Skip to main content
SpringerLink
Log in

The Effects of α1-Adrenoreceptors’ Activation in the Interatrial Septum of Newborn and Adult Rats

  • RESEARCH ARTICLE
  • Published:
Moscow University Biological Sciences Bulletin Aims and scope Submit manuscript

Abstract—

Bioelectrical properties of various developing fragments of the heart undergo significant changes during pre- and postnatal ontogeny. However, the general pattern of the heart’s structure and the bioelectrical features of various parts of the “mature” heart are specifically determined in the early stages of embryogenesis. Specifically, the presence of myocardium with a phenotype, which is similar to a pacemaker, was revealed in the interatrial septum (IAS) of the mammalian heart using histological methods. However, the electrical activity in this structure remains understudied. The aim of the present study was to examine the capability of the IAS to generate action potentials spontaneously and the effects of adrenergic stimulation on the bioelectrical activity of the IAS. For this purpose, we recorded the resting and action potentials in the multicellular isolated perfused preparations of the IAS and left atrium from the rat heart at the end of day 1 and at day 60 of postnatal life using the standard microelectrode technique. We found that α1-adrenomimetic phenylephrine (PE) modified the patterns of action potentials in the samples of IAS and the atrial myocardium from the animals of both ages. In the resting samples without any electrical stimulation, PE induced the generation of action potential in the IAS but not in the atrium because of oscillations in the level of cytoplasmic calcium. Application of the inhibitor of hyperpolarization activated current (If) ZD 7288 led to a decrease in the rate of slow diastolic depolarization of action potential in the IAS pacemaker cells, a decrease in the frequency, and the appearance of train activity. Thus, in newborn and adult rats, the IAS myocardium generates spontaneous electrical activity. A necessary condition for the occurrence of pacemaker activity is adrenergic stimulation. The capability to generate spontaneous activity is probably associated with the presence of pacemaker current If in the IAS cardiomyocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

REFERENCES

  1. Moorman, A.F.M. and Christoffels, V.M., Cardiac chamber formation: Development, genes, and evolution, Int. J. Cardiol., 2003, vol. 83, no. 4, pp. 1223–1267.

    CAS  Google Scholar 

  2. Yanni, J., Tellez, J.O., Sutyagin, P.V., Boyett, M.R., and Dobrzynski, H.J., Structural remodelling of the sinoatrial node in obese old rats, J. Mol. Cell. Cardiol., 2010, vol. 48, no. 4, pp. 653–662.

    Article  CAS  Google Scholar 

  3. Kelly, R.G. and Buckingham, M.E., The anterior heart-forming field: voyage to the arterial pole of the heart, Trends Genet., 2002, vol. 18, no. 4, pp. 210–216.

    Article  CAS  Google Scholar 

  4. Davis, J.S., Hassanzadeh, S., Winitsky, S., Lin, H., Satorius, C., Vemuri, R., Aletras, A.H., Wen, H., and Epstein, N.D., The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation, Cell, 2001, vol. 107, no. 5, pp. 631–641.

    Article  CAS  Google Scholar 

  5. Rentschler, S., Vaidya, D.M., Tamaddon, H., Degenhardt, K., Sassoon, D., Morley, G.E., Jalife, J., and Fishman, G.I., Visualization and functional characterization of the developing murine cardiac conduction system, Development, 2001, vol. 128, no. 10, pp. 1785–1792.

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Højgaard, M.V., Holstein-Rathlou, N.H., Agner, E., and Kanters, J.K., Reproducibility of heart rate variability, blood pressure variability and baroreceptor sensitivity during rest and head-up tilt, Blood Press. Monit., 2005, vol. 10, no. 1, pp. 19–24.

    Article  Google Scholar 

  7. Moorman, A.F.M., Jong, F.De., Denyn, M.M., and Lamers, W.H., Development of the cardiac conduction system, Circ. Res., 1998, vol. 82, no. 6, pp. 629–644.

    Article  CAS  Google Scholar 

  8. Moorman, A.F.M. and Anderson, R.H., Development of the pulmonary vein, Int. J. Cardiol., 2011, vol. 147, no. 1, p. 182.

    Article  Google Scholar 

  9. Pustovit, K.B., Kuzmin, V.S., and Abramochkin, D.V., Diadenosine tetra- and pentaphosphates affect contractility and bioelectrical activity in the rat heart via P2 purinergic receptors, Naunyn Schmiedebergs Arch. Pharmacol., 2016, vol. 389, no. 3, pp. 303–313.

    Article  CAS  Google Scholar 

  10. Pustovit, K.B., Potekhina, V.M., Ivanova, A.D., Petrov, A.M., Abramochkin, D.V., and Kuzmin, V.S., Extracellular ATP and β-NAD alter electrical properties and cholinergic effects in the rat heart in age-specific manner, Pur.Sign., 2019, vol. 15, no. 1, pp. 107–111.

