Abstract
Peptide-amphiphile (PA)-based supramolecular thixotropic hydrogels are useful in medical sciences due to multiple advantageous features along with its biocompatibility and biodegradability. In this work, we have developed a self-assembled peptide-based hydrogel from a \(\upbeta \)-sheet forming short PA. Hydrogelation of the PA is controlled by pH and consequent changes in secondary structures attained by the PA. Under acidic conditions, the PA remains in random coil conformation. While increasing the pH to 9, a rapid transformation to anti-parallel \(\upbeta \)-sheet leads to a strong hydrogel. Interestingly, the disulphide-linked dimer of the PA failed to attain such aggregation pattern. The pH-induced sol–gel–sol transition can be achieved for several cycles without any change in aggregation pattern. The hydrogel was also found to show thixotropic rheological behaviour and thus, it can be utilized as an injectable hydrogel for biomedical applications.
Similar content being viewed by others
References
Gong Z, Yang Y, Ren Q, Chen X and Shao Z 2012 Soft Matter 8 2875
Pramanik B, Ahmed S, Singha N, Das B K, Dowari P and Das D 2019 Langmuir 35 478
Hamilton T D, Bučar D-K, Baltrusaitis J, Flanagan D R, Li Y, Ghorai S et al 2011 J. Am. Chem. Soc. 133 3365
Lee J H 2018 Biomater. Res. 22 27
Mathew A P, Uthaman S, Cho K-H, Cho C-S and Park I-K 2018 Int. J. Biol. Macromol. 110 17
Dasgupta A, Mondal J H and Das D 2013 RSC Adv. 3 9117
Xing R, Li S, Zhang N, Shen G, Möhwald H and Yan X 2017 Biomacromolecules 18 3514
Kumar D, Workman V L, O’Brien M, McLaren J, White L, Ragunath K et al 2017 Adv. Funct. Mater. 27 1702424
Collier J H, Rudra J S, Gasiorowski J Z and Jung J P 2010 Chem. Soc. Rev. 39 3413
Altunbas A and Pochan D J 2012 in Peptide-based materials T Deming (ed) (Heidelberg, Berlin: Springer) p 135
Nagai Y, Unsworth L D, Koutsopoulos S and Zhang S 2006 J. Controlled Release 115 18
Singha N, Srivastava A, Pramanik B, Ahmed S, Dowari P, Chowdhuri S et al 2019 Chem. Sci. 10 5920
Ahmed S, Mondal J H, Behera N and Das D 2013 Langmuir 29 14274
Mitra R N and Das P K 2008 J. Phys. Chem. C 112 8159
Pramanik B, Singha N and Das D 2019 ACS Appl. Polym. Mater. 1 833
Singha N, Neogi S, Pramanik B, Das S, Dasgupta A, Ghosh R et al 2019 ACS Appl. Polym. Mater. 1 2267
Li J, Xing R, Bai S and Yan X 2019 Soft Matter 15 1704
Cui H, Webber M J and Stupp S I 2010 Pep. Sci. 94 1
Pochan D J, Schneider J P, Kretsinger J, Ozbas B, Rajagopal K and Haines L 2003 J. Am. Chem. Soc. 125 11802
Haines L A, Rajagopal K, Ozbas B, Salick D A, Pochan D J and Schneider J P 2005 J. Am. Chem. Soc. 127 17025
Kretsinger J K, Haines L A, Ozbas B, Pochan D J and Schneider J P 2005 Biomaterials 26 5177
Haines-Butterick L, Rajagopal K, Branco M, Salick D, Rughani R, Pilarz M et al 2007 Proc. Natl. Acad. Sci. 104 7791
Rughani R V, Salick D A, Lamm M S, Yucel T, Pochan D J and Schneider J P 2009 Biomacromolecules 10 1295
Nagarkar R P, Hule R A, Pochan D J and Schneider J P 2008 J. Am. Chem. Soc. 130 4466
Singha N, Das B K, Pramanik B, Das S and Das D 2019 Chem. Sci.10 10035
Black S P, Sanders J K M and Stefankiewicz A R 2014 Chem. Soc. Rev. 43 1861
Wei Y, Thyparambil A A and Latour R A 2014 Biochim. Biophys. Acta: Proteins and Proteomics 1844 2331
Niu L, Liu L, Xi W, Han Q, Li Q, Yu Y et al 2016 ACS Nano 10 4143
Smith A M, Williams R J, Tang C, Coppo P, Collins R F, Turner M L et al 2008 Adv. Mater. 20 37
Madhu C, Roy B, Makam P and Govindaraju T 2018 Chem. Commun. 54 2280
Ohrdorf K-H and Flachberger H 2015 in Polymer nanoclay composites S Laske (ed) (Oxford: William Andrew Publishing) p 1
Tanner R I and Walters K 1998 in Rheology series (Amsterdam: Elsevier) Vol 7, p 159
Acknowledgements
DD acknowledges financial support from SERB, India (EMR/2016/000857).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Dowari, P., Pramanik, B. & Das, D. pH and secondary structure instructed aggregation to a thixotropic hydrogel by a peptide amphiphile. Bull Mater Sci 43, 70 (2020). https://doi.org/10.1007/s12034-019-2027-6
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s12034-019-2027-6