Abstract
The production of cellulosic ethanol has been gaining attention in the industry sector because of the high availability of lignocellulosic biomass from agricultural and forestry activities. Pinus patula is one of the most typical softwood species in Colombia. The aim of this work is to evaluate the production of ethanol using Pinus patula as raw material using dilute acid pretreatment and enzymatic hydrolysis to produce sugars able to be used as substrate for the strain Saccharomyces cerevisiae. Three fermentation configurations were selected to evaluate the performance of the microorganism: configurations 1 and 2 used glucose in a percentage of 80%w/v and 70%w/v, respectively, as substrate to establish the adaptation requirements of the microorganism. The configuration 3 considered the use of concentrated P. patula hydrolysate. An experimental yield of 0.364 ± 0.009 g ethanol/g sugar (73% of the theoretical) was obtained. Additionally, the economic and energetic comparison between the biochemical (ethanol production through fermentation) and thermochemical (synthesis gas through gasification) pathways to produce bioenergy was performed through simulation approaches. As main results, a higher ethanol production cost (1.53 USD/L) was obtained in comparison to the market price (0.77 USD/L) and a low energy efficiency (20%). Different alternatives such as waste integration and energy incentives must be considered in order to produce ethanol in a feasible way.
Similar content being viewed by others
References
Ford, C.M., Jones, N.B., Chirwa, P.W.: Pinus patula and pine hybrid hedge productivity in South Africa: a comparison between two vegetative propagation systems exposed to natural infection by Fusarium circinatum. J. For. Sci. 2620, 1–9 (2014). https://doi.org/10.2989/20702620.2014.916501
Moncada, J., Cardona, C.A., Higuita, J.C., Vélez, J.J., López-Suarez, F.E.: Wood residue (Pinus patula bark) as an alternative feedstock for producing ethanol and furfural in Colombia: experimental, techno-economic and environmental assessments. Chem. Eng. Sci. 140, 309–318 (2016). https://doi.org/10.1016/j.ces.2015.10.027
García, C.A., Betancourt, R., Cardona, C.A.: Stand-alone and biorefinery pathways to produce hydrogen through gasification and dark fermentation using Pinus patula. J. Environ. Manage. (2015). https://doi.org/10.1016/j.jenvman.2016.04.001
Damartzis, T., Zabaniotou, A.: Thermochemical conversion of biomass to second generation biofuels through integrated process design—a review. Renew. Sustain. Energy Rev. 15, 366–378 (2011). https://doi.org/10.1016/J.RSER.2010.08.003
Johnson, E.: Integrated enzyme production lowers the cost of cellulosic ethanol. Biofuels Bioprod. Biorefin. 10, 164–174 (2016). https://doi.org/10.1002/bbb
Soudham, V.P., Raut, D.G., Anugwom, I., Brandberg, T., Larsson, C., Mikkola, J.P.: Coupled enzymatic hydrolysis and ethanol fermentation: ionic liquid pretreatment for enhanced yields. Biotechnol. Biofuels. 8, 135 (2015). https://doi.org/10.1186/s13068-015-0310-3
Daza Serna, L.V., Orrego Alzate, C.E., Cardona Alzate, C.A.: Supercritical fluids as a green technology for the pretreatment of lignocellulosic biomass. Bioresour. Technol. 199, 113–120 (2016)
Galbe, M., Zacchi, G.: A review of the production of ethanol from softwood. Appl. Microbiol. Biotechnol. 59, 618–628 (2002). https://doi.org/10.1007/s00253-002-1058-9
