Abstract
Male Aegus chelifer chelifer stag beetles are normally equipped with long mandibles as a weapon to compete with rival males over females, where fighting or aggressive behavior is generally expected during the encounter between two male stag beetles. However, three main patterns were observed between 164 pairings of male stag beetles. Besides the predominant aggressive or fighting behavior (107 pairings), male stag beetles also exhibited disregarding behavior (29 pairings) and same-sex sexual behavior (28 pairings). For sexual interactions, active males exhibited courting and mating behavior as found in male-female interactions, but the insemination process was unsuccessful. Responses of passive males varied from totally passive to resisting and running away. Visual confusion of short-mandible males for females by active males was not likely to be responsible for the same-sex sexual behavior because the correlation between body size and the sexual behavior or sexual role between male-male pairs was not significant. Rather, sexual recognition via chemicals on the cuticle surface might be responsible for the same-sex sexual behavior.
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References
Bailey NW, French N (2012) Same-sex sexual behaviour and mistaken identity in male field crickets, Teleogryllus oceanicus. Anim Behav 84:1031–1038
Bailey NW, Zuk M (2009) Same-sex sexual behavior and evolution. Trends Ecol Evol 24:439–446
Briffa M (2008) Decisions during fights in the house cricket, Acheta domesticus: mutual or self assessment of energy, weapons and size? Anim Behav 75:1053–1062
Eberhard WG, Gutierrez EE (1991) Male dimorphisms in beetles and earwigs and the question of developmental constraints. Evolution 45:18–28
Egge AR, Brandt Y, Swallow JG (2011) Sequential analysis of aggressive interactions in the stalk-eyed fly Teleopsis dalmanni. Behav Ecol Sociobiol 65:369–379
Ek-Amnuay P (2008) Beetles of Thailand. 2 edn. Amarin Printing and Publishing Public, Bangkok
Ek-Amnuay P (2009) Breeding and Rearing Beetles. Amarin Printing and Publishing Public, Bangkok
Emlen D (2008) The evolution of animal weapons. Annual Review of Ecology, Evolution, Systematics and Biodiversity 39:387–413
Emlen DJ (1997) Alternative reproductive tactics and male-dimorphism in the horned beetle Onthophagus acuminatus (Coleoptera: Scarabaeidae). Behav Ecol Sociobiol 41:335–341
Field KL, Waite TA (2004) Absence of female conspecifics induces homosexual behaviour in male guppies. Anim Behav 68:1381–1389
Gotoh H, Cornette R, Koshikawa S, Okada Y, Lavine LC, Emlen DJ, Miura T (2011) Juvenile hormone regulates extreme mandible growth in male stag beetles. PLoS One 6:e21139
Gotoh H, Fukaya K, Miura T (2012) Heritability of male mandible length in the stag beetle Cyclommatus metallifer. Entomol Sci 15:430–433
Goyens J, Dirckx J, Aerts P (2015a) Costly sexual dimorphism in Cyclommatus metallifer stag beetles. Funct Ecol 29:35–43
Goyens J, Dirckx J, Aerts P (2015b) Stag beetle battle behavior and its associated anatomical adaptations. J Insect Behav 28:227–244
Harvey DJ, Hawes CJ, Gange AC, Finch P, Chesmore D, Farr I (2011) Development of non-invasive monitoring methods for larvae and adults of the stag beetle, Lucanus cervus. Insect Conserv Diver 4:4–14
Hoem SA, Melis C, Linnell JD, Andersen R (2007) Fighting behaviour in territorial male roe deer Capreolus capreolus: the effects of antler size and residence. Eur J Wildl Res 53:1–8
Hongo Y, Okamoto K (2013) Interspecific contests between males of two Japanese stag beetle species, Lucanus maculifemoratus and Prosopocoilus inclinatus: what overcomes a body size disadvantage? Behaviour 150:39–59
Iguchi Y (1996) Sexual behavior of the horned beetle, Allomyrina dichotoma septentrionalis (Coleoptera, Scarabaeidae). Jpn J Entomol 64:870–875
Inoue A, Hasegawa E (2013) Effect of morph types, body size and prior residence on food-site holding by males of the male-dimorphic stag beetle Prosopocoilus inclinatus (Coleoptera: Lucanidae). J Ethol 31:55–60
Karino K, Niiyama H, Chiba M (2005) Horn length is the determining factor in the outcomes of escalated fights among male Japanese horned beetles, Allomyrina dichotoma L.(Coleoptera: Scarabaeidae). J Insect Behav 18:805–815
Kijimoto T, Pespeni M, Beckers O, Moczek AP (2013) Beetle horns and horned beetles: emerging models in developmental evolution and ecology. WIREs Dev Biol 2:405–418
Kim SI, Farrell BD (2015) Phylogeny of world stag beetles (Coleoptera: Lucanidae) reveals a Gondwanan origin of Darwin’s stag beetle. Mol Phylogenet Evol 86:35–48
