Skip to main content
Log in

The responses of carbon- and nitrogen-acquiring enzymes to nitrogen and phosphorus additions in two plantations in southern China

  • Original Paper
  • Published:
Journal of Forestry Research Aims and scope Submit manuscript

Abstract

In tropical plantations, nutrients such as nitrogen (N) or phosphorus (P) are often applied as management practices. However, the effects of such nutrient additions on topsoil C- and N-acquiring enzymes activities are unclear. In this study, the impacts of fertilization on β-1,4-glucosidase (BG), β-D-cellobiosidase (CBH), β-1,4-xylosidase (BX), β-1,4-N-acetyl-glucosaminidase (NAG), and leucine amino peptidase (LAP) enzymes activities from topsoil and litter layer of two tropical plantations (Acacia auriculiformis and Eucalyptus urophylla) were measured. The results showed that N addition had neutral impact on topsoil enzymes, while significantly elevating the activities of BG, CBH, BX, and NAG in the litter layer. P fertilization had no impacts except for an elevation of NAG in litter sample. There was no interactions found between N and P additions on these enzyme activities. The clearer impacts of N over P fertilization were unexpected because that the study site receives a high rate of atmospheric N deposition, and has low soil P availability. The impact of P fertilization on hydrolytic enzyme activities may be less important compared with that of N.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Arai S, Ishizuka S, Ohta S, Saifuddin A, Naoko T, Nagaharu T, Arisman H (2008) Potential N2O emissions from leguminous tree plantation soils in the humid tropics. Global Biogeochem Cycles 22:GB2028. https://doi.org/10.1029/2007gb002965

    Article  Google Scholar 

  • Baba M, Okazaki M (1998) Soil science and plant nutrition acidification in nitrogen-saturated forested catchment acidification in nitrogen-saturated forested catchment. Soil Sci Plant Nutr 768:513–525. https://doi.org/10.1080/00380768.1998.10414475

    Article  Google Scholar 

  • Bell CW, Fricks BE, Rocca JD, Steinweg JW, McMahon SK, Wallenstein MD (2013) High-throughput fluorometric measurement of potential soil extracellular enzyme activities. J Vis Exp 81:e50961. https://doi.org/10.3791/50961

    Article  CAS  Google Scholar 

  • Bowman WD, Cleveland CC, Halada `L, Hresko J, Baron JS (2008) Negative impact of nitrogen deposition on soil buffering capacity. Nat Geosci 1:767–770

    Article  CAS  Google Scholar 

  • CIFOR (2008) Site management and productivity in tropical plantation forests. In: Nambiar EKS (eds) Proceedings of workshops in Piracicaba (Brazil) 22–26 November 2004 and Bogor (Indonesia) 6–9 November 2006. Bogor, Indonesia: Center for International Forestry Research

  • Cossalter C, Pye-Smith C (2003) Fast-wood forestry: myths and realities. Bogor, Indonesia: The Center for International Forestry Research (CIFOR)

  • Crews TE, Kitayama K, Fownes JH, Fownes JH, Riley RH, Herbert DA, Mueller-D D, Vitousek PM (1995) Changes in soil phosphorus fractions and ecosystem dynamics across a long chronosequence in Hawaii. Ecology 76:1407–1424

    Article  Google Scholar 

  • Dong WY, Zhang XY, Liu XY, Fu XL, Chen FS, Wang HM, Sun XM, Wen XF (2015) Responses of soil microbial communities and enzyme activities to nitrogen and phosphorus additions in Chinese fir plantations of subtropical China. Biogeosciences 12:5537–5546. https://doi.org/10.5194/bg-12-5537-2015

    Article  Google Scholar 

  • Fanin N, Hättenschwiler S, Schimann H, Fromin N (2015) Interactive effects of C, N and P fertilization on soil microbial community structure and function in an Amazonian rain forest. Funct Ecol 29:140–150. https://doi.org/10.1111/1365-2435.12329

    Article  Google Scholar 

  • FAO (2006) World reference base for soil resources 2006. A framework international classification, correlation and communication. Food and Agriculture Organization of The United Nations, Rome

    Google Scholar 

  • Gao JG, Zhao P, Shen WJ, Rao XQ, Hu YT (2017) Physiological homeostasis and morphological plasticity of two tree species subjected to precipitation seasonal distribution changes. Perspect Plant Ecol Evol Syst 25:1–19. https://doi.org/10.1016/j.ppees.2017.01.002

    Article  Google Scholar 

  • Hardiyanto EB, Anshori S, Sulistyono D (2004) Early results of site management in Acacia mangium plantation at PT Musi Hutan Persada, South Sumatra, Indonesia. In: Nambiar, E.K.S. (Editor) Site management and productivity in tropical plantation forests. Proceedings of workshops in Piracicaba (Brazil) 22–26 November 2004 and Bogor (Indonesia) 6–9 November 2006. Bogor, Indonesia: Center for International Forestry. pp 93–108

