Abstract
The abdomens of honey bee queens and semen from drone bees were analyzed by visible and near-infrared spectroscopy. Mated honey bee queens could be distinguished from virgin queens by their absorption spectra with 100% accuracy. Spectra of semen showed that classifications of queens were likely influenced by the presence or absence of semen in the queen spermathecae. However, physiological or morphological changes that occur in the queens after mating probably influenced the classifications also.
Zusammenfassung
Im Allgemeinen können die inneren Organe der Honigbienen nicht untersucht werden, ohne die Bienen zuvor zu töten. Eine schnelle und nicht invasive Methode zum Nachweis von Sperma in der Spermatheka der Bienenkönigin wäre aber äußerst nützlich. Die VIS-NIR-Spektroskopie (parallele Spektroskopie im sichtbaren Wellenlängenbereich und im Nahen Infrarot) wurde bereits erfolgreich bei anderen Untersuchungen von Insektengeweben eingesetzt; hier wurde sie dazu benutzt, unbegattete Königinnen, begattete Königinnen und das zugehörige Drohnensperma zu identifizieren. Wir untersuchten 52 begattete und 52 unbegattete Königinnen, indem wir den Strahl des Spektrometers auf den Abschnitt des Königinnenabdomens richteten, in dem sich die Spermatheka befindet. Dabei konnten begattete von unbegatteten Königinnen zu 100% unterschieden werden (Abb. 1 und Abb. 2). Die Unterschiede in den Spektren könnten durch das Sperma in der Spermatheka der begatteten Königinnen hervorgerufen worden sein (Abb. 3). Allerdings scheinen auch physiologische und morphologische Veränderungen, die bei der Königin nach der Paarung auftreten, eine wichtige Rolle bei den Unterschieden zwischen den Spektren zu spielen.
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Webster, T.C., Dowell, F.E., Maghirang, E.B. et al. Visible and near-infrared spectroscopy detects queen honey bee insemination. Apidologie 40, 565–569 (2009). https://doi.org/10.1051/apido/2009038
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DOI: https://doi.org/10.1051/apido/2009038