    CAS  Google Scholar 

  11. Hoffman, B.F. and Cranefield, P.F., Electrophysiology of the Heart, New York: McGraw-Hill, 1960.

    Google Scholar 

  12. O'Connell, T.D.O., Jensen, B.C., Baker, A.J., and Simpson, P.C., Cardiac alpha 1-adrenergic receptors: Novel aspects of expression, signaling mechanisms, physiologic function, and clinical importance, Pharmacol. Rev., 2014, vol. 66, no. 1, pp. 308–333.

    Article  Google Scholar 

  13. Egorov, Y.V., Kuzmin, V.S., Glukhov, A.V., and Rosenshtraukh, L.V., Electrophysiological characteristics, rhythm, disturbances and conduction discontinuities under autonomic stimulation in the rat pulmonary vein myocardium, J. Cardiovasc. Electrophysiol., 2015, vol. 26, no. 10, pp. 1130–1139.

    Article  Google Scholar 

  14. Xiao, R.-P., Zhu, W., Zheng, M., Cao, C., Zhang, Y., Lakatta, E.G., and Han, Q., Subtype-specific α1- and β-adrenoceptor signaling in the heart, Trends Pharmacol. Sci., 2006, vol. 27, no. 6, pp. 330–337.

    Article  CAS  Google Scholar 

  15. Reimann, F. and Ashcroft, F.M., Inwardly rectifying potassium channels, Curr. Opin. Cell Biol., 1999, vol. 11, no. 4, pp. 503–508.

    Article  CAS  Google Scholar 

  16. Anumonwo, J.M.B. and Lopatin, A.N., Cardiac strong inward rectifier potassium channels, J. Mol. Cell. Cardiol., 2010, vol. 48, no. 1, pp. 45–54.

    Article  CAS  Google Scholar 

  17. Oliva, C., Cohen, I.S., and Pennefather, P., The mechanism of rectification of iK1 in canine Purkinje myocytes, J. Gen. Physiol., 1990, vol. 96, no. 2, pp. 299–318.

    Article  CAS  Google Scholar 

  18. Ibarra, J., Morley, G.E., and Delmar, M., Dynamics of the inward rectifier K+ current during the action potential of guinea pig ventricular myocytes, Biophys. J., 1991, vol. 60, no. 6, pp. 1534–1539.

    Article  CAS  Google Scholar 

  19. Zhong, M., Rees, C.M., Terentyev, D., Choi, B., Koren, G., and Karma, A., NCX-mediated subcellular ca2d dynamics underlying early afterdepolarizations in lqt2cardiomyocytes, J. Biophys., 2018, vol. 115, no. 6, pp. 1019–1032.

    Article  CAS  Google Scholar 

  20. Kapoor, N., Tran, A., Kang, J., Zhang, R., Philipson, K.D., and Goldhaber, J., Regulation of calcium clock-mediated pacemaking by inositol-1,4,5-trisphosphate receptors in mouse sinoatrial nodal cells, J. Physiol., 2015, vol. 593, no. 12, pp. 2649–2663.

    Article  CAS  Google Scholar 

  21. Giladi, M., Tal, I., and Khananshvili, D., Structural features of ion transport and allosteric regulation in sodium-calcium exchanger (NCX) proteins, Front. Physiol., 2016, vol. 7, p. 30.

    Article  Google Scholar 

  22. Roell, W., Lewalter, T., Sasse, P., Tallini, Y.N., Choi, B.R., Breitbach, M., Doran, R., Becher, U.M., Hwang, S.M., Bostani, T., and Von Maltzahn, J., Engraftment  of connexin 43-expressing cells prevents post-infarct arrhythmia, Nature, 2007, vol. 450, no. 7171, pp. 819–824.

    Article  CAS  Google Scholar 

Download references

Funding

This study was supported by the Russian Foundation for Basic Research, project no. 18–34–00696.

Author information

Authors and Affiliations

  1. Department of Biology, Moscow State University, 119234, Moscow, Russia

    K. B. Pustovit

  2. Pirogov Russian National Research Medical University, 117997, Moscow, Russia

    K. B. Pustovit

  3. Kol’tsov Institute of Developmental Biology, 119334, Moscow, Russia

    E. A. Malolina

Authors
  1. K. B. Pustovit
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. A. Malolina
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. B. Pustovit.

Ethics declarations

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Additional information

Translated by M. Stepanichev

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pustovit, K.B., Malolina, E.A. The Effects of α1-Adrenoreceptors’ Activation in the Interatrial Septum of Newborn and Adult Rats. Moscow Univ. Biol.Sci. Bull. 74, 170–175 (2019). https://doi.org/10.3103/S009639251903009X

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S009639251903009X

Keywords:

Search

Navigation