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Acetogenin-Based Formulated Bioinsecticides on Anastrepha fraterculus: Toxicity and Potential Use in Insecticidal Toxic Baits

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Abstract

The present study evaluated the lethal toxicity and oviposition deterrence of ethanolic extracts of Annona mucosa Jacq., Annona muricata L., and Annona sylvatica A. St.-Hil on Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) compared with those of a limonoid-based bioinsecticide (Azamax™ 1.2 EC—azadiractin +3-tigloyl-azadiractol) and a synthetic spinosyn-based insecticide (Delegate™ 250 WG—spinetoram). In addition, the efficacy of the selected toxic bait formulations was evaluated by mixing them with food attractants (Anamed™, 3% Biofruit and 7% sugarcane molasses). In the topical application and ingestion bioassays (2000 mg L−1), the aqueous emulsion of the A. mucosa extract caused greater than 80% mortality of A. fraterculus adults in a similar manner to the spinosyn-based synthetic insecticide. Concentration-response curves were performed for the most promising treatments and showed an activity level dependent on the mode of contamination, exposure time, and applied concentration. In bioassays with and without choice, the A. mucosa (77%), A. muricata (51%), A. sylvatica (60%), Azamax™ (74%), and Delegate™ 250 WG (100%) significantly reduced the number of punctures and galleries in grape berries. In combination with the food attractants Anamed™, 3% Biofruit, and 7% sugarcane molasses, the emulsion of the A. mucosa extract had a residual effect similar to that of the spinetoram insecticide, with a mortality rate of over 80% of A. fraterculus adults up to 14 days after application (DAA) in the absence of rain. Thus, acetogenin-rich formulations, especially from A. mucosa seeds, are useful alternatives for the integrated management of A. fraterculus in agricultural orchards.

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Author contribution statement

DB, DEN, and LdPR planed and designed research; PS, DdCO, LNM, and FCSG conducted experiments; DB, MR, LdPR, and DEN conducted statistical analysis and wrote the manuscript. All authors read and approved the manuscript.

Funding

The authors would like to thank the Rio Grande do Sul Research Support Foundation (FAPERGS, project 17 / 2551-0000778-6) for their financial support for research execution and scholarship provision.

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Authors and Affiliations

  1. Department of Plant Protection, Federal Univ of Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brasil

    P. Stupp, M. Rakes, D. C. Oliveira, L. N. Martins, F. C. S. Geisler & D. Bernardi

  2. Research Center for Family Agriculture, Agricultural Research and Rural Extension Company of Santa Catarina (EPAGRI/CEPAF), Florianópolis, Santa Catarina, Brasil

    L. P. Ribeiro

  3. Brazilian Agricultural Research Corporation (EMBRAPA - Clima Temperado, CPACT), Pelotas, Rio Grande do Sul, Brasil

    D. E. Nava

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  1. P. Stupp
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  2. M. Rakes
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  3. D. C. Oliveira
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Correspondence to D. Bernardi.

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Stupp, P., Rakes, M., Oliveira, D.C. et al. Acetogenin-Based Formulated Bioinsecticides on Anastrepha fraterculus: Toxicity and Potential Use in Insecticidal Toxic Baits. Neotrop Entomol 49, 292–301 (2020). https://doi.org/10.1007/s13744-019-00747-9

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