Hoyer, K., Galbe, M., Zacchi, G.: Production of fuel ethanol from softwood by simultaneous saccharification and fermentation at high dry matter content. J. Chem. Technol. Biotechnol. 84, 570–577 (2009). https://doi.org/10.1002/jctb.2082
St1 Biofuels Oy: Cellunolix® ethanol plant to be built in Finland. https://www.st1.eu/cellunolix-ethanolplant-to-be-built-in-finland
Söderström, J., Pilcher, L., Galbe, M., Zacchi, G.: Two-step pretreatment of softwood with SO2 two-step steam pretreatment of softwood with SO2 impregnation for ethanol production. Appl. Biochem. Biotechnol. 98–100, 5–21 (2002)
St1 Biofuels Oy: St1´s and SOK´s joint venture NEB plans 50-million-litre Cellunolix® bioethanol plant in Pietarsaari. https://www.st1.eu/st1s-and-soks-joint-venture-neb-plans-50-million-litre-cellunolix-bioethanolpla
Aho, A., Kumar, N., Eränen, K., Holmbom, B., Hupa, M., Salmi, T., Murzin, D.Y.: Pyrolysis of softwood carbohydrates in a fluidized bed reactor. Int. J. Mol. Sci. 9(9), 1665–1675 (2008)
Garcìa-Pérez, M., Chaala, A., Pakdel, H., Kretschmer, D., Roy, C.: Vacuum pyrolysis of softwood and hardwood biomass: comparison between product yields and bio-oil properties. J. Anal. Appl. Pyrolysis. 78, 104–116 (2007). https://doi.org/10.1016/J.JAAP.2006.05.003
Oasmaa, A., Solantausta, Y., Arpiainen, V., Kuoppala, E., Sipilä, K.: Fast pyrolysis bio-oils from wood and agricultural residues. Energy Fuels. 24, 1380–1388 (2010). https://doi.org/10.1021/ef901107f
Amaral, S., De Carvalho Junior, A., Costa, M.A.M., Neto, T.G.S., Dellani, R., Leite, L.H.S.: Comparative study for hardwood and softwood forest biomass: chemical characterization, combustion phases and gas and particulate matter emissions. Bioresour. Technol. 164, 55–63 (2014). https://doi.org/10.1016/j.biortech.2014.04.060
Roy, M.M., Corscadden, K.W.: An experimental study of combustion and emissions of biomass briquettes in a domestic wood stove. Appl. Energy. 99, 206–212 (2012). https://doi.org/10.1016/J.APENERGY.2012.05.003
García, C.A., Morales, M., Quintero, J., Aroca, G., Cardona, C.A.: Environmental assessment of hydrogen production based on Pinus patula plantations in Colombia. Energy. 139, 606–616 (2017). https://doi.org/10.1016/j.energy.2017.08.012
Waldner, M.H., Vogel, F.: Renewable production of methane from woody biomass by catalytic hydrothermal gasification. Ind. Eng. Chem. Res. 44, 4543–4551 (2005). https://doi.org/10.1021/ie050161h
Mandl, C., Obernberger, I., Scharler, I.R.: Characterisation of fuel bound nitrogen in the gasification process and the staged combustion of producer gas from the updraft gasification of softwood pellets. Biomass Bioenergy 35, 4595–4604 (2011). https://doi.org/10.1016/J.BIOMBIOE.2011.09.001
Moncada, J., Tamayo, J., Cardona, C.A.: Evolution from biofuels to integrated biorefineries: techno-economic and environmental assessment of oil palm in Colombia. J. Clean. Prod. 81, 51–59 (2014)
Chum, H., Faaij, A., Moreira, J.: Bioenergy. In: Edenhofer, O., Pichs-Madruga, R., Sokona, Y. (eds.) Renewable Energy Sources and Climate Change Mitigation. pp. 46–55. Intergovernmental Panel on Climate Change (IPCC), Geneva (2011)
García-Velásquez, C.A.: Hydrogen production through gasification and dark fermentation. (2016). http://www.bdigital.unal.edu.co/54321/
