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Effect of Escherichia coli on phospholipid monolayers: surface tensiometry and Brewster angle microscopy measurements

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Abstract

The effect of Escherichia coli (E. coli) cells on two phospholipids [dipalmitoyl phosphatidylcholine (DPPC) and dimyristoyl phosphatidylcholine (DMPC)] monolayers at the surface of a 1.5 wt% NaCl salt solution has been investigated using surface tension measurement and Brewster angle microscopy. The results showed that a DPPC monolayer that has an elastic structure was changed in morphology by interaction with E. coli cells, whereas a DMPC monolayer that has an expandable structure did not change in morphology. In particular, the morphology changed significantly around the liquid-expanded (LE)–liquid-condensed (LC) phase transition point for the DPPC monolayer. It was found that the LE–LC phase transition range in a DPPC monolayer was sensitive to influence from the outside of the monolayer such as the action of E. coli cells. Such a monolayer has the potential for application as a membrane sensor for detecting a small amount of bacteria in a short time.

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References

  • Aroti A, Leontidis E, Maltseva E, Brezesinski G (2004) Effects of hofmeister anions on DPPC langmuir monolayers at the air–water interface. J Phys Chem B 108:15238–15245

    Article  CAS  Google Scholar 

  • Birdi KS, Vu DT (1994) Structures of collapsed lipid monolayers investigated as Langmuir–Blodgett films by atomic force microscopy. Langmuir 10:623–625

    Article  CAS  Google Scholar 

  • Gellman SH (1997) Introduction: molecular recognition. Chem Rev 97:1231–1232

    Article  CAS  Google Scholar 

  • Gennis RG (1990) Biomembrane, molecular structure and function. Springer, New York

    Google Scholar 

  • Ha TA, Pham T (2006) Study of Salmonella, Campylobacter, and Escherichia coli contamination in raw food available in factories, schools, and hospital canteens in Hanoi, Vietnam. Ann NY Acad Sci 1081:262–265

    Article  Google Scholar 

  • Hadicke A, Blume A (2016) Binding of the cationic peptide (KL)4K to lipid monolayers at the air–water interface: effect of lipid headgroup charge, acyl chain length, and acyl chain saturation. J Phys Chem B 120:3880–3887

    Article  Google Scholar 

  • Jang J, Hur HG, Sadowsky MJ, Byappanahalli MN, Yan T, Ishii S (2017) Environmental Escherichia coli: ecology and public health implications—a review. J. Appl Microbiol 123:570–581

    Article  CAS  Google Scholar 

  • Kabalnov A, Olsson U, Wennerstrom H (1995) Salt Effects on nonionic microemulsions are driven by adsorption/depletion at the surfactant monolayer. J Phys Chem 99:6220–6230

    Article  CAS  Google Scholar 

  • Kato T, Matsumoto N, Kawano M, Suzuki N, Iriyama K (1994) Transition to three-dimensional structures in monolayers of a series of long-chain acids on the water surface by compression studied by transmission electron microscopy and atomic force microscopy. Thin Solid Films 242:223–228

    Article  CAS  Google Scholar 

  • Komada M, Shibata O, Nakamura S, Lee S, Sugihara G (2004) A monolayer study on three binary mixed systems of dipalmitoyl phosphatidylcholine with cholesterol, cholestanol and stigmasterol. Col Surf B 33:211–226

    Article  Google Scholar 

  • Mangiarotti A, Caruso B, Willke N (2014) Phase coexistence in films composed of DLPC and DPPC: a comparison between different model membrane systems. Biochem Biophys Acta 1838:1823–1831

    Article  CAS  Google Scholar 

  • Meer VM, Voelker DR, Feigenson GW (2008) Membrane lipids: where they are and how they behave. Nat Rev Mol Cell Biol 9:112–124

    Article  Google Scholar 

  • Mingotaud AF, Mingotaud C, Patterson LK (1993) Handbook of monolayers, vol 1. Academic Press, San Diego

    Google Scholar 

  • Nelson DL, Cox MM (2017) Lehninger principles of biochemistry, 7th edn. WH Freeman, New York

    Google Scholar 

  • Ohe C, Sasaki T, Noi M, Goto Y, Itoh K (2007) Sum frequency generation spectroscopic study of the condensation effect of cholesterol on a lipid monolayer. Anal Bioanal Chem 388:73–79

