Key message
Fine mapping of the barley leaf rust resistance locus Rph13 on chromosome 3HL facilitates its use in breeding programs through marker-assisted selection.
Abstract
Barley leaf rust (BLR—caused by Puccinia hordei) is a widespread fungal disease that can be effectively controlled by genetic resistance. There is an ongoing need to both diversify and genetically characterise resistance loci to provide effective and durable control given the ongoing threat of rapidly evolving P. hordei populations. Here, we report on the molecular genetic characterisation of the Rph13 locus, originally derived from wild barley and transferred to barley accession Berac (then referred to as PI 531849). The 2017 reference genome of cv. Morex was used as a road map to rapidly narrow both a genetic and physical intervals around the Rph13 resistance locus. Using recombination-based mapping, we narrowed the physical interval to 116.6 kb on chromosome 3H in a segregating population of a cross of the Rph13 carrying resistant line PI 531849 with the leaf rust-susceptible cultivar Gus. We identified two nucleotide-binding leucine-rich repeat genes as likely candidates for the Rph13 resistance. Sequences from the candidate genes enabled the development of a KASP marker that distinguished resistant and susceptible progeny and was found to be predictive and useful for MAS.
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Acknowledgements
The authors would like to thank Ms Bethany Clark, Mr Matthew Williams and Mr Gary Standen for technical assistance and the Grains Research Development Corporation for funding this project (US00074).
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MJ and PD designed and performed all experiments, analysed and interpreted results. DS contributed plant material for the study. MJ and PD wrote the manuscript. All authors reviewed and approved the manuscript. RP and EL conceptualised the research and received funding.
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Communicated by Albrecht E. Melchinger.
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Jost, M., Singh, D., Lagudah, E. et al. Fine mapping of leaf rust resistance gene Rph13 from wild barley. Theor Appl Genet 133, 1887–1895 (2020). https://doi.org/10.1007/s00122-020-03564-6
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DOI: https://doi.org/10.1007/s00122-020-03564-6