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Autophagy in Hepatocellular Carcinoma-29 after Single or Combined Administration of Lithium Carbonate and Rapamycin

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Abstract

The role of autophagy in the development and progression of hepatocellular carcinoma (HCC) is ambiguous and still little known. Autophagy stimulation may be of exceptional interest in antitumor pharmacotherapy of HCC. Rapamycin and lithium are typical inducers of autophagy. The aim of this study was to compare the level of autophagy in hepatocellular carcinoma-29 (HCC-29) cells after single and combined administration of lithium carbonate and rapamycin. Autolysosomes formation and significant increase of LC3 beta (+)– and LAMP1 (+)– autophagic structures were revealed in HCC-29 cells after lithium carbonate and rapamycin coadministration by transmission electron microscopy and immunofluorescence analysis. Using this combination of drugs may be a promising strategy for HCC chemotherapy, since it will allow the integration of various cellular signaling pathways that regulate autophagy and apoptosis in tumor cells.

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Funding

This work was supported with financing of the Novosibirsk Scientific-Research Institute of Clinical and Experimental Lymphology as part of a state order, no. 0324-2019-0045.

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Authors and Affiliations

  1. Research Institute of Clinical and Experimental Lymphology, Branch of the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630060, Novosibirsk, Russia

    Iu. S. Taskaeva, N. P. Bgatova & A. O. Solovieva

  2. BNCT Laboratory of Novosibirsk State University, 630090, Novosibirsk, Russia

    Iu. S. Taskaeva

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  1. Iu. S. Taskaeva
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  2. N. P. Bgatova
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  3. A. O. Solovieva
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Correspondence to Iu. S. Taskaeva.

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COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

AUTHOR CONTRIBUTIONS

The concept and design of the study, as well as the analysis and interpretation of the data, were carried out by Iu.S. Taskaeva and N.P. Bgatova, collection and processing of material were done by Iu.S. Taskaeva and A.O. Solovieva, and statistical data processing and paper writing were done by Iu.S. Taskaeva.

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Translated by I. Fridlyanskaya

Abbreviations: HCC—hepatocellular carcinoma, HCC-29—hepatocellular carcinoma-29, AKT—protein kinase B, GSK-3β—glycogen synthase kinase-3β, IMPase—inosytol monophosphatase, IP3—inositol-1,4,5-triphosphate, IP3R–IP3 receptor, mTOR—mammalian target for rapamycin, PI3K—phosphatidylinositol 3-kinase, TSC 1/2—tuberous sclerosis complex 1/2.

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Taskaeva, I.S., Bgatova, N.P. & Solovieva, A.O. Autophagy in Hepatocellular Carcinoma-29 after Single or Combined Administration of Lithium Carbonate and Rapamycin. Cell Tiss. Biol. 13, 353–359 (2019). https://doi.org/10.1134/S1990519X19050079

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