    Article  CAS  Google Scholar 

  • Okamura Y, Nakamura M (1999) NMR study directly determining drug delivery sites in phospholipid bilayer membranes. J Phys Chem B 103:3505–3509

    Article  CAS  Google Scholar 

  • Scheie PO, Rehberg R (1972) Response of Escherichia coli B/r to high concentrations of sucrose in a nutrient medium. J Bacteriol 109:229–235

    Article  CAS  Google Scholar 

  • Schramm LL, Stasiuk EN, Marangoni DG (2003) Surfactants and their applications. Annu Rep Prog Chem Sect C Phys Chem 99:3–48

    Article  CAS  Google Scholar 

  • Spector AA, Yorek MA (1985) Membrane lipid composition and cellular function. J Lipid Res 26:1015–1035

    CAS  PubMed  Google Scholar 

  • Suezaki Y (2007) Physics of lipid membrane. Kyusyu Univ Press, Fukuoka

    Google Scholar 

  • Takahashi H, Yasue T, Ohki K, Hatta I (1996) Structure and phase behaviour of dimyristoylphosphatidic acid/poly(L-lysine) systems. Mol Memb Biol 13:233–240

    Article  CAS  Google Scholar 

  • Yamamoto Y, Taga K (2016) Lipid monolayer and interaction with anesthetics, Encyclopedia of biocolloid and biointerface science, vol 1. Wiley, Hoboken

    Google Scholar 

  • Yamamoto Y, Yokoyama T, Yoshida D, Mori H, Sekiguchi K, Shimoaki T, Yoshino A, Taga K, Shervani Z, Yamamoto M (2015) Interaction between phospholipid monolayers (DPPC and DMPC) and anesthetic isoflurane observed by quartz crystal oscillator. J Biophys Chem 6:42–53

    Article  CAS  Google Scholar 

  • Yeagle PL (2016) The membranes of cells, 3rd edn. Academic Press, New York

    Google Scholar 

  • Yokoyama T, Yoshida D, Mori H, Okabe M, Shervani Z, Taga K, Yamamoto Y, Sumino A, Dewa T, Nango M, Yamamoto M (2016) Morphological observation of specific condensation effect of cholesterol on dipalmitoyl phosphatidyl choline (DPPC) monolayer by dropping method. J Biophys Chem 7:98–109

    Article  CAS  Google Scholar 

  • Yoshida D, Yokoyama T, Shimoaki T, Tomita T, Yoshida T, Yamamoto Y, Taga K, Sumino A, Dewa T, Nango M, Yamamoto M, Shervani Z (2013) Morphology observation of dipalmitoyl phosphatidyl choline (DPPC) monolayer on water surface by dropping method. J Biophys Chem 4:115–121

    Article  Google Scholar 

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Funding

This work was supported by a Grant-in-Aid from the Ministry of Science, Education, Sports, and Culture (nos. 21750143 and 24550154), “The Knowledge Hub” of AICHI, The Priority Research Project.

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Authors and Affiliations

  1. Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi, 466-8555, Japan

    Masaya Okabe, Keijiro Taga, Akihiro Yoshino & Yasushi Yamamoto

  2. Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan

    Akiko Taneda, Suguru Shinoda & Shin Kanamasa

  3. Division of Nanomaterials Production, Food and Energy Security Research and Product Center, Aoba-ku, Sendai, Miyagi, 980-0871, Japan

    Zameer Shervani

Authors
  1. Masaya Okabe
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  2. Keijiro Taga
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  3. Akihiro Yoshino
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  4. Yasushi Yamamoto
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  5. Akiko Taneda
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  6. Suguru Shinoda
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  7. Shin Kanamasa
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  8. Zameer Shervani
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Correspondence to Yasushi Yamamoto or Zameer Shervani.

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Okabe, M., Taga, K., Yoshino, A. et al. Effect of Escherichia coli on phospholipid monolayers: surface tensiometry and Brewster angle microscopy measurements. Eur Biophys J 49, 71–84 (2020). https://doi.org/10.1007/s00249-019-01413-z

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  • DOI: https://doi.org/10.1007/s00249-019-01413-z

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