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High 2,3-butanediol production from glycerol by Raoultella terrigena CECT 4519

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Abstract

Bioconversion of biodiesel-derived glycerol into 2,3-butanediol has received recently much attention due to its increasing surplus and its multiple uses in industry as bulk chemical. The influence of initial glycerol concentration on 2,3-butanediol production in batch runs has been studied. A concentration higher than 140 g/L produces an inhibitory effect on the final 2,3-butanediol concentration and its production rate. In batch mode, the highest yield respect to the theoretical maximum yield (71%) was reached employing 140 g/L as initial concentration 140 g/L. Based on these results, a high 2,3-butanediol production has been achieved through a fed-batch strategy. The reached 2,3-butanediol concentration was 90.5 g/L from pure glycerol and 80.5 g/L from raw glycerol. The 2,3-butanediol yield respect to the theoretical maximum yield was also improved through the fed-batch operation (90%). To date, this concentration is the highest produced amount employing as biocatalyst a non-pathogenic bacterium (level 1).

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Abbreviations

AA:

Acetic acid concentration (g/L)

Ac:

Acetoin concentration (g/L)

CDM:

Cell dry mass concentration (g/L)

Et:

Ethanol concentration (g/L)

G :

Glycerol concentration (g/L)

G C :

Consumed glycerol concentration (g/L)

G T :

Total fed glycerol concentration (g/L)

G 0 :

Initial glycerol concentration (g/L)

LA:

Lactic acid concentration (g/L)

P :

2,3-Butanediol productivity (g/L·h)

t :

Time (h)

Y :

2,3-Butanediol yield respect to the maximal theoretic yield (%)

2,3-BD:

2,3-Butanediol concentration (g/L)

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Acknowledgements

This work was supported by the Ministerio de Economía, Industria y Competitividad -MINECO-, Gobierno de España, under contract EUI2008-03600 and CTQ2010-11765-E.

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Authors and Affiliations

  1. Department of Chemical and Materials Engineering, Faculty of Chemistry, Universidad Complutense de Madrid, 28040, Madrid, Spain

    Miguel Ladero & Victoria E. Santos

  2. Facultad de Ciencias Experimentales, Universidad Francisco de Vitoria (UFV), Ctra. Pozuelo-Majadahonda km 1.800, 28223, Pozuelo de Alarcón, Madrid, Spain

    Vanessa Ripoll

  3. Biological Research Centre (CIB), Spanish National Research Council (CSIC), 28040, Madrid, Spain

    Alberto Rodríguez

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  1. Vanessa Ripoll
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Correspondence to Alberto Rodríguez or Victoria E. Santos.

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Ripoll, V., Rodríguez, A., Ladero, M. et al. High 2,3-butanediol production from glycerol by Raoultella terrigena CECT 4519. Bioprocess Biosyst Eng 43, 685–692 (2020). https://doi.org/10.1007/s00449-019-02266-8

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