Abstract
Catechol is a highly toxic compound that is also a key intermediate in biodegradation pathways of various aromatic compounds. In this paper, a new screening method for isolation of microorganisms with the potential for catechol biodegradation is reported. The method described is based on the ability of catechol to form a color complex with Fe3+ ions. For this purpose, basal medium that is widely used for classification of microorganisms by carbon requirements was used. Specifically, minimal Bushnell Haas medium plates supplemented with catechol at 500 mg l−1 concentration were used for both yeast and bacteria cultivation. After appearance of microorganism colonies on the growth medium, each plate was flooded with 7.0 ml of 5% (w/v) of FeCl3 and incubated for a period of 5 min at room temperature. Flooding with this solution led to the formation of a green color from Fe3+-catechol complexes in the growth medium and an appearance of clear zones around colonies of microorganisms that utilize catechol as a sole source of carbon. Based on this, the presented method could be useful as a screening step for microorganisms with a potential for catechol biodegradation.
Article Highlights
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A new screening method for isolation of microorganisms with the potential for catechol biodegradation has been proposed.
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The presented method is based on the ability of catechol to form a color complex of catechol and Fe3+ ions and appearance of clear zone around colonies of microorganisms which utilize catechol.
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Described method is simple, not labor-intensive, does not require a long period of time to achieve unmistakable results.
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This method may be also used as a starting point for screening microorganisms with the potential for other aromatic hydrocarbons degradation, in cases when catechol acts like a key intermediate in their biodegradation pathway.
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References
Al-Abadleh HA (2015) Review of the bulk and surface chemistry of iron in atmospherically relevant systems containing humic-like substances. RSC Adv 5:45785–45811. https://doi.org/10.1039/C5RA03132J
Aleksieva Z, Ivanova D, Godjevargova T, Atanasov B (2002) Degradation of some phenol derivatives by Trichosporon cutaneum R57. Process Biochem 37:1215–1219. https://doi.org/10.1016/S0032-9592(01)00336-3
Bramhachari PV, Reddy DRS, Kotresha D (2016) Biodegradation of catechol by free and immobilized cells of Achromobacter xylosoxidans strain 15DKVB isolated from paper and pulp industrial effluents. Biocatal Agric Biotechnol 7:36–44. https://doi.org/10.1016/j.bcab.2016.05.003
Bull C, Ballou DP (1981) Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometry. J Biol Chem 256:12673–12680
Di Gioia D, Barberio C, Spagnesi S, Marchetti L, Fava F (2002) Characterization of four olive-mill-wastewater indigenous bacterial strains capable of aerobically degrading hydroxylated and methoxylated monocyclic aromatic compounds. Arch Microbiol 178:208–217. https://doi.org/10.1007/s00203-002-0445-z
Farahani M, Mirbagheri SA, Javid AH, Karbassi AR, Khorasani N, Nouri J (2010) Biodegradation and leaching of polycyclic aromatic hydrocarbons in soil column. J Food Agric Envrion 8:870–875. https://doi.org/10.1234/4.2010.1867
Filipowicz N, Momotko M, Boczkaj G, Pawlikowski T, Wanarska M, Cieśliński H (2017) Isolation and characterization of phenol-degrading psychrotolerant yeasts. Water Air Soil Pollut 228:210. https://doi.org/10.1007/s11270-017-3391-8
Hemalatha S, VeeraManikandan P (2011) Characterization of aromatic hydrocarbon rading bacteria from petroleum contaminated sites. J Environ Prot 2:243–254. https://doi.org/10.4236/jep.2011.23028
IARC (1999) Re-evaluation of Some Organic Chemicals, Hydrazine and Hydrogen Peroxide. IARC monographs on the evaluation of cancerogenic risk to humans. 71:433–435
Jayanthi R, Hemashenpagam N (2015) Optimization of BH medium for efficient biodegradation of benzene, toluene and xylene by a Bacillus cereus. Int J Curr Microbiol Appl Sci 4:807–881
