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Functional properties and the oligomeric state of alkyl hydroperoxide reductase subunit F (AhpF) in Pseudomonas aeruginosa

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Abstract

Alkyl hydroperoxide reductase subunit F (AhpF) is a well-known flavoprotein that transfers electrons from pyridine nucleotides to the peroxidase protein AhpC via redox-active disulfide centers to detoxify hydrogen peroxide. However, study of AhpF has historically been limited to particular eubacteria, and the connection between the functional and structural properties of AhpF remains unknown. The present study demonstrates the dual function of Pseudomonas aeruginosa AhpF (PaAhpF) as a reductase and a molecular chaperone. It was observed that the functions of PaAhpF are closely linked with its structural status. The reductase and foldase chaperone function of PaAhpF predominated for its low-molecular-weight (LMW) form, whereas the holdase chaperone function of PaAhpF was found associated with its high-molecular-weight (HMW) complex. Further, the present study also demonstrates the multiple function of PaAhpF in controlling oxidative and heat stresses in P. aeruginosa resistance to oxidative and heat stress.

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Acknowledgments

This project was supported by the Nuclear R&D Program of the Ministry of Science and ICT (MSIT), Republic of Korea.

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Author notes

  1. Sung Hyun Hong and Sudhir Singh contributed equally to this work.

Authors and Affiliations

  1. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup, 56212, South Korea

    Sung Hyun Hong, Sudhir Singh, Bhumi Nath Tripathi, Suvendu Mondal, Chuloh Cho, Shubhpreet Kaur, Jin-Hong Kim, Sungbeom Lee, Hyoung-Woo Bai, Seung Sik Lee & Byung Yeoup Chung

  2. Department of Bioenergy Science and Technology, Chonnam National University, Gwangju, 61186, South Korea

    Sung Hyun Hong & Hyeun-Jong Bae

  3. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400085, India

    Sudhir Singh & Suvendu Mondal

  4. Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, 484887, India

    Bhumi Nath Tripathi

  5. Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, 24341, South Korea

    Sangmin Lee & Hyun Suk Jung

  6. Department of Radiation Science and Technology, University of Science and Technology, Daejeon, 34113, South Korea

    Shubhpreet Kaur, Sungbeom Lee, Hyoung-Woo Bai & Seung Sik Lee

  7. Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju, 52828, South Korea

    Sang Yeol Lee

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Hong, S.H., Singh, S., Tripathi, B.N. et al. Functional properties and the oligomeric state of alkyl hydroperoxide reductase subunit F (AhpF) in Pseudomonas aeruginosa. Protoplasma 257, 807–817 (2020). https://doi.org/10.1007/s00709-019-01465-0

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