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Elevated Levels of Serum Exosomes in Patients with Major Depressive Disorder

  • CLINICAL NEUROCHEMISTRY
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Abstract—

Exosomes are small (30–100 nm) vesicles of the endocytic membrane that may be found in the endosome system of multivesicular organs and are released after fusion of multivesicular bodies with the plasma membrane. Exosomes function as carriers for specific loads and are stable in biological fluids; hence, exosomes are considered as potential biomarkers of different diseases. The degree of involvement and functional significance of exosomes in pathogenesis of depression have been poorly studied. The development of diagnostic methods for depressive disorders based on the analysis of circulating exosomes is very promising, despite the methodological complications related to their isolation and identification. The purpose of this study was to isolate exosomes from the blood serum of patients with major depressive disorder (MDD) and compare them with healthy volunteers using different methods of evaluation. The concentration of serum exosomes assessed using the methods of dynamic light scattering, nanoparticle tracking analysis, and the enzyme-linked immunoassay was statistically elevated in patients with MDD compared to healthy volunteers. The possible connection between the increased level of serum exosomes with the cell-mediated immune activation that is seen in depression is discussed.

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ACKNOWLEDGMENTS

The authors appreciate the support of the representatives of Malvern Panalytical Ltd in the Russian Federation S.L. Vasin and E.O. Duplyakin for their help in the development of optic devices for registration of exosomes.

Funding

The study was supported by the Russian Foundation for Basic Research, grant no. 17-04-01079-a.

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Authors and Affiliations

  1. Moscow Research and Clinical Center for Neuropsychiatry, ul. Donskaya 43, 115419, Moscow, Russia

    A. A. Yakovlev, T. A. Druzhkova, R. V. Nikolaev, V. E. Kuznetsova, A. B. Guekht & N. V. Gulyaeva

  2. Institute of Higher Nervous Activity and Neurophysiology, Moscow, Russia

    A. A. Yakovlev & N. V. Gulyaeva

  3. Institute of Medicine, People’s Friendship University of Russia, Moscow, Russia

    S. K. Gruzdev

Authors
  1. A. A. Yakovlev
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  2. T. A. Druzhkova
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  3. R. V. Nikolaev
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  4. V. E. Kuznetsova
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  5. S. K. Gruzdev
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  6. A. B. Guekht
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  7. N. V. Gulyaeva
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Correspondence to T. A. Druzhkova.

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Conflict of interest. None of the authors had conflict of interest to declare.

Ethical approval. All procedures performed in studies involving human participants were conducted in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Yakovlev, A.A., Druzhkova, T.A., Nikolaev, R.V. et al. Elevated Levels of Serum Exosomes in Patients with Major Depressive Disorder. Neurochem. J. 13, 385–390 (2019). https://doi.org/10.1134/S1819712419040044

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  • DOI: https://doi.org/10.1134/S1819712419040044

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