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Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in large-volume samples

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Abstract

To separate and concentrate koi herpesvirus (KHV) from large-volume samples, a separation method based on immunomagnetic beads (IMBs) coated with polyclonal antibody directed against KHV was developed. After treatment with IMBs, viral DNA was extracted from samples and used as a template for quantitative PCR (qPCR). The results showed that the concentration of the template DNA extracted from the virus that had been separated using IMBs was 9.65-fold higher than that from virus not treated with IMBs. The detection limit of the IMBs/qPCR method was found to be at least 10 times lower than that of qPCR alone.

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Acknowledgements

This study was supported by the Scientific Research Foundation of the Chinese Academy of Inspection and Quarantine (CAIQ) (2018JK011).

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Authors and Affiliations

  1. The Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, No. 11 Building, Ronghua South Road, Daxing District, Beijing, 100176, China

    Min Zhang, Hongli Jing, Na Wang, Shaoqiang Wu & Xiangmei Lin

  2. Key Laboratory of Fishery Drug Development of the Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China

    Yingying Li & Qing Wang

Authors
  1. Min Zhang
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  2. Yingying Li
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  3. Hongli Jing
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  4. Na Wang
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  5. Shaoqiang Wu
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  6. Qing Wang
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  7. Xiangmei Lin
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Correspondence to Qing Wang or Xiangmei Lin.

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Zhang, M., Li, Y., Jing, H. et al. Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in large-volume samples. Arch Virol 165, 973–976 (2020). https://doi.org/10.1007/s00705-020-04557-y

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  • DOI: https://doi.org/10.1007/s00705-020-04557-y

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