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QTLs for cell membrane stability and flag leaf area under drought stress in a wheat RIL population

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Abstract

A population of 206 recombinant inbred lines (RILs F9–F10) derived from wheat cross WL711/C306 was phenotyped for morpho-physiological traits such as flag leaf area (FLA), flag leaf length (FLL), flag leaf width (FLW), and cell membrane stability (CMS) under water deficit stress (WDS) environment. High yielding cultivar, WL711 had higher FLA than the medium yielding cultivar C306 across trials under both environments. Parent cultivar C306 maintained membrane integrity while WL711 showed higher membrane damage under WDS. The RIL population showed considerable variation, normal distribution and transgressive segregation for FLA, FLL, FLW and CMS under WDS. The genetic linkage map of WL711/C306 RIL population was constructed comprising of 346 markers. The total map distance was 4526.8 cM with an averaged interval of 12.9 cM between adjacent markers. Major consistent QTL for FLA, FLL, FLW, and CMS were identified on chromosomes 2DS and 3BS respectively in the WL711/C306 RIL population under WDS. The major QTL for FLA, qFLAWD.2D.1 which expressed in multiple environments and for CMS, qCMSWD.3B.3 and qCMSWD.3B.4, accounted for a large proportion of phenotypic variance (PV) with positive allele being contributed by C306, a drought resistant (DR) parent. QTL qFLAWD.2D.1 for FLA co-located with QTL for grain number (GN) and days to flowering (DTF) while QTL qCMSWD.3B.3 and qCMS.3B.4 co-located with QTL for grain yield and its components, days to flowering, canopy temperature and coleoptiles length as reported in our previous publications on the WL711/C306 population (Shukla et al. in Euphytica 203:449–467, 2015; Singh et al. in J Plant Biochem Biotechnol 24:324–330, 2015). Two candidate genes Ghd7 for grain yield and heading date and OsCDK4 for calcium dependent protein kinases were identified in the 2DS and 3BS QTL regions respectively on comparison with gene content of rice chromosomes 7 and 1 respectively. Hence, QTLs qFLAWD.2D.1 and qCMSWD.3B.3 are potential target regions for fine mapping and marker assisted selection for FLA and CMS respectively in wheat under water deficit environments.

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Abbreviations

FLA:

Flag leaf area

CMS:

Cell membrane stability

QTL:

Quantitative trait loci

RIL:

Recombinant inbred lines

FLL:

Flag leaf length

FLW:

Flag leaf width

GY:

Grain yield

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Acknowledgements

We are thankful to the Indian Council of Agricultural Research for financial support to Dr. R. Khanna-Chopra and Dr. N. K. Singh under the “Network project on Transgenics in crops and functional genomics.

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Authors and Affiliations

  1. Stress Physiology Laboratory, Water Technology Centre, Indian Agricultural Research Institute (IARI), New Delhi, 110012, India

    Renu Khanna-Chopra, Kalpana Singh & Sanyukta Shukla

  2. National Research Center on Plant Biotechnology, IARI, New Delhi, 110012, India

    Suhas Kadam & Nagendra Kumar Singh

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  1. Renu Khanna-Chopra
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13562_2019_534_MOESM1_ESM.tif

Supplementary Fig. 1 Frequency distribution of flag leaf area (a), flag leaf length (b) and flag leaf width (c) in the parents and the WL711/C306 RIL population under irrigated and water deficit stress treatment in Delhi during the year 2008–2009 and 2009–2010 and Indore 2008–2009 trials. Histograms with the solid and open bars represent the frequencies of flag leaf area, flag leaf length and flag leaf width of RILs under irrigated and water deficit stress treatments respectively. W-WL711, C-C306 (TIFF 486 kb)

13562_2019_534_MOESM2_ESM.tif

Supplementary Fig. 2 Frequency distribution of Cell membrane stability (CMS, expressed as % relative injury) in the flag leaf at post-anthesis stage (A + 14) of the parents and the WL711/C306 RILs under water deficit stress conditions. W- WL711, C- 306 (TIFF 80 kb)

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Khanna-Chopra, R., Singh, K., Shukla, S. et al. QTLs for cell membrane stability and flag leaf area under drought stress in a wheat RIL population. J. Plant Biochem. Biotechnol. 29, 276–286 (2020). https://doi.org/10.1007/s13562-019-00534-y

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