Timung, R., Mohan, M., Chilukoti, B., Sasmal, S., Banerjee, T., Goud, V.V.: Optimization of dilute acid and hot water pretreatment of different lignocellulosic biomass: a comparative study. Biomass Bioenergy 81, 9–18 (2015). https://doi.org/10.1016/j.biombioe.2015.05.006
Karimi, K., Taherzadeh, M.J.: A critical review of analytical methods in pretreatment of lignocelluloses: composition, imaging, and crystallinity. Bioresour. Technol. 200, 1008–1018 (2016). https://doi.org/10.1016/j.biortech.2015.11.022
Selig, M., Weiss, N., Ji, Y.: Enzymatic Saccharification of Lignocellulosic Biomass, pp. 1–5. National Renewable Energy Laboratory, Golden (2008)
Miller, G.L.: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426–428 (1959). https://doi.org/10.1021/ac60147a030
Jensen, J., Morinelly, J., Aglan, A., Mix, A., Shonnard, D.: Kinetic characterization of biomass dilute sulfuric acid hydrolysis: mixtures of hardwoods, softwood, and switchgrass. Environ. Energy Eng. 54, 1637–1645 (2008). https://doi.org/10.1002/aic
Esteghlalian, A., Hashimoto, A.G., Fenske, J.J., Penner, M.H.: Modeling and optimization of the dilute-sulfuric-acid pretreatment of corn stover, poplar and switchgrass. Bioresour. Technol. 59, 129–136 (1997). https://doi.org/10.1016/S0960-8524(97)81606-9
Wooley, R.J., Putsche, V.: Development of an ASPEN PLUS physical property database for biofuels components, pp.1–38. National Renewable Energy Laboratory, Golden (1996)
García, C.A., Peña, Á, Betancourt, R., Cardona, C.A.: Energetic and environmental assessment of thermochemical and biochemical ways for producing energy from agricultural solid residues: coffee cut-stems case. J. Environ. Manage. (2017). https://doi.org/10.1016/j.jenvman.2017.04.029
Quintero, J.a., Moncada, J., Cardona, C.a.: Techno-economic analysis of bioethanol production from lignocellulosic residues in Colombia: a process simulation approach. Bioresour. Technol. 139, 300–307 (2013). https://doi.org/10.1016/j.biortech.2013.04.048
Moncada, J., Tamayo, J.A., Cardona, C.A.: Integrating first, second, and third generation biorefineries: incorporating microalgae into the sugarcane biorefinery. Chem. Eng. Sci. 118, 126–140 (2014). https://doi.org/10.1016/j.ces.2014.07.035
Rafiqul, I.S.M., Mimi Sakinah, aM.: Kinetic studies on acid hydrolysis of Meranti wood sawdust for xylose production. Chem. Eng. Sci. 71, 431–437 (2012). https://doi.org/10.1016/j.ces.2011.11.007
Khodaverdi, M., Karimi, K., Jeihanipour, A., Taherzadeh, M.J.: Kinetic modeling of rapid enzymatic hydrolysis of crystalline cellulose after pretreatment by NMMO. J. Ind. Microbiol. Biotechnol. 39, 429–438 (2012). https://doi.org/10.1007/s10295-011-1048-y
Kadam, K.L., Rydholm, E.C., McMillan, J.D.: Development and validation of a kinetic model for enzymatic saccharification of lignocellulosic biomass. Biotechnol. Prog. 20, 698–705 (2004). https://doi.org/10.1021/bp034316x
Quintero, J.A., Cardona, C.A.: Process Simulation of Fuel Ethanol Production from Lignocellulosics using Aspen Plus. Ind. Eng. Chem. Res. 50(10), 6205–6212 (2011)
Pitt, W.W., Haag, G.L., Lee, D.D.: Recovery of ethanol from fermentation broths using selective sorption-desorption. Biotechnol. Bioeng. 25, 123–131 (1983). https://doi.org/10.1002/bit.260250110
García, C.A., Moncada, J., Aristizábal Marulanda, V., Cardona, C.A.: Techno-economic and energetic assessment of hydrogen production through gasification in the Colombian context: coffee cut-stems. Int. J. Hydrogen Energy. 42, 5849–5864 (2017). https://doi.org/10.1016/j.ijheatmasstransfer.2017.09.038
