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Glycan-binding profile of DC-like cells

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Abstract

Modification of vaccine carriers by decoration with glycans can enhance binding to and even targeting of dendritic cells (DCs), thus augmenting vaccine efficacy. To find a specific glycan-“vector” it is necessary to know glycan-binding profile of DCs. This task is not trivial; the small number of circulating blood DCs available for isolation hinders screening and therefore advancement of the profiling. It would be more convenient to employ long-term cell cultures or even primary DCs from murine blood. We therefore examined whether THP-1 (human monocyte cell line) and DC2.4 (immature murine DC-like cell line) could serve as a model for human DCs. These cells were probed with a set of glycans previously identified as binding to circulating human CD14low/-CD16+CD83+ DCs. In addition, we tested a subpopulation of murine CD14low/-CD80+СD11c+CD16+ cells reported as relating to the human CD14low/-CD16+CD83+ cells. Manα1–3(Manα1–6)Manβ1–4GlcNAcβ1–4GlcNAcβ bound to both the cell lines and the murine CD14low/-CD80+СD11c+CD16+ cells. Primary cells, but not the cell cultures, were capable of binding GalNAcα1–3Galβ (Adi), the most potent ligand for binding to human circulating DCs. In conclusion, not one of the studied cell lines proved an adequate model for DCs processes involving lectin binding. Although the glycan-binding profile of BYRB-Rb (8.17)1Iem mouse DCs could prove useful for assessing human DCs, important glycan interactions were missing, a situation which was aggravated when employing cells from the BALB/c strain. Accordingly, one must treat results from murine work with caution when seeking vaccine targeting of human DCs, and certainly should avoid cell lines such as THP-1 and DC2.4 cells.

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Abbreviations

BSA:

Bovine serum albumin

DCs:

Dendritic cells

Glyc:

Glycan residue

fluo:

Fluorescein residue

LPS:

Lipopolysaccharide

PAA:

Polyacrylamide

PBS:

Phosphate buffer saline

PMA:

Phorbol 12-myristate 13-acetate

TBSCa:

tris-buffer, containing 2 mM CaCl2

TBSACa:

TBSCa, containing 2 mM BSA

Versene solution:

PBS containing 0.02% (w/v) EDTA

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Acknowledgments

This work was generously supported in part by grant 16-04-01084 of the Russian Foundation for Basic Research (E.R., E. M., D.A., S. Kh., G.P., S.Ts.), and EU FP7 Project UniVax (HEALTH-F3-2013-60173), grant number 601738 (N.B., K.M., E.R.).

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 16/10, Miklukho-Maklaya str., Moscow, 117997, Russia

    Eugenia M. Rapoport, Ekaterina V. Moiseeva, Dmitry A. Aronov, Sergey V. Khaidukov, Galina V. Pazynina, Svetlana V. Tsygankova, Ivan M. Ryzhov, Ivan M. Belyanchikov, Tatiana V. Tyrtysh & Nicolai V. Bovin

  2. Institute of Virology and Immunology, Sensemattstrasse 293, CH-3147, Mittelhäusern, Switzerland

    Kenneth C. McCullough

  3. Auckland University of Technology, Auckland, New Zealand

    Nicolai V. Bovin

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  1. Eugenia M. Rapoport
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  2. Ekaterina V. Moiseeva
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  3. Dmitry A. Aronov
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Correspondence to Nicolai V. Bovin.

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Thesaurus

•Classical dendritic cells – the DCs that are considered to be the most potent antigen-presenting cells, and therefore also referred to as “professional antigen-presenting cells”; also termed conventional DCs.

•Monocytes (Mo) – the cell population of myeloid origin characterized in humans as CD16neg with a CD14 expression relative to other cells described as CD14high.

•CD14low/-CD16+ CD83+ cells – the human blood mononuclear cells containing DC subpopulations

Electronic supplementary material

Fig. 1S

Probing of CD16+ and CD11c+ cells for Adi binding, assessed by flow cytometry. Flow cytometer plots show expression of CD11c+ and CD16+ on subpopulations of murine monocytes in terms of their SSC (A, B, E, F). Histograms show binding profiles of CD11c+ and CD16+ subpopulations to the glycoprobe Adi (C, D, G, H). The number given for the rectangle gate (A, B, E, F), and for the black histogram gate (C, D, G, H) represent the percentage of the positive CD16+ and CD11c+ cells within monocytes from blood of BALB/c and BYRB-Rb (8.17) strains. SSC, side scatter; FL, mean of fluorescence intensity. In the C, D, G, H histograms the logs of fluorescence intensity (x-axis) are plotted against cell number (y-axis, counts) (DOCX 143 kb)

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Rapoport, E.M., Moiseeva, E.V., Aronov, D.A. et al. Glycan-binding profile of DC-like cells. Glycoconj J 37, 129–138 (2020). https://doi.org/10.1007/s10719-019-09897-9

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