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Automated Platform for Rapid and Reproducible Sample Preparations in Point-of-Care(POC) Molecular Diagnostics

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Abstract

Fully automated system for sample preparation in molecular diagnostics was developed. The small cartridge with multiple chambers and a rotating valve was designed for automated processes such as extraction, separation and purifications during the DNA sample preparation. The automated processes such as injection, mixing, capturing and valve on/off motions were controlled by an affordable, motorized control system. We demonstrated the automated DNA extraction of pathogenic bacteria using this system. The deviations in the purity and real-time polymerase chain reaction (PCR) detection of the automated DNA extraction samples were clearly decreased. The device provided rapid, reliable, and reproducible sample preparation in comparison with manual DNA extraction. This automated platform can be utilized in emergent point-of-care testing of infectious disease and foodborne bacteria.

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Acknowledgements

This work partially supported by SC1290 & NK217F projects of Korea Institute of Machinery and Materials under the auspices of the Ministry of Science, ICT, and Future Planning, Korea. In addition, this work partially supported by by National Research Foundation of Korea (NRF 2016R1C1B2011729).

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Authors and Affiliations

  1. Department of Medical Devices, Korea Institute of Machinery and Materials (KIMM), Daegu, 42994, Korea

    Dongkyu Lee, Ohwon Kwon, Kang-Ho Lee, Jongsu Yun, Changwon kim & Joonho Seo

  2. School of Mechanical Engineering, Kyunpook National Univiersity, Daegu, 41566, Korea

    Jeawan Koo & Gyu Man Kim

Authors
  1. Dongkyu Lee
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  2. Ohwon Kwon
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  3. Kang-Ho Lee
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  4. Jongsu Yun
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  5. Changwon kim
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  6. Joonho Seo
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  7. Jeawan Koo
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  8. Gyu Man Kim
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Correspondence to Ohwon Kwon.

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Lee, D., Kwon, O., Lee, KH. et al. Automated Platform for Rapid and Reproducible Sample Preparations in Point-of-Care(POC) Molecular Diagnostics. BioChip J 13, 288–293 (2019). https://doi.org/10.1007/s13206-019-3304-6

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  • DOI: https://doi.org/10.1007/s13206-019-3304-6

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