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Hydra Attenuata, a Model System for Determining the Acute Lethal and Chronic Toxicity of Drinking and Natural Waters and Aqueous Solutions of Chemicals

  • Biological Methods of Water Treatment
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Abstract

Environmental assessment using living test organisms contribute to the timely detection of negative for human health pollutants. Hydra is a small freshwater organism that has been widely used in different types of bioassays to study the toxic potential of numerous chemical substances and is recently growing in popularity for assessing the impacts of water pollutants. Under normal conditions, adult hydra has a radially symmetric body of about 5 mm long with 6–8 straightened tentacles. Hydra is an appropriate bioindicator species because of their simple anatomy, clear morphological reactions in response to alterations in the aquatic environment, high reproduction rate and simplicity of laboratory-scale cultivation. Moreover, in the optimal cultivation environment, hydra reproduces by budding that enables in a short time obtaining a large number of genetically and morphologically identical animals for a bioassay. Here we propose a rapid bioassay for the assessment of the quality of aquatic environment including drinking water, water from natural environments sources and aqueous solutions of chemicals. This bioassay is based on the registration of survival and morphological reactions in hydra (Hydra attenuata), and allows us to determine the acute and chronic toxic effect of water pollutants. We also describe the optimal laboratory-scale cultivation conditions of hydra. Hydra attenuata bioassay of an aqueous medium can be recommended as a State Standard of Ukraine for assessing the quality of natural and drinking waters.

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References

  1. Standard DSTU 3959-2000. Okhorona dovkillia ta ratsionalne povodzhennia z resursami. Metodyki biotestuvannia vody. Nastanovy (Standard DSTU 3959-2000. Environmental Protection and Rational Resource Management. Water Biotesting Methods. Directives).

  2. Standard DSTU 4107-2002. Yakist vody. Vidbyrannia prob. 16. Nastanovy shchodo biotestuvannia prob (ISO 566716:1998, MOD) (Standard DSTU 4107-2002. Water Quality. Sampling. 16. Instructions on Biotesting of Samples (ISO 5667-16:1998, MOD).

  3. Stepan’yants, S.D., Kuznetsova, V.G., and Anokhin, B.A., Gidra: ot Abraama Tramble do nashykh dnei (Hydra: From Abraham Trembley to the Present Day), Moscow, St. Petersburg: Tov. nauch. izdanii KMK, 2003.

    Google Scholar 

  4. Goncharuk, V.V., Arkhipchuk, V.V., Terletska, G.V., and Korchak, G.I., Kompleksna otsinka yakosti fasovanykh vod, Visn. NAN Ukrainy, 2005, no. 3, pp. 47–58.

  5. Arkhipchuk, V.V., Blaise, Ch., and Malinovskaya, M.V., Use of Hydra for Chronic Toxicity Assessment of Waters Intended for Human Consumption, Environ. Pollut., 2006 vol. 142, pp. 200–211.

    Article  CAS  Google Scholar 

  6. Arkhipchuk, V.V., Malinovskaya, M.V., and Garanko, N.N., Cytogenetic study of organic and inorganic toxic substances on Allium cepa, Lactuca sativa and Hydra attenuata cells, Environ. Toxicol., 2000, vol. 15, pp. 338–344.

    Article  CAS  Google Scholar 

  7. Trottier, S., Blaise, C., Kusui, T., and Johnson, E.M., Acute toxicity assessment of aqueous samples using a microplate-based Hydra attenuata assay, Environ. Toxicol. Water Qual., 1997, vol. 12, pp. 265–271.

    Article  CAS  Google Scholar 

  8. Arkhipchuk, V.V., Malinovska, M.V., and Goncharuk, V.V., Patent of Ukraine 74934, Sposib vyznachennia khronichnoi toksychnosti vodnykh.

  9. Vanyushin, I.I., Eta udivitel’naya Artemiya, Akvarium, 1996, no. 4, pp. 26.

  10. Arkhipchuk, V.V. and Malinovskaya, M.V., Quality of water types in Ukraine evaluated by water tox bioassays, Environ. Toxicol., 2002, vol. 17, pp. 250–257.

    Article  CAS  Google Scholar 

  11. Johnson, E.M. and Gabel, B.E.G., Application of the Hydra assay for rapid detection of developmental hazards, J. Amer. Coil. Toxicol., 1982, no. 1, pp. 57–71.

    Article  Google Scholar 

  12. Blaise, C. and Kusui, T., Acute toxicity assessment of industrial effluents with a microplate-based Hydra attenuata assay, Environ. Toxicol. Water Qual., 1997, no. 12, pp. 5–14.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Dumanskii Institute of Colloid and Water Chemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    A. V. Nanieva, A. V. Pelishenko, V. F. Kovalenko & V. V. Goncharuk

Authors
  1. A. V. Nanieva
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  2. A. V. Pelishenko
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  3. V. F. Kovalenko
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  4. V. V. Goncharuk
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Corresponding author

Correspondence to A. V. Nanieva.

Additional information

Russian Text © The Author(s), 2019, published in Khimiya i Tekhnologiya Vody, 2019, Vol. 41, No. 5, pp. 555–562.

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Nanieva, A.V., Pelishenko, A.V., Kovalenko, V.F. et al. Hydra Attenuata, a Model System for Determining the Acute Lethal and Chronic Toxicity of Drinking and Natural Waters and Aqueous Solutions of Chemicals. J. Water Chem. Technol. 41, 329–333 (2019). https://doi.org/10.3103/S1063455X19050102

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  • DOI: https://doi.org/10.3103/S1063455X19050102

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