Abstract
Sclerotium rolfsii lectin (SRL) exerts apoptotic effect against various cancer cells and an antitumor activity on mice with colon and breast cancer xenografts. The current study aimed to explore its exquisite carbohydrate specificity on human peripheral blood mononuclear cells (PBMCs) and leukemic T-cells. SRL, showed strong binding (>98%) to resting/activated PBMCs, leukemic Molt-4 and Jurkat cell lines. The glycans mediated binding to these cells was effectively blocked by mucin and fetuin, exhibiting 97% and 94% inhibition respectively. SRL showed mitogenic stimulation of PBMCs at 10 μg/ml as determined by thymidine incorporation assay. In contrast, lectin induced a dose dependent growth inhibition of Molt-4 cells with 58% inhibition at 25 μg/ml. Many common membrane receptors in activated PBMCs, Molt 4 and Jurkat cells were identified by lectin blotting. However, membrane receptors that are recognized by SRL in normal resting PBMCs were totally different and are high molecular weight glycoproteins. Treatment of membrane receptors with glycosidases prior to lectin probing, revealed that fucosylated Thomsen–Friedenreich(TF) antigen glycans are increasingly expressed on transformed Molt-4 leukemic cells compared to other cells. The findings highlight the opposite effects of SRL on transformed and normal hematopoietic cells by recognizing different glycan-receptors. SRL has promising potential for diagnostics and therapeutic applications in leukaemia.
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References
Couldrey, C., Green, J.E.: Metastases: the glycan connection. Breast Cancer Res. 2, 321–323 (2000)
Glavey, S.V., Huynh, D., Reagan, M.R., et al.: The cancer glycome: carbohydrates as mediators of metastasis. Blood Rev. 29, 269–279 (2015). https://doi.org/10.1016/j.blre.2015.01.003
Vajaria, B.N., Patel, P.S.: Glycosylation: a hallmark of cancer? Glycoconj. J. 34, 147–156 (2017)
Varki A, Kannagi R, Toole B, Stanley P. Glycosylation changes in cancer. In: Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, Darvill AG, Kinoshita T, Packer NH, Prestegard JH, Schnaar RL, Seeberger PH,editors. Essentials of Glycobiology [Internet]. 3rd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2015-2017. Chapter 47. 597-609 (2015)
Poiroux, G., Barre, A., van Damme, E.J.M., et al.: Plant lectins targeting o-glycans at the cell surface as tools for cancer diagnosis, prognosis and therapy. Int. J. Mol. Sci. 18, (2017). https://doi.org/10.3390/ijms18061232
Hashim, O.H., Jayapalan, J.J., Lee, C.-S.: Lectins: an effective tool for screening of potential cancer biomarkers. PeerJ. 5, e3784 (2017). https://doi.org/10.7717/peerj.3784
Valentiner, U., Fabian, S., Schumacher, U., Leathem, A.J.: The influence of dietary lectins on the cell proliferation of human breast cancer cell lines in vitro. Anticancer Res. 23, 1197–1206 (2003)
Ashraf, M.T., Khan, R.H.: Mitogenic lectins. Med. Sci. Monit. 9, RA265–RA269 (2003)
Shanmugham, L.N., Castellani, M.L., Salini, V., Falasca, K., Vecchiet, J., Conti, P., Petrarca, C.: Relevance of plant lectins in human cell biology and immunology. Riv. Biol. 99, 227–249 (2006)
Pujari, R., Nagre, N.N., Chachadi, V.B., et al.: Rhizoctonia bataticola lectin (RBL) induces mitogenesis and cytokine production in human PBMC via p38 MAPK and STAT-5 signaling pathways. Biochim. Biophys. Acta. 1800, 1268–1275 (2010). https://doi.org/10.1016/j.bbagen.2010.09.003
Nagre, N.N., Chachadi, V.B., Sundaram, P.M., Naik, R.S., Pujari, R., Shastry, P., Swamy, B.M., Inamdar, S.R.: A potent mitogenic lectin from the mycelia of a phytopathogenic fungus, Rhizoctonia bataticola, with complex sugar specificity and cytotoxic effect on human ovarian cancer cells. Glycoconj. J. 27, 375–386 (2010). https://doi.org/10.1007/s10719-010-9285-2
