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Genetic mapping of ionomic quantitative trait loci in rice grain and straw reveals OsMOT1;1 as the putative causal gene for a molybdenum QTL qMo8

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Abstract

Rice is a major dietary source of essential mineral nutrients and toxic elements (aka ionome) for humans. However, the genetic basis underlying the variation in ionome is still largely unknown. Here, we mapped 51 and 61 quantitative trait loci (QTLs) controlling the concentrations of 13 and 15 elements in rice (Oryza sativa L.) grain and straw, respectively, using a recombinant inbred lines (RILs) that were grown at three different field sites in 3 years. Several QTLs were repeatedly detected in both grain and straw or in multiple years; the resulting 87 unique QTLs with 17 of them (20%) were co-localized with previously reported corresponding QTLs and 70 were novel ionomic QTLs. At least, 14 genomic clusters that controlled the concentrations of multiple elements were identified. Furthermore, we identified a molybdate transporter gene OsMOT1;1 as the putative causal gene for a QTL controlling molybdenum concentration in both straw and grain. QTL analyses based on the concentrations of multiple elements in both grain and straw of RIL population grown in three field sites in 3 years allow us to identify tissue common QTLs and reproducible QTLs that were validated in multiple years. The identification of ionomic QTLs will be useful in revealing the molecular mechanisms underlying the accumulation of elements in rice and providing the opportunity to reduce the accumulation of toxic elements and enrich the accumulation of beneficial elements in rice grain.

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Abbreviations

As:

Arsenic

B:

Boron

Ca:

Calcium

CIM:

Composite interval mapping

Cd:

Cadmium

cM:

Centimorgan

Co:

Cobalt

Chr:

Chromosome

Cu:

Copper

HD:

Heading date

ICP-MS:

Inductively coupled plasma mass spectrometry

K:

Potassium

LOD:

Logarithm of the odds

Mg:

Magnesium

Mn:

Manganese

Mo:

Molybdenum

Na:

Sodium

Ni:

Nickel

P:

Phosphorus

Pb:

Lead

QTL:

Quantitative trait locus

RIL:

Recombinant inbred line

Zn:

Zinc

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Funding

The work was supported by the National Key Research and Development Program of China (Grant No. 2018YFD0800206), the National Natural Science Foundation of China (Grant No. 31772382, 31520103914), the Innovative Research Team Development Plan of the Ministry of Education of China (Grant No. IRT_17R56), the Fundamental Research Funds for the Central Universities (Grant No. KYT201802) and the Natural Science Foundation of Jiangsu Province for Distinguished Young Scholars (Grant No. KB20180023).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Chengcheng Wang, Zhong Tang, Zhu Tang, Xin-Yuan Huang & Fang-Jie Zhao

  2. State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, National Rice Research Institute China, Hangzhou, 310006, China

    Jie-Yun Zhuang

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  1. Chengcheng Wang
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  2. Zhong Tang
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  3. Jie-Yun Zhuang
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  4. Zhu Tang
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  5. Xin-Yuan Huang
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  6. Fang-Jie Zhao
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Contributions

F-JZ and X-YH conceived and designed research, CW, Zhong T and Zhu T conducted experiments, J-YZ provided the RIL population, and X-YH, F-JZ and CW wrote the manuscript with contribution from J-YZ. All authors read and approved the manuscript.

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Correspondence to Xin-Yuan Huang.

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Wang, C., Tang, Z., Zhuang, JY. et al. Genetic mapping of ionomic quantitative trait loci in rice grain and straw reveals OsMOT1;1 as the putative causal gene for a molybdenum QTL qMo8. Mol Genet Genomics 295, 391–407 (2020). https://doi.org/10.1007/s00438-019-01632-1

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