  • Harwood CE, Nambiar EKS (2014) Productivity of acacia and eucalypt plantations in Southeast Asia. 2. trends and variations. Int For Rev 16:249–260. https://doi.org/10.1505/146554814811724766

    Article  Google Scholar 

  • Kaspari M, Milton N, Harms KE, Wright SJ (2008) Multiple nutrients limit litterfall and decomposition in a tropical forest. Ecol Lett 11:35–43. https://doi.org/10.1111/j.1461-0248.2007.01124.x

    Article  PubMed  Google Scholar 

  • Keenan RJ, Reams GA, Achard F, De Freitas JV, Grainger A, Lindquist E (2015) Dynamics of global forest area: results from the FAO global forest resources assessment 2015. For Ecol Manage 352:9–20. https://doi.org/10.1016/j.foreco.2015.06.014

    Article  Google Scholar 

  • Kitayama K, Aiba SI (2002) Ecosystem structure and productivity of tropical rain forests along altitudinal gradients with contrasting soil phosphorus pools on Mount Kinabalu, Borneo. J Ecol 90:37–51. https://doi.org/10.1046/j.0022-0477.2001.00634.x

    Article  Google Scholar 

  • Konda R, Ohta S, Ishizuka S, Heriyanto J, Wicaksono A (2010) Seasonal changes in the spatial structures of N2O, CO2, and CH4 fluxes from Acacia mangium plantation soils in Indonesia. Soil Biol Biochem 42:1512–1522. https://doi.org/10.1016/j.soilbio.2010.05.022

    Article  CAS  Google Scholar 

  • Lu XK, Mao QG, Mo JM, Gilliam F, Zhou GY, Luo YQ (2015) Divergent responses of soil buffering capacity to long-term n deposition in three typical tropical forests with different land-use history. Environ Sci Technol 49:4072–4080. https://doi.org/10.1021/es5047233

    Article  CAS  PubMed  Google Scholar 

  • Mori T, Ohta S, Ishizuka S, Konda R, Wicaksono A, Heriyanto J (2010) Effects of phosphorus addition on N2O and NO emissions from soils of an Acacia mangium plantation. Soil Sci Plant Nutr 56:782–788. https://doi.org/10.1111/j.1747-0765.2010.00501.x

    Article  CAS  Google Scholar 

  • Mori Ohta S, Ishizuka S, Konda R, Wicaksono A, Heriyanto J, Hardjono A (2013a) Effects of phosphorus and nitrogen addition on heterotrophic respiration in an Acacia mangium plantation soil in South Sumatra, Indonesia. Tropics 22:83–87. https://doi.org/10.1111/j.1747-0765.2010.00501.x

    Article  CAS  Google Scholar 

  • Mori Ohta S, Ishizuka S, Konda R, Wicaksono A, Heriyanto J (2013b) Soil greenhouse gas fluxes and C stocks as affected by phosphorus addition in a newly established Acacia mangium plantation in Indonesia. For Ecol Manage 310:643–651. https://doi.org/10.1016/j.foreco.2013.08.010

    Article  Google Scholar 

  • Mori T, Ohta S, Ishizuka S, Konda R (2014) Phosphorus application reduces N2O emissions from tropical leguminous plantation soil when phosphorus uptake is occurring. Biol Fertil Soils 50:45–51. https://doi.org/10.1007/s00374-013-0824-4

    Article  CAS  Google Scholar 

  • Mori T, Ishizuka S, Konda R, Genroku T, Nakamura R, Kajino H, Wicaksono A, Heriyanto J, Hardjono A, Ohta S (2018) Potassium and magnesium in leaf and top soil affected by triple superphosphate fertilization in an Acacia mangium plantation. J Trop Forest Sci 30:xx–xx

    Google Scholar 

  • Payn T, Carnus JM, Freer-Smith P, Kimberley M, Kolleert W, Liu S (2015) Changes in planted forests and future global implications. For Ecol Manage 352:57–67. https://doi.org/10.1016/j.foreco.2015.06.021

    Article  Google Scholar 

  • Turner BL, Wright SJ (2014) The response of microbial biomass and hydrolytic enzymes to a decade of nitrogen, phosphorus, and potassium addition in a lowland tropical rain forest. Biogeochemistry 117:115–130. https://doi.org/10.1007/s10533-013-9848-y

    Article  CAS  Google Scholar 

  • Uddin MB, Mukul SA, Khan MASA, Hossain MK (2007) Effects of phosphorous fertilizer on seedlings growth and nodulation capabilities of some popular agroforestry tree species of Bangladesh. J For Res 18:283–286. https://doi.org/10.1007/s11676-007-0054-4