Inamdar, S.R., Savanur, M.A., Eligar, S.M., Chachadi, V.B., Nagre, N.N., Chen, C., Barclays, M., Ingle, A., Mahajan, P., Borges, A., Shastry, P., Kalraiya, R.D., Swamy, B.M., Rhodes, J.M., Yu, L.G.: The TF-antigen binding lectin from Sclerotium rolfsii inhibits growth of human colon cancer cells by inducing apoptosis in vitro and suppresses tumor growth in vivo. Glycobiology. 22, 1227–1235 (2012). https://doi.org/10.1093/glycob/cws090
Eligar, S.M., Pujari, R., Swamy, B.M., Shastry, P., Inamdar, S.R.: Sclerotium rolfsii lectin inhibits proliferation and induces apoptosis in human ovarian cancer cell line PA-1. Cell Prolif. 45, 397–403 (2012). https://doi.org/10.1111/j.1365-2184.2012.00831.x
Savanur, M.A., Eligar, S.M., Pujari, R., Chen, C., Mahajan, P., Borges, A., Shastry, P., Ingle, A., Kalraiya, R.D., Swamy, B.M., Rhodes, J.M., Yu, L.G., Inamdar, S.R.: Sclerotium rolfsii lectin induces stronger inhibition of proliferation in human breast cancer cells than normal human mammary epithelial cells by induction of cell apoptosis. PLoS One. 9, e110107 (2014). https://doi.org/10.1371/journal.pone.0110107
Singh, R.S., Walia, A.K.: Microbial lectins and their prospective mitogenic potential. Crit. Rev. Microbiol. 40, 329–347 (2014). https://doi.org/10.3109/1040841X.2012.733680
Porras, F., Lascurain, R., Chavez, R., et al.: Isolation of the receptor for Amaranthus leucocarpus lectin from murine naive thymocytes. Glycobiology. 10, 459–465 (2000)
Gorocica, P., Lascurain, R., Hemandez, P., et al.: Isolation of the receptor for Amaranthus leucocarpus lectin from murine peritoneal macrophages. Glycoconj. J. 15, 809–814 (1998)
Solorzano, C., Bouquelet, S., Pereyra, M.A., et al.: Isolation and characterization of the potential receptor for wheat germ agglutinin from human neutrophils. Glycoconj. J. 23, 591–598 (2006). https://doi.org/10.1007/s10719-006-8635-6
Swamy, B.M., Bhat, A.G., Hegde, G.V., et al.: Immunolocalization and functional role of Sclerotium rolfsii lectin in development of fungus by interaction with its endogenous receptor. Glycobiology. 14, 951–957 (2004). https://doi.org/10.1093/glycob/cwh130
Leonidas, D.D., Swamy, B.M., Hatzopoulos, G.N., Gonchigar, S.J., Chachadi, V.B., Inamdar, S.R., Zographos, S.E., Oikonomakos, N.G.: Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin. J. Mol. Biol. 368, 1145–1161 (2007). https://doi.org/10.1016/j.jmb.2007.02.092
Chachadi, V.B., Inamdar, S.R., Yu, L.-G., et al.: Exquisite binding specificity of Sclerotium rolfsii lectin toward TF-related O-linked mucin-type glycans. Glycoconj. J. 28, 49–56 (2011). https://doi.org/10.1007/s10719-011-9323-8
Anupama, S., Laha, P., Sharma, M., Pathak, K., Bane, S., Ingle, A.D., Gota, V., Kalraiya, R.D., Yu, L.G., Rhodes, J.M., Swamy, B.M., Inamdar, S.R.: Pharmacokinetics, biodistribution and antitumour effects of Sclerotium rolfsii lectin in mice. Oncol. Rep. 37, 2803–2810 (2017). https://doi.org/10.3892/or.2017.5545
Goldman, P., Carter, J.H., Wheeler, L.A.: Mutagenesis within the gastrointestinal tract determined by histidine auxotrophs of Salmonella typhimurium. Cancer. 45, 1068–1072 (1980). https://doi.org/10.1002/1097-0142(19800315)45:5+<1068::aid-cncr2820451307>3.0.co;2-l
Duk, M., Lisowska, E., Wu, J.H., Wu, A.M.: The biotin/avidin-mediated microtiter plate lectin assay with the use of chemically modified glycoprotein ligand. Anal. Biochem. 221, 266–272 (1994). https://doi.org/10.1006/abio.1994.1410
Pujari, R., Eligar, S.M., Kumar, N., et al.: Rhizoctonia bataticola lectin (RBL) induces caspase-8-mediated apoptosis in human T-cell leukemia cell lines but not in normal CD3 and CD34 positive cells. PLoS One. 8, e79311 (2013). https://doi.org/10.1371/journal.pone.0079311
