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Potential of calcium nitrate to mitigate the aluminum toxicity in Phaseolus vulgaris: effects on morphoanatomical traits, mineral nutrition and photosynthesis

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Abstract

Common bean (Phaseolus vulgaris) cultivation occurs mainly in regions with acidic soils, where high aluminum (Al) concentration is a major