    Article  Google Scholar 

  • Uddin MB, Mukul SA, Khan MASA, Hossain MK (2009) Seedling response of three agroforestry tree species to phosphorous fertilizer application in Bangladesh: growth and nodulation capabilities. J For Res 20:45–48. https://doi.org/10.1007/s11676-009-0007-1

    Article  Google Scholar 

  • van Breemen N, Mulder J, Driscoll CT (1983) Acidification and alkalinization of soils. Plant Soil 75:283–308. https://doi.org/10.1007/bf02369968

    Article  Google Scholar 

  • Vitousek PM, Porder S, Houlton BZ, Chadwick O (2010) Terrestrial phosphorus limitation: mechanisms, implications, and nitrogen-phosphorus interactions. Ecol Appl 20:5–15

    Article  Google Scholar 

  • Walker TW, Syers JK (1976) The fate of phosphorus during pedogenesis. Geoderma 15:1–19. https://doi.org/10.1016/0016-7061(76)90066-5

    Article  CAS  Google Scholar 

  • Yamashita N, Ohta S, Hardjono A (2008) Soil changes induced by Acacia mangium plantation establishment: comparison with secondary forest and Imperata cylindrica grassland soils in South Sumatra, Indonesia. For Ecol Manage 254:362–370. https://doi.org/10.1016/j.foreco.2007.08.012

    Article  Google Scholar 

  • Yashiro N, Yamashita N, Ohta S (2012) Effects of nitrogen application to phosphorous forms and acid phosphatase activity (APA) in soils of Acaica mangium plantation and Imperata cylindrica grassland. Japanese J For Environ 54:93–100

    Google Scholar 

  • Zhang W, Zhu XM, Liu L, Fu SL, Chen H, Huang J, Lu XK, Liu Z, Mo JM (2012) Large difference of inhibitive effect of nitrogen deposition on soil methane oxidation between plantations with N-fixing tree species and non- N-fixing tree species. J Geophys Res Atmos 117:G00N16. https://doi.org/10.1029/2012jg002094

    Article  Google Scholar 

  • Zhang W, Zhu XM, Luo YQ, Rafique R, Chen H, Huang J, Mo JM (2014) Responses of nitrous oxide emissions to nitrogen and phosphorus additions in two tropical plantations with N-fixing vs. non-N-fixing tree species. Biogeosciences 11:4941–4951. https://doi.org/10.5194/bg-11-4941-2014

    Article  Google Scholar 

  • Zheng MH, Li DJ, Lu XK, Zhu XM, Zhang W, Huang J, Fu SL, Lu XK, Mo JM (2016) Effects of phosphorus addition with and without nitrogen addition on biological nitrogen fixation in tropical legume and non-legume tree plantations. Biogeochemistry 131:65–76. https://doi.org/10.1007/s10533-016-0265-x

    Article  CAS  Google Scholar 

  • Zhou Z, Wang C, Jin Y (2017) Stoichiometric responses of soil microflora to nutrient additions for two temperate forest soils. Biol Fertil Soils 53:397–406. https://doi.org/10.1007/s00374-017-1188-y

    Article  CAS  Google Scholar 

  • Zhu XM, Chen H, Zhang W, Huang J, Fu SL, Liu ZF, Mo JM (2015) Effects of nitrogen addition on litter decomposition and nutrient release in two tropical plantations with N2-fixing vs. non-N2-fixing tree species. Plant Soil. https://doi.org/10.1007/s11104-015-2676-1

    Article  Google Scholar 

Download references

Acknowledgements

We thank Shengxing Fu and Meifang Hu for their support for the field work. We acknowledge the two anonymous reviewers for providing helpful comments on an initial version of this manuscript. This study was financially supported by National Natural Science Foundation of China (No. 31670488, 41650110484, and 41731176), Grant-in-Aid for JSPS Postdoctoral Fellowships for Research Abroad (28.601), and a Grant from The Sumitomo Foundation (153082).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Wei Zhang.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Project Funding: This study was financially supported by the National Natural Science Foundation of China (No. 31670488, 41650110484, and 41731176), the Natural Science Foundation of Guangdong Provine (No. 2017A030313168), Grant-in-Aid for JSPS Postdoctoral Fellowships for Research Abroad (28.601), and a grant from The Sumitomo Foundation (153082).

The online version is available at http://www.springerlink.com

Corresponding editor: Chai Ruihai.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, S., Mori, T., Mo, J. et al. The responses of carbon- and nitrogen-acquiring enzymes to nitrogen and phosphorus additions in two plantations in southern China. J. For. Res. 31, 1319–1324 (2020). https://doi.org/10.1007/s11676-019-00905-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11676-019-00905-0

Keywords

Navigation