Wessel, D., Flugge, U.I.: A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal. Biochem. 138, 141–143 (1984)
Wu, A.M., Wu, J.H., Tsai, M.S., et al.: Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsii. Life Sci. 69, 2039–2050 (2001)
Yu, L.-G.: The oncofetal Thomsen-Friedenreich carbohydrate antigen in cancer progression. Glycoconj. J. 24, 411–420 (2007). https://doi.org/10.1007/s10719-007-9034-3
Sindrewicz, P., Lian, L.-Y., Yu, L.-G.: Interaction of the oncofetal Thomsen-Friedenreich antigen with galectins in cancer progression and metastasis. Front. Oncol. 6, 79 (2016). https://doi.org/10.3389/fonc.2016.00079
Kolbl, A.C., Jeschke, U., Friese, K., Andergassen, U.: The role of TF- and Tn-antigens in breast cancer metastasis. Histol. Histopathol. 31, 613–621 (2016). https://doi.org/10.14670/HH-11-722
Li, F., Glinskii, O.V., Mooney, B.P., et al.: Cell surface Thomsen-Friedenreich proteome profiling of metastatic prostate cancer cells reveals potential link with cancer stem cell-like phenotype. Oncotarget. 8, 98598–98608 (2017). https://doi.org/10.18632/oncotarget.21985
Cao, Y., Karsten, U., Zerban, H., Bannasch, P.: Expression of MUC1, Thomsen-Friedenreich-related antigens, and cytokeratin 19 in human renal cell carcinomas and tubular clear cell lesions. Virchows Arch. 436, 119–126 (2000)
Yi, B., Zhang, M., Schwartz-Albiez, R., Cao, Y.: Mechanisms of the apoptosis induced by CD176 antibody in human leukemic cells. Int. J. Oncol. 38, 1565–1573 (2011). https://doi.org/10.3892/ijo.2011.992
Yi, B., Zhang, Z., Zhang, M., Schwartz-Albiez, R., Cao, Y.: CD176 antiserum treatment leads to a therapeutic response in a murine model of leukemia. Oncol. Rep. 30, 1841–1847 (2013). https://doi.org/10.3892/or.2013.2639
Wu, A.M., Wu, J.H., Yang, Z., et al.: Differential contributions of recognition factors of two plant lectins -Amaranthus caudatus lectin and Arachis hypogea agglutinin, reacting with Thomsen-Friedenreich disaccharide (Galbeta1-3GalNAcalpha1-Ser/Thr). Biochimie. 90, 1769–1780 (2008). https://doi.org/10.1016/j.biochi.2008.08.001
Cooper, H.S.: Reuter VE Peanut lectin-binding sites in polyps of the colon and rectum. Adenomas, hyperplastic polyps, and adenomas with in situ carcinoma. Lab. Investig. 49, 655–661 (1983)
Orntoft, T.F., Mors, N.P., Eriksen, G., Jacobsen, N.O., Poulsen, H.S.: Comparative immunoperoxidase demonstration of T-antigens in human colorectal carcinomas and morphologically abnormal mucosa. Cancer Res. 45, 447–452 (1985)
Sumar, N., Bodman, K.B., Rademacher, T.W., et al.: Analysis of glycosylation changes in IgG using lectins. J. Immunol. Methods. 131, 127–136 (1990)
Yu, L.G., Fernig, D.G., White, M.R., et al.: Edible mushroom (Agaricus bisporus) lectin, which reversibly inhibits epithelial cell proliferation, blocks nuclear localization sequence-dependent nuclear protein import. J. Biol. Chem. 274, 4890–4899 (1999)
Itzkowitz, S.H., Yuan, M., Montgomery, C.K., Kjeldsen, T., Takahashi, H.K., Bigbee, W.L., Kim, Y.S.: Expression of Tn, sialosyl-Tn, and T antigens in human colon cancer. Cancer Res. 49, 197–204 (1989)
Stein, R., Chen, S., Grossman, W., Goldenberg, D.M.: Human lung carcinoma monoclonal antibody specific for the Thomsen-Friedenreich antigen. Cancer Res. 49, 32–37 (1989)
Baldus, S.E., Zirbes, T.K., Glossmann, J., Fromm, S., Hanisch, F.G., Mönig, S.P., Schröder, W., Schneider, P.M., Flucke, U., Karsten, U., Thiele, J., Hölscher, A.H., Dienes, H.P.: Immunoreactivity of monoclonal antibody BW835 represents a marker of progression and prognosis in early gastric cancer. Oncology. 61, 147–155 (2001). https://doi.org/10.1159/000055366
