Abstract
Green/sustainable cellulose paper-based platforms at point-of-care testing have received much interest in biomedical fields at resource-limited settings. In such platforms, biomolecules would have to be immobilized onto paper substrate to achieve detection. Therefore, effective molecule immobilization is critical for the performance of paper-based testing, especially the detection sensitivity. Although various methods have been developed to improve the detection sensitivity, the immobilization capacity of biomolecules has not been considered yet. In this study, we developed a facile method for modifying paper materials by incorporating chitosan into NC membrane, which has positive effect on the pore size, porosity, and surface groups of NC membrane, hence, enhancing the immobilization capacity of biomolecules on paper substrate. We demonstrated the effectiveness of the above mentioned approach by using nucleic acid lateral flow assay (NALFAs) as a model paper-based platform; in comparison with the unmodified NALFAs, the results showed ten-fold increase in the detection sensitivity for HBV. We envision that this facile modification method will have a great potential for developing other highly sensitive paper-based detecting platforms.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (21808132), the China Postdoctoral Science Foundation (2018M633525), the Project of Shaanxi Provincial Education department (18JK0096), the Natural Science Basic Research Plan of Shaanxi Province (2019JQ-517), the Opening Project of National Experimental Teaching Demonstration Center of Light Chemical Engineering (2018QGSJ02-10), the High-level Foreign Experts Project (GD20186100425), “Biomass Chemistry and Materials” Academician Workstation Project in SUST (134090002).
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Tang, R.H., Li, M., Liu, L.N. et al. Chitosan-modified nitrocellulose membrane for paper-based point-of-care testing. Cellulose 27, 3835–3846 (2020). https://doi.org/10.1007/s10570-020-03031-x
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DOI: https://doi.org/10.1007/s10570-020-03031-x