Peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) for bisulfite-free quantification of locus-specific 5-hydroxymethylcytosine

Zhenhao Zhang; Jingli Yan; Zhengping Li

doi:10.1039/D0CC00167H

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Peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) for bisulfite-free quantification of locus-specific 5-hydroxymethylcytosine†

Zhenhao Zhang,^a Jingli Yan*^a and Zhengping Li

*^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, Shaanxi Province, P. R. China
E-mail: lzpbd@snnu.edu.cn, yanjingli@snnu.edu.cn

Abstract

The peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) is applied for the first time to the sensitive detection of locus-specific 5-hydroxymethylcytosine (5hmC) in DNA with low non-specific amplification. This strategy greatly shortens reaction time, improves sensitivity, and reduces non-specific amplification.

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Article information

DOI: https://doi.org/10.1039/D0CC00167H
Article type: Communication
Submitted: 08 Jan 2020
Accepted: 18 Feb 2020
First published: 18 Feb 2020

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Chem. Commun., 2020,56, 3111-3114

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Peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) for bisulfite-free quantification of locus-specific 5-hydroxymethylcytosine

Z. Zhang, J. Yan and Z. Li, Chem. Commun., 2020, 56, 3111 DOI: 10.1039/D0CC00167H

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Chemical Communications

Peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) for bisulfite-free quantification of locus-specific 5-hydroxymethylcytosine†

Abstract

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Article information

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Peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) for bisulfite-free quantification of locus-specific 5-hydroxymethylcytosine

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