oostingh GJ, Mayer P (2010b) Passive dosing for producing defined and constant exposure of hydrophobic organic compounds during in vitro toxicity tests. Chem Res Toxicol 23:55–65. https://doi.org/10.1021/tx900274j
Sprunger L, Proctor A, Acree WE, Abraham MH (2007) Characterization of the sorption of gaseous and organic solutes onto polydimethyl siloxane solid-phase microextraction surfaces using the Abraham model. J Chromatogr A 1175:162–173. https://doi.org/10.1016/j.chroma.2007.10.058
Stibany F, Ewald F, Miller I et al. (2017a) Improving the reliability of aquatic toxicity testing of hydrophobic chemicals via equilibrium passive dosing—a multiple trophic level case study on bromochlorophene. Sci Total Environ 584–585:96–104. https://doi.org/10.1016/j.scitotenv.2017.01.082
Stibany F, Schmidt SN, Schäffer A, Mayer P (2017b) Aquatic toxicity testing of liquid hydrophobic chemicals—passive dosing exactly at the saturation limit. Chemosphere 167:551–558. https://doi.org/10.1016/j.chemosphere.2018.07.150
Trac LN, Schmidt SN, Mayer P (2018) Headspace passive dosing of volatile hydrophobic chemicals—aquatic toxicity testing exactly at the saturation level. Chemosphere 211:694–700. https://doi.org/10.1016/j.chemosphere.2018.07.150
Tran DQ, Klotz DM, Ladlie BL et al. (1996) Inhibition of progesterone receptor activity in yeast by synthetic chemicals. Biochem Biophys Res Commun 229:518–523. https://doi.org/10.1006/bbrc.1996.1836
Vergauwen L, Schmidt SN, Stinckens E et al. (2015) A high throughput passive dosing format for the Fish Embryo Acute Toxicity test. Chemosphere 139:9–17. https://doi.org/10.1016/j.chemosphere.2015.05.041
Vulliet E, Tournier M, Vauchez A et al. (2014) Survey regarding the occurrence of selected organic micropollutants in the groundwaters of overseas departments. Environ Sci Pollut Res 21:7512–7521. https://doi.org/10.1007/s11356-014-2619-z
Weschler CJ (2009) Changes in indoor pollutants since the 1950s. Atmos Environ 43:153–169. https://doi.org/10.1016/j.atmosenv.2008.09.044
Xia X, Li H, Yang Z et al. (2015) How does predation affect the bioaccumulation of hydrophobic organic compounds in aquatic organisms? Environ Sci Technol 49:4911–4920. https://doi.org/10.1021/acs.est.5b00071
Xing L, Liu H, Giesy JP, Yu H (2012) pH-dependent aquatic criteria for 2,4-dichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol. Sci Total Environ 441:125–131. https://doi.org/10.1016/j.scitotenv.2012.09.060
Zhang X, Xia X, Li H et al. (2015) Bioavailability of pyrene associated with suspended sediment of different grain sizes to Daphnia magna as investigated by passive dosing devices. Environ Sci Technol 49:10127–10135. https://doi.org/10.1021/acs.est.5b02045
Zheng W, Yu H, Wang X, Qu W (2012) Systematic review of pentachlorophenol occurrence in the environment and in humans in China: not a negligible health risk due to the re-emergence of schistosomiasis. Environ Int 42:105–116. https://doi.org/10.1016/j.envint.2011.04.014
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This work was supported by the internal project of Korea Institute of Science and Technology Europe (No. 11896), and Kyungsung University Research Grants in 2019.
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Kwon, Ha., Jeong, Y., Jeon, H.P. et al. Comparing passive dosing and solvent spiking methods to determine the acute toxic effect of pentachlorophenol on Daphnia magna. Ecotoxicology 29, 286–294 (2020). https://doi.org/10.1007/s10646-020-02172-w
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DOI: https://doi.org/10.1007/s10646-020-02172-w