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Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties

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Abstract

Efficient expression of the man1 gene for Aspergillus aculeatus β-1,4-mannanase was demonstrated in Pichia pastoris yeast for the first time, and industrially important characteristics of the synthesized enzyme were confirmed. β-Mannanases are industrial enzymes that can be used, in particular, in the feed industry. The most important requirements for feed enzymes are a broad pH range, thermal stability, and high specific activity. The obtained data indicate that the man1 gene from A. aculeatus is potentially useful for the construction of industrial mannanase producers based on Pichia pastoris yeast.

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Funding

The work was completed with the financial support of State Project no. 595-00004-18 PR and the use of GosNIIgenetika, Kurchatov Institute NRC (Moscow, Russia).

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Authors and Affiliations

  1. State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GosNIIgenetika, Kurchatov Institute NRC), 117545, Moscow, Russia

    M. G. Tarutina, M. N. Lazareva, E. I. Semenko & S. P. Sineoky

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  1. M. G. Tarutina
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  2. M. N. Lazareva
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  3. E. I. Semenko
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  4. S. P. Sineoky
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Correspondence to M. G. Tarutina.

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This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by I. Gordon

Abbreviations: BSA, bovine serum albumin; CL, culture liquid; DNS, dinitrosalicylic acid; Km, Michaelis constant; LB medium, lysogeny broth medium; LBG, locust bean gum; NPS, nonstarch polysaccharide; OD540, optical density at wavelength of 540 nm; PAGE, polyacrylamide gel electrophoresis; Vmax, maximum rate of enzymatic reaction; YNB medium, yeast nitrogen base medium; YP medium, medium containing yeast extract and peptone.

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Tarutina, M.G., Lazareva, M.N., Semenko, E.I. et al. Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties. Appl Biochem Microbiol 55, 877–881 (2019). https://doi.org/10.1134/S0003683819090084

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  • DOI: https://doi.org/10.1134/S0003683819090084

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