Abstract
Lysosomal-associated membrane protein 1 (LAMP1) mainly localizes to lysosomes and late endosomes. We herein investigated the intracellular localization of lysosomal membrane proteins in five mammalian cultured cell lines. Rat LAMP1 fused to enhanced green fluorescent protein (EGFP) mostly accumulated at a particular cytoplasmic area and barely co-localized with LysoTracker® Red DND-99 in golden hamster kidney BHK-21 cells and Chinese hamster ovary CHO-K1 cells. Golden hamster, Chinese hamster, and human LAMP1-EGFP showed a similar intracellular distribution to rat LAMP1-EGFP in BHK-21 cells. Endogenous LAMP1 was also detected in a perinuclear area in BHK-21 cells and CHO-K1 cells, and co-localized with rat CD63-EGFP in BHK-21 cells. Moreover, rat LAMP1-DsRed-Monomer co-localized well with the human trans-Golgi network protein 2-EGFP in BHK-21 cells. These results reveal that LAMP1 predominantly localizes to the trans-Golgi network in BHK-21 cells.
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07 July 2021
A Correction to this paper has been published: https://doi.org/10.1007/s00418-021-02010-y
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Acknowledgements
We are very grateful to Drs. Della Reynolds, Craig W. Reynolds, Hideo Yagita, and Rafick-Pierre Sékaly for their kind gifts. We also thank Yoko Yaji and Hisashi Yamada for their contribution to this study. This work was partly supported by JSPS KAKENHI Grant numbers 22658036, 15K14920, and 19H02885 (to T.K.).
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Baba, K., Kuwada, S., Nakao, A. et al. Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines. Histochem Cell Biol 153, 199–213 (2020). https://doi.org/10.1007/s00418-019-01842-z
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DOI: https://doi.org/10.1007/s00418-019-01842-z