Melgar, A., Pérez, J.F., Laget, H., Horillo, A.: Thermochemical equilibrium modelling of a gasifying process. Energy Convers. Manag. 48, 59–67 (2007). https://doi.org/10.1016/j.enconman.2006.05.004
Jarungthammachote, S., Dutta, A.: Equilibrium modeling of gasification: Gibbs free energy minimization approach and its application to spouted bed and spout-fluid bed gasifiers. Energy Convers. Manag. 49, 1345–1356 (2008). https://doi.org/10.1016/j.enconman.2008.01.006
Peters, M.S., Timmerhaus, K.D., West, R.E.: Plant Design and Economics for Chemical Engineers. McGraw-Hill, New York (2004)
Fuels, F.: Wood chip and wood pellet price comparison. https://www.forestfuels.co.uk/wood-fuel-pricecomparison/
Kemcore: Reagents Market Price. https://www.kemcore.com/sulphuric-acid-98.html
Liu, G., Zhang, J., Bao, J.: Cost evaluation of cellulase enzyme for industrial-scale cellulosic ethanol production based on rigorous Aspen Plus modeling. Bioprocess. Biosyst. Eng. 39, 133–140 (2016). https://doi.org/10.1007/s00449-015-1497-1
Federación Nacional de Biocombustibles de Colombia—Fedebiocombustibles: Biofuel prices in Colombia 2016–2017
Ulrich, G.D., Vasudevan, P.T.: How to estimate utility costs. Chem. Eng. 113, 66–69 (2006)
Boundy, B.: Biomass Energy Data Book, 4 ed, p. 254. Deparment of Energy, Washington DC, (2011)
Kim, K.H.: Two-stage dilute acid-catalyzed hydrolytic conversion of softwood sawdust into sugars fermentable by ethanologenic microorganisms. J. Sci. Food Agric. 85, 2461–2467 (2005). https://doi.org/10.1002/jsfa.2268
Bösch, P., Wallberg, O., Joelsson, E., Galbe, M., Zacchi, G.: Research impact of dual temperature profile in dilute acid hydrolysis of spruce for ethanol production. Biotechnol. Biofuels. 3, 15 (2010). https://doi.org/10.1186/1754-6834-3-15
Shinozaki, Y., Kitamoto, H.K.: Ethanol production from ensiled rice straw and whole-crop silage by the simultaneous enzymatic saccharification and fermentation process. J. Biosci. Bioeng. 111, 320–325 (2011). https://doi.org/10.1016/j.jbiosc.2010.11.003
Dinh, T.N., Nagahisa, K., Hirasawa, T., Furusawa, C., Shimizu, H.: Adaptation of Saccharomyces cerevisiae cells to high ethanol concentration and changes in fatty acid composition of membrane and cell size. PLoS ONE. 3, e2623 (2008). https://doi.org/10.1371/journal.pone.0002623
Alvira, P., Tomás-Pejó, E., Ballesteros, M., Negro, M.J.: Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour. Technol. 101, 4851–4861 (2010). https://doi.org/10.1016/j.biortech.2009.11.093
Qian, M., Tian, S., Li, X., Zhang, J., Pan, Y., Yang, X.: Ethanol production from dilute-acid softwood hydrolysate by co-culture. Appl. Biochem. Biotechnol. (2006). https://doi.org/10.1385/ABAB:134:3:273
Nyangi Chacha: Comparison of Escherichia coli KO11 and Saccharomyces cerevisiae ATCC 96581 in fermenting Pinus patula hydrolysate pretreated at different steam explosion severity. Afr. J. Biotechnol. (2012). https://doi.org/10.5897/AJB11.3498
Hawkins, G.M., Doran-Peterson, J.: A strain of Saccharomyces cerevisiae evolved for fermentation of lignocellulosic biomass displays improved growth and fermentative ability in high solids concentrations and in the presence of inhibitory compounds. Biotechnol. Biofuels. 4, 49 (2011). https://doi.org/10.1186/1754-6834-4-49