Lahav S, Soroker V, Hefetz A, Vander Meer RK (1999) Direct behavioral evidence for hydrocarbons as ant recognition discriminators. Naturwissenschaften 86:246–249
Levan KE, Fedina TY, Lewis SM (2009) Testing multiple hypotheses for the maintenance of male homosexual copulatory behaviour in flour beetles. J Evol Biol 22:60–70
MacFarlane GR, Blomberg SP, Vasey PL (2010) Homosexual behaviour in birds: frequency of expression is related to parental care disparity between the sexes. Anim Behav 80:375–390
McCullough EL, Tobalske BW, Emlen DJ (2014) Structural adaptations to diverse fighting styles in sexually selected weapons. Proc Natl Acad Sci U S A 111:14484–14488
McRobert SP, Tompkins L (1988) Two consequences of homosexual courtship performed by Drosophila melanogaster and Drosophila affinis males. Evolution 42:1093–1097
Mizunuma T, Nagai S (1994) Sekai no Kuwagatamushi Daizukan: the Lucanid beetles of the world. Mushisha, Tokyo
Moczek AP (2002) Allometric plasticity in a polyphenic beetle. Ecol Entomol 27:58–67
Mori H, Chiba S (2009) Sociality improves larval growth in the stag beetle Figulus binodulus (Coleoptera: Lucanidae). Eur J Entomol 106:379–383
Nagamoto J, Aonuma H, Hisada M (2005) Discrimination of conspecific individuals via cuticular pheromones by males of the cricket Gryllus bimaculatus. Zool Sci 22:1079–1088
Obata S, Hidaka T (1983) Recognition of opponent and mate in Japanese horned beetle, Allomyrina dichotoma L.(Coleoptera, Scarabaeidae). Kontyû, Tokyo 51:534–538
Ortiz-Domínguez M, Favila ME, Mendoza-López MR, García-Barradas O, Cruz-Sánchez JS (2006) Epicuticular compounds and sexual recognition in the ball-roller scarab, Canthon cyanellus cyanellus. Entomol Exp Appl 119:23–27
Pinratana A, Maes J-M (2003) Lucanidae of Thailand. Sunprinting, Bangkok
Preston-Mafham K (2006) Post-mounting courtship and the neutralizing of male competitors through “homosexual” mountings in the fly Hydromyza livens F.(Diptera: Scatophagidae). J Nat Hist 40:101–105
R Development Core Team (2016) R: A Language and Environment for Statistical Computing
Rink M, Sinsch U (2007) Radio-telemetric monitoring of dispersing stag beetles: implications for conservation. J Zool 272:235–243
Rohlf FJ (2013) tpsDIG version 2.17. Department of Ecology and Evolution, State University of New York at stony brook, New York
Scharf I, Martin OY (2013) Same-sex sexual behavior in insects and arachnids: prevalence, causes, and consequences. Behav Ecol Sociobiol 67:1719–1730
Shiokawa T, Iwahashi O (2000) Mating success of small sized males of Japanese stag beetle Prosopocoilus dissimilis okinawanus Nomura. Jap J Entomol (New Ser) 3:157–165
Singer TL (1998) Roles of hydrocarbons in the recognition systems of insects. Am Zool 38:394–405
Siva-Jothy MT (1987) Mate securing tactics and the cost of fighting in the Japanese horned beetle, Allomyrina dichotoma L.(Scarabaeidae). J Ethol 5:165–172
Songvorawit N, Butcher BA, Chaisuekul C (2017) Different allometric intercepts in major Aegus chelifer chelifer stag beetle males from urban and forest habitats. J Asia Pac Entomol 20:835–839
Songvorawit N, Butcher BA, Chaisuekul C (2018) Resource holding potential and the outcome of aggressive interactions between paired male Aegus chelifer chelifer (Coleoptera: Lucanidae) stag beetles. J Insect Behav 31:347–360
Switzer PV, Forsythe PS, Escajeda K, Kruse KC (2004) Effects of environmental and social conditions on homosexual pairing in the Japanese beetle (Popillia japonica Newman). J Insect Behav 17:1–16
Van Gossum H, De Bruyn L, Stoks R (2005) Reversible switches between male–male and male–female mating behaviour by male damselflies. Biol Lett 1:268–270
Vasey PL (1995) Homosexual behavior in primates: a review of evidence and theory. Int J Primatol 16:173–204
Vasey PL, Chapais B, Gauthier C (1998) Mounting interactions between female Japanese macaques: testing the influence of dominance and aggression. Ethology 104:387–398
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This research was supported by the Grants for Development of New Faculty Staff, Ratchadaphiseksomphot Endowment Fund, Chulalongkorn University.
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Songvorawit, N., Butcher, B.A. & Chaisuekul, C. Size Does Not Matter: Same-Sex Sexual Behavior Occurred Regardless of Mandible Size in Male Stag Beetle Aegus chelifer chelifer (Coleoptera: Lucanidae). J Insect Behav 32, 282–289 (2019). https://doi.org/10.1007/s10905-019-09733-w
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DOI: https://doi.org/10.1007/s10905-019-09733-w