Kafilzadeh F, Farhangdoost M-S, Tahery Y (2010) Isolation and identification of phenol degrading bacteria from Lake Parishan and their growth kinetic assay. Afr J Biotechnol 9:6721–6726. https://doi.org/10.5897/AJB10.665
Kim JM, Le NT, Chung BS, Park JH, Bae J-W, Madsen EL, Jeon CO (2008) Influence of soil components on the biodegradation of benzene, toulene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudomonas spadix BD-a59. AEM 74:7313–7320
Kumar A, Kumar S, Kumar S (2005) Biodegradation kinetics of phenol and catechol using Pseudomonas putida MTCC 1194. Biochem Eng J 22:151–159. https://doi.org/10.1016/j.bej.2004.09.006
Lee BP (2016) Mussel adhesive-inspired polymers. In: Bruns N, Kilbinger AFM (eds) Bio-inspired polymers. Royal Society of Chemistry, Cambridge, pp 322–353. https://doi.org/10.1039/9781782626664-00322
Meng H, Liu Y, Cencer MM, Lee BP (2015) Adhesives and coatings inspired by mussel adhesive proteins. In: Bianco-Peled H, Davidovich-Pinhas M (eds) Bioadhesion and biomimetics. Pan Stanford, Singapore, pp 131–166
Middelhoven WJ, de Jong IM, de Winter M (1991) Arxula adeninivorans, a yeast assimilating many nitrogenous and aromatic compounds. Antonie Van Leeuwenhoek 59:129–137. https://doi.org/10.1007/BF00445657
Mrozik A, Piotrowska-Seget Z, Labuzek S (2007) FAME profiles in Pseudomonas vesicularis during catechol and phenol degradation in the presence of glucose as an additional carbon source. Pol J Microbiol 56:157–164
Nair C, Jayachandran K, Shashidhar S (2002) Biodegradation of phenol. Afr J Biotechnol 7:4951–4958. https://doi.org/10.1046/j.1462-2920.2001.00176.x
Rigo M, Alegre RM, Bezerra JRMV et al (2010) Catechol biodegradation kinetics using Candida parapsilopsis. Braz Arch Biol Technol 53:481–486. https://doi.org/10.1590/S1516-89132010000200029
Schweigert N, Zehnder AJB, Eggen RIL (2001) Chemical properties of catechols and their molecular modes of toxic action in cells, from microorganisms to mammals. Minireview. Environ Microbiol 3:81–91. https://doi.org/10.1046/j.1462-2920.2001.00176.x
Shishir TA, Mahbub N, Kamal NE (2019) Review on bioremediation: a tool to resurrect the polluted rivers. Pollution 5:555–568. https://doi.org/10.22059/poll.2019.272339.558
Soudi MR, Kolahchi N (2011) Bioremediation potential of phenol degrading bacterium, Rhodococcus erythropolis SKO-1. Prog Biol Sci 1:31–70. https://doi.org/10.22059/PBS.2011.22457
Stanchev V, Stoilova I, Krastanov A (2008) Biodegradation dynamics of high catechol concentrations by Aspergillus awamori. J Hazard Mater 154:396–402. https://doi.org/10.1016/j.jhazmat.2007.10.038
Tewari L, Malviya P (2002) Biodegradation of catechol by fluorescent Pseudomonas for sustainable environment. J Sci Ind Res India 61:70–74
Thormann MN, Rice AV, Beilman DW (2007) Yeasts in peatlands: a review of richness and roles in peat decomposition. Wetlands 27:761–773. https://doi.org/10.1672/0277-5212(2007)27%5b761:YIPARO%5d2.0.CO
Tsai S-C, Tsai L-D, Li Y-K (2005) An isolated Candida albicans TL3 capable of degrading phenol at large concentration. Biosci Biotechnol Biochem 69:2358–2367. https://doi.org/10.1271/bbb.69.2358
von Nussbaum F, Spiteller P, Rüth M, Steglich W, Wanner G, Gamblin B et al (1998) An iron(III)-catechol complex as a mushroom pigment. Angew Chemie Int Ed 37:3292–3295. https://doi.org/10.1002/(SICI)1521-3773(19981217)37:23%3c3292:AID-ANIE3292%3e3.0.CO;2-N
Wang J, Ma X, Liu S, Sun P, Fan P, Xia C (2012) Biodegradation of phenol and 4-chlorophenol by Candida tropicalis W1. Proc Environ Sci 16:299–303. https://doi.org/10.1016/j.proenv.2012.10.042
Zeyaullah M, Ahmad R, Naseem A, Islam B, Hasan HMI, Abdelkafe AS et al (2009) Catechol biodegradation by Pseudomonas strain: a critical analysis. Int J Chem Sci 7:2211–2221
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Filipowicz, N., Cieśliński, H. A Rapid and Simple Method for Screening Microorganisms with a Potential for Catechol Biodegradation. Int J Environ Res 14, 87–92 (2020). https://doi.org/10.1007/s41742-019-00239-z
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DOI: https://doi.org/10.1007/s41742-019-00239-z