Nguyen, Q.A., Tucker, M.P., Keller, F.A., Beaty, D.A., Connors, K.M., Eddy, F.P.: Dilute acid hydrolysis of softwoods. Appl. Biochem. Biotechnol. (1999). https://doi.org/10.1385/ABAB:77:1-3:133
Mesa, L., Martínez, Y., Barrio, E., González, E.: Desirability function for optimization of dilute acid pretreatment of sugarcane straw for ethanol production and preliminary economic analysis based in three fermentation configurations. Appl. Energy. 198, 299–311 (2017). https://doi.org/10.1016/j.apenergy.2017.03.018
Parajó, J.C., Vázquez, D., Alonso, J.L., Santos, V., Dominguez, H.: Prehydrolysis of Eucalyptus wood with dilute sulphuric acid: operation at atmospheric pressure. Holz als Roh- und Werkst. 51, 357–363 (1993). https://doi.org/10.1007/BF02663809
Daystar, J., Treasure, T., Gonzalez, R., Reeb, C., Venditti, R., Kelley, S.: The NREL biochemical and thermochemical ethanol conversion processes: financial and environmental analysis comparison. BioResources. 10, 5096–5116 (2015). https://doi.org/10.15376/biores.10.3.5096-5116
Zhao, L., Zhang, X., Xu, J., Ou, X., Chang, S., Wu, M.: Techno-economic analysis of bioethanol production from lignocellulosic biomass in china: dilute-acid pretreatment and enzymatic hydrolysis of corn stover. Energies. 8, 4096–4117 (2015). https://doi.org/10.3390/en8054096
Foust, T.D., Aden, A., Dutta, A., Phillips, S.: An economic and environmental comparison of a biochemical and a thermochemical lignocellulosic ethanol conversion processes. Cellulose. 16, 547–565 (2009). https://doi.org/10.1007/s10570-009-9317-x
Piccolo, C., Bezzo, F.: A techno-economic comparison between two technologies for bioethanol production from lignocellulose. Biomass Bioenergy 33, 478–491 (2009). https://doi.org/10.1016/j.biombioe.2008.08.008
Wingren, A., Söderström, J., Galbe, M., Zacchi, G.: Process considerations and economic evaluation of two-step steam pretreatment for production of fuel ethanol from softwood. Biotechnol. Prog. 20, 1421–1429 (2004). https://doi.org/10.1021/bp049931v
Unidad de Planeación Energética (UPME): Informe Mensual De Variables De Generación Y Del Mercado Eléctrico Colombiano., Bogotá, Colombia (2015)
Acknowledgements
The authors express their acknowledgments to the Centro de Bioinformática y Biología Computacional (BIOS) for the financial support through the project entitled “Fortalecimiento de CTEI en biotecnologia para el departamento de Caldas apoyado por infraestructura computacional avanzada y trabajo colaborativo (CALDAS BIOREGION)” Grant No. 08112013-0621. The authors also express their gratitude to the Universidad Nacional de Colombia Sede Manizales through the Projects entitled “Development of modular small-scale integrated biorefineries to produce an optimal range of bioproducts from a variety of rural agricultural and agroindustrial residues/wastes with a minimum consumption of fossil energy—SMIBIO” from ERANET LAC 2015 Grant No. 202010011331 and the Project “Techno-economic and environmental evaluation of a biorefinery using the residues from the Coffee Crop” Grant No. 202010014230.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
García-Velásquez, C.A., Carmona-Garcia, E., Caballero, A.S. et al. Fermentative Production of Ethanol Using Pinus patula as Raw Material: Economic and Energy Assessment. Waste Biomass Valor 11, 1777–1788 (2020). https://doi.org/10.1007/s12649-018-0494-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12649-018-0494-4