Baldus, S.E., Zirbes, T.K., Hanisch, F.G., Kunze, D., Shafizadeh, S.T., Nolden, S., Mönig, S.P., Schneider, P.M., Karsten, U., Thiele, J., Hölscher, A.H., Dienes, H.P.: Thomsen-Friedenreich antigen presents as a prognostic factor in colorectal carcinoma: a clinicopathologic study of 264 patients. Cancer. 88, 1536–1543 (2000)
Kurtenkov, O., Klaamas, K., Rittenhouse-Olson, K., Vahter, L., Sergejev, B., Miljukhina, L., Shljapnikova, L.: IgG immune response to tumor-associated carbohydrate antigens (TF, Tn, alphaGal) in patients with breast cancer: impact of neoadjuvant chemotherapy and relation to the survival. Exp. Oncol. 27, 136–140 (2005)
Kawagishi, H., Nomura, A., Mizuno, T., Kimura, A., Chiba, S.: Isolation and characterization of a lectin from Grifola frondosa fruiting bodies. Biochim. Biophys. Acta. 1034, 247–252 (1990)
Yu, L., Fernig, D.G., Smith, J.A., Milton, J.D., Rhodes, J.M.: Reversible inhibition of proliferation of epithelial cell lines by Agaricus bisporus (edible mushroom) lectin. Cancer Res. 53, 4627–4632 (1993)
Mahajan, R.G., Patil, S.I., Mohan, D.R.K., Shastry, P.: Pleurotus Eous mushroom lectin (PEL) with mixed carbohydrate inhibition and antiproliferative activity on tumor cell lines. J. Biochem. Mol. Biol. Biophys. 6, 341–345 (2002). https://doi.org/10.1080/1025814021000008558
Wang, H.X., Ng, T.B., Liu, W.K., et al.: Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicum. Int. J. Pept. Protein Res. 46, 508–513 (1995)
Wang, H., Gao, J., Ng, T.B.: A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem. Biophys. Res. Commun. 275, 810–816 (2000). https://doi.org/10.1006/bbrc.2000.3373
Wang, H., Ng, T.B., Liu, Q.: A novel lectin from the wild mushroom Polyporus adusta. Biochem. Biophys. Res. Commun. 307, 535–539 (2003)
Ngai, P.H.K., Ng, T.B.: A mushroom (Ganoderma capense) lectin with spectacular thermostability, potent mitogenic activity on splenocytes, and antiproliferative activity toward tumor cells. Biochem. Biophys. Res. Commun. 314, 988–993 (2004)
Singh Bains, J., Singh, J., Kamboj, S.S., Nijjar, K.K., Agrewala, J.N., Kumar, V., Kumar, A., Saxena, A.K.: Mitogenic and anti-proliferative activity of a lectin from the tubers of Voodoo lily (Sauromatum venosum). Biochim. Biophys. Acta. 1723, 163–174 (2005). https://doi.org/10.1016/j.bbagen.2005.02.006
Nicolson, G.L.: Trans-membrane control of the receptors on normal and tumor cells. II. Surface changes associated with transformation and malignancy. Biochim. Biophys. Acta. 458, 1–72 (1976)
Speckart, S.F., Boldt, D.H., MacDermott, R.P.: Chronic lymphatic leukemia (CLL): cell surface changes detected by lectin binding and their relation to altered glycosyltransferase activity. Blood. 52, 681–695 (1978)
Endo, Y., Kobata, A.: Partial purification and characterization of an endo-alpha-N-acetylgalactosaminidase from the culture of medium of Diplococcus pneumoniae. J. Biochem. 80, 1–8 (1976)
Dube, D.H., Bertozzi, C.R.: Glycans in cancer and inflammation--potential for therapeutics and diagnostics. Nat. Rev. Drug Discov. 4, 477–488 (2005). https://doi.org/10.1038/nrd1751
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This work was supported by the UGC-Start up grant (F.30-117/2015 (BSR)), University Grant Commission, New Delhi.
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Chachadi, V.B., Pujari, R., Shastry, P. et al. Sclerotium rolfsii lectin induces opposite effects on normal PBMCs and leukemic Molt-4 cells by recognising TF antigen and its variants as receptors. Glycoconj J 37, 251–261 (2020). https://doi.org/10.1007/s10719-019-09905-y
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DOI: https://doi.org/10.1007/s10719-019-09905-y