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Isolation and characterization of 40 SNP in largemouth bass (Micropterus salmoides)

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Abstract

The largemouth bass Micropterus salmoides was introduced into Guandong province of China in 1983, and is now one of the China’s primary cultured fish species. After a long period of farming, the populations are facing the stringent challenges of germplasm resources degraded, and genetic improvement is urgently needed. Thus, developing sufficient molecular markers for the conservation and molecular-aided selection of largemouth bass is urgent. In this study, we implemented restriction site associated DNA sequencing (RAD-seq) to develop single nucleotide polymorphism (SNP) markers. In total, 40 novel SNP markers were found to be polymorphic and showed bi-allelic in 49 individuals of largemouth bass. Those novel SNP markers may be useful for further genetic improvement of largemouth bass.

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Acknowledgements

This work was supported by the Basic Research Business Fees of Chinese Academy of Fishery Sciences (2018SJ-YZ03); Natural Science Foundation of Guangdong (2016A030313148); and China Agriculture Research System (CARS-46-03).

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Authors and Affiliations

  1. Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No 1 Xingyu Road, Liwan District, Guangzhou, 510380, Guangdong Province, China

    Jiajia Fan, Junjie Bai & Dongmei Ma

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  1. Jiajia Fan
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  2. Junjie Bai
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  3. Dongmei Ma
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Correspondence to Jiajia Fan.

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Fan, J., Bai, J. & Ma, D. Isolation and characterization of 40 SNP in largemouth bass (Micropterus salmoides). Conservation Genet Resour 12, 57–60 (2020). https://doi.org/10.1007/s12686-018-1076-2

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  • DOI: https://doi.org/10.1007/s12686-018-1076-2

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