Abstract
Picrorhiza kurroa is a medicinal perennial herb of economic importance due to its hepatoprotective properties mainly accounted by picroside I and picroside II. To fulfill the current demand of the market indiscriminate collection from its natural habitat pose a great threat to this endangered species. To strategize the conservation of natural populations, a set of 20 highly informative novel genic SSR markers were identified. The utility of these makers was successfully tested for the genetic diversity characterization of P. kurroa populations (n = 28) from three geographical locations. These markers produced 136 alleles (average of 6.8) with mean observed heterozygosity, expected heterozygosity, Shannon’s information index, and PIC value of 0.971, 0.798, 1.681, and 0.737 respectively, revealing a higher extent of genetic diversity in P. kurroa. Further, clustering of all the individuals according to their geographical locations indicates at a spatial population structure in P. kurroa. The current study suggests that informative SSR makers identified here can be potentially used for diversity characterization targeting wider geographical locations for selection of elite/quality genotypes for commercial cultivation and genetic rescue of this endangered species.
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Abbreviations
- SSRs:
-
Simple sequence repeats
- PKGMS:
-
Picrorhiza kurroa Genic MicroSatellite
- PIC:
-
Polymorphic information content
- PCoA:
-
Principal coordinate analysis
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Acknowledgements
The research work was funded by MLP-0071 and Phyto-pharmaceutical Mission (HCP0010) of Council of Scientific and Industrial Research (CSIR), Government of India. We also greatly acknowledge The Director, CSIR-IHBT for providing necessary facilities for conducting research. This is IHBT communication no. 4282.
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Supplementary Fig.
1: (a) Characteristics and number of SSRs (b) Number of different nucleotide repeats identified in P. kurroa transcriptome. (TIFF 4168 kb)
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Singh, P., Sharma, R.K. Development of informative genic SSR markers for genetic diversity analysis of Picrorhiza kurroa. J. Plant Biochem. Biotechnol. 29, 144–148 (2020). https://doi.org/10.1007/s13562-019-00515-1
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DOI: https://doi.org/10.1007/s13562-019-00515-1