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Physical mapping of an adult plant stripe rust resistance gene from Triticum monococcum

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Abstract

Stripe rust (Puccinia striiformis f. sp. tritici) is one of the major devastating disease which causes large reduction in wheat yield. T. monococcum is an attractive diploid species for gene discovery in wheat with smaller genome size of 5700 Mb compared to 17,300 Mb of bread wheat. An adult plant stripe rust resistance QTL QYrtm.pau-2A was mapped on chromosome 2A flanked by two SSR markers Xwmc170 and Xwmc407. In the present study, two gene based markers Pau_Ta2AL_Gene45 and Pau_Ta2AL_Gene54 developed from 2A specific ESTs were found to map close to QYrtmpau-2A to narrow down the region for candidate gene identification. Utilizing sequence information of these two markers, four BAC clones were identified from the Minimum Tiling Path of 2AL assembly and were sequenced. SSR markers were designed from these BAC sequences and mapped to chromosome 2A. A 50 Mb region of wheat chromomse 2A was identified to harbor stripe rust resistance gene of T. monococcum. Gene based markers identified in the present investigation can be used for marker assisted introgression of QYrtm.pau-2A from T. monococcum to cultivated wheat.

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Abbreviations

RIL:

Recombinant inbred lines

QTL:

Quantitative trait loci

AC:

Bacterial artificial chromosome

EST:

Expressed sequence tags

SSR:

Simple sequence repeats

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Acknowledgement

The financial support provided by DBT BT/IWGSC/03/TF/2008 under the Grant is gratefully acknowledged.

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Author notes

  1. Parampreet Kaur

    Present address: School of Organic Farming, Punjab Agricultural University, Ludhiana, India

  2. Kuldeep Singh

    Present address: ICAR-National Bureau of Plant Genetic Resources, New Delhi, India

Authors and Affiliations

  1. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India

    Priti Sharma, Preeni Bawa, Bharat Yadav, Parampreet Kaur, Suruchi Jindal, Inderjit Yadav, Satinder Kaur, Kuldeep Singh & Parveen Chhuneja

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  1. Priti Sharma
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  2. Preeni Bawa
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  3. Bharat Yadav
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  4. Parampreet Kaur
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  6. Inderjit Yadav
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Correspondence to Priti Sharma.

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Sharma, P., Bawa, P., Yadav, B. et al. Physical mapping of an adult plant stripe rust resistance gene from Triticum monococcum. J. Plant Biochem. Biotechnol. 29, 47–55 (2020). https://doi.org/10.1007/s13562-019-00511-5

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