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Positive quantitative PCR detecting Fusarium solani in a case of mixed invasive fungal disease due to Mucorales and Fusarium solani

Bone Marrow Transplantation volume 55, pages 873–876 (2020)Cite this article

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Abstract

We present a case of invasive fungal co-infection in a young patient treated for an acute myeloid leukemia and having undergone a twice-haploid matched unrelated donor hematopoietic stem cell transplantation (HSCT) with two different donors. A mucormycosis diagnosis was made shortly after the patient’s admission using imagery and specific Mucorales qPCR which was treated with liposomal amphotericin B and posaconazole. Twenty days later, a blood culture was positive for Fusarium solani, and disseminated cutaneous lesions appeared. The antifungal treatment was changed to liposomal amphotericin B and voriconazole. Thanks to a complete hematological reconstitution and despite a co-infection with two aggressive filamentous opportunistic fungi, the patient recovered. We took advantage of this clinical case to test a specific Fusarium solani qPCR, which proved to be promising when performed retrospectively on some of the patient samples.

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Acknowledgements

We thank Pamela Albert for her editorial assistance. We thank Dr. Olivier Lortholary for his advice on antifungal therapeutic management regarding the MIC result obtained from the National Reference Center (Paris, France).

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Authors and Affiliations

  1. Chrono-Environnement CNRS 6249 Research Team, Franche-Comté University, Besançon, France

    Anne-Pauline Bellanger, Steffi Rocchi, Emeline Scherer & Laurence Millon

  2. Parasitology-Mycology Department, Besançon University Hospital, Besançon, France

    Anne-Pauline Bellanger, Steffi Rocchi, Emeline Scherer & Laurence Millon

  3. Hematology Department, Besançon University Hospital, Besançon, France

    Ana Berceanu

  4. Hematology Department, Dijon University Hospital, Dijon, France

    Fabrice Larosa

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  1. Anne-Pauline Bellanger
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  2. Steffi Rocchi
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  4. Emeline Scherer
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  5. Fabrice Larosa
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Correspondence to Anne-Pauline Bellanger.

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Bellanger, AP., Rocchi, S., Berceanu, A. et al. Positive quantitative PCR detecting Fusarium solani in a case of mixed invasive fungal disease due to Mucorales and Fusarium solani. Bone Marrow Transplant 55, 873–876 (2020). https://doi.org/10.1038/s41409-020-0819-3

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