Abstract
The persistence and movement of transgenic DNA in agricultural and natural systems is largely unknown. This movement poses a threat of horizontal gene transfer and possible proliferation of genetically modified DNA into the general environment. To assess the persistence of transgenic DNA in a field of Roundup Ready® corn, we quantified the presence of the transgene for glyphosate tolerance within a soil food web. Using quantitative real-time PCR, we identified the cp4 epsps transgene in bulk soil microarthropods, nematodes, macroarthropods and earthworms sampled within the corn cropping system. We found evidence of the transgene at all dates and in all animal groups. Transgenic DNA concentration in animal was significantly higher than that of background soil, suggesting the animals were feeding directly on transgenic plant material. It remains to be tested whether this DNA was still within the plant residues, present as free, extracellular DNA or had already undergone genetic transformation into competent bacterial cells. These results are the first to demonstrate the persistence of transgenic crop DNA residues within a food web.
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References
Andow D.A,. Zwahlen C. (2006) Assessing environmental risks of transgenic plants, Ecol. Lett. 9, 196–214.
Bohan D.A., Boffey C.W.H., Brooks D.R., Clark S.J., Dewar A.M., Firbank L.G., Haughton A.J., Hawes C., Heard M.S., May M.J., Osborne J.L., Perry J.N., Rothery P., Roy D.P., Scott R.J., Squire G.R., Woiwod, I.P. and Champion G.T. (2005) Effects on weed and invertebrate abundance and diversity of herbicide management in genetically modified herbicide-tolerant winter-sown oilseed rape, Proc. R. Soc. B 272, 463–474.
Brooks D.R., Bohan D.A., Champion G.T., Haughton A.J., Hawes C., Heard M.S., Clark S.J., Dewar A.M., Firbank L.G., Perry J.N., Rothery P., Scott R.J., Woiwod I.P., Birchall C., Skellern M.P., Walker J.H., Baker, P., Bell D., Browne E.L., Dewar A.J.G., Fairfax C.M., Garner, B.H., Haylock L.A., Horne S.L., Hulmes S.E., Mason N.S., Norton L.R., Nuttall P., Randle Z., Rossall M.J., Sands R.J.N., Singer E.J., Walker M.J. (2005) Invertebrate responses to the management of genetically modified herbicide-tolerant and conventional spring crops. I. Soil-surface-active invertebrates, Philos. T. Roy. Soc. B 358, 1847–1862.
de Vries J., Wackernagel W. (2004) Microbial horizontal gene transfer and the DNA release from transgenic crop plants, Plant Soil 226, 91–104.
Donegan K.K., Seidler R.J., Fieland V.J., Schaller D.L., Palm C.J., Ganio L.M., Cardwell D.M., Edwards C.A. (1991) The assessment of populations of soil inhabiting invertebrates, Agr. Ecosyst. Environ. 34, 145–176.
Edwards C.A. (1991) The assessment of populations of soil inhabiting invertebrates, Agr. Ecosyst. Environ., 145–176.
Gebhard F., Smalla K. (1999) Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer, FEMS Microbiol. Ecol. 28, 261–272.
Gulden R.H., Lerat, S., Blackshaw, R.E., Powell J.R., Levy Booth D., Dunfield K.E., Trevors J.T., Pauls K.P., Klironomos J.N., Swanton C.J. (2008) Factors affecting the presence and persistence of plant DNA in the soil environment in corn and soybean rotations, Weed Sci. 56, 767–774.
Gulden R.H., Lerat S., Hart M.M., Powell J.R., Trevors J.T., Pauls K.P., Klironomos J.N., Swanton C.J. (2005) Quantitation of transgenic plant DNA in leachate water: Real-time polymerase chain reaction analysis, J. Agr. Food Chem. 53, 5858–5865.
Heinnemann J.A., Traavik T. (2004) Problems in monitoring horizontal gene transfer in field trials of transgenic plants, Nat. Biotechnol. 22, 1105–1110.
Kowalchuk G.A., Bruinsma M., van Veen J.A. (2003) Assessing responses of soil microorganisms to GM plants, Trends Ecol. Evol. 18, 403–410.
Lerat S., England L.S., Vincent M.L., Pauls K.P., Swanton C.J., Klironomos J.N., Trevors J.T. (2005) Real-time polymerase chain reaction (PCR) quantification of the transgenes for Roundup Ready corn and Roundup Ready soybean in soil samples, J. Agr. Food Chem. 53, 1337–1342.
Levy-Booth D.J., Campbell R.G., Gulden R.H., Hart M.M., Powell J.R., Klironomos J.N., Pauls K.P., Swanton C.J., Trevors J.T., Dunfield K.E. (2007) The DNA Cycle in Soil: free DNA in the soil environment, Soil Biol. Biochem. 39, 2977–2991.
Liphadzi K.B.K., Al-Khatib C.N., Bensch P.W., Stahlman J.A., Dille T., Todd C.W., Rice M.J., Horak M.J., Head G. (2005) Soil microbial and nematode communities as affected by glyphosate and tillage practices in a glyphosate-resistant cropping system, Weed Sci. 53, 536–545.
Loureiro I., Escorial C., Garcia-Baudin J.M., Chueca C. (2009) Hybridization, fertility and herbicide resistance of hybrids between wheat and Aegilops biuncialis, Agron. Sustain. Environ. 29, 237–245.
Marvier M., Van Acker R.C. (2005) Can crop transgenes be kept on a leash? Front. Ecol. Environ. 3, 93–100.
Nielsen K.M., Bones A.M., Smalla K., van Elsas J.D. (1998) Horizontal gene transfer from transgenic plants to terrestrial bacteria — a rare event? FEMS Microbiol. Rev. 22, 79–103.
Obryki J.J., Losey J.E., Taylor O.R., Jesse C.C. (2001) Transgenic insecticidal corn: beyond insecticidal toxicity to ecological complexity, Bioscience 51, 353–361.
Steinberger Y. (1997) Decomposition of genetically engineered tobacco under field conditions: persistence of the proteinase inhibitor I product and effects of soil microbial respiration and protozoa, nematode and microarthropod populations, J. Appl. Ecol. 34, 767–777.
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Hart, M.M., Powell, J.R., Gulden, R.H. et al. Detection of transgenic cp4 epsps genes in the soil food web. Agron. Sustain. Dev. 29, 497–501 (2009). https://doi.org/10.1051/agro/2009020
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DOI: https://doi.org/10.1051/agro/2009020