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Uniaxial extensional viscosity of semidilute DNA solutions

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Abstract

The extensional rheology of polymer melts and dilute polymer solutions has been extensively examined through experiments and theoretical predictions. However, a systematic study of the extensional rheology of polymer solutions in the semidilute regime, in terms of examining the effects of concentration and molecular weight, has not been carried out so far. Previous experimental studies of the shear rheology of semidilute polymer solutions have demonstrated that their behaviour is distinctively different from that observed in the dilute and concentrated regimes. This difference in behaviour is anticipated to be even more pronounced in extensional flows, which play a critical role in a number of industrial contexts such as fiber spinning and ink-jet printing. In this work, the extensional rheology of linear, double-stranded DNA molecules, spanning an order of magnitude of molecular weights (25-289 kilobasepairs) and concentrations (0.03-0.3 mg/ml), has been investigated. DNA solutions are now used routinely as model polymeric systems due to their near-perfect monodispersity. Measurements have been carried out with a filament stretching rheometer since it is the most reliable method for obtaining an estimate of the elongational stress growth of a polymer solution. Transient and steady-state uniaxial extensional viscosities of DNA dissolved in a solvent under excess salt conditions, with a high concentration of sucrose in order to achieve a sufficiently high solvent viscosity, have been determined in the semidilute regime at room temperature. The dependence of the steady state uniaxial extensional viscosity on molecular weight, concentration and extension rate is measured with a view to determining if data collapse can be observed with an appropriate choice of variables. Steady state shear viscosity measurements suggest that sucrose-DNA interactions might play a role in determining the observed rheological behaviour of semidilute DNA solutions with sucrose as a component in the solvent.

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Acknowledgments

This work was supported by the ARC Discovery Projects grant arrangement (Project No. DP120101322). Douglas Smith at UCSD and Brad Olsen at MIT are thanked for their generous gifts of the originally synthesized 25 kbp Fosmid and 289 kbp BAC DNA constructs as agar stab cultures of E. coli. Authors would like to acknowledge Michael Danquah (previously at Monash University), for facilitating lab space as well as DNA isolation amenities. IIT-B Monash research Academy at IIT Bombay, India is thanked for funding and overall provisions. We also thank the anonymous referee for helpful suggestions that have improved the quality of the paper.

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Authors and Affiliations

  1. IITB-Monash Research Academy, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India

    Sharadwata Pan, P. Sunthar, T. Sridhar & J. Ravi Prakash

  2. Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India

    Sharadwata Pan & P. Sunthar

  3. Department of Chemical Engineering, Monash University, Melbourne, VIC, 3800, Australia

    Sharadwata Pan, Duc At Nguyen, T. Sridhar & J. Ravi Prakash

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  1. Sharadwata Pan
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  2. Duc At Nguyen
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  3. P. Sunthar
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Correspondence to J. Ravi Prakash.

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This paper is based on an invited lecture presented by the corresponding author at the 30th Anniversary Symposium of the Korean Society of Rheology (The 18th International Symposium on Applied Rheology (ISAR)), held on May 21-24, 2019, Seoul.

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Pan, S., Nguyen, D.A., Sunthar, P. et al. Uniaxial extensional viscosity of semidilute DNA solutions. Korea-Aust. Rheol. J. 31, 255–266 (2019). https://doi.org/10.1007/s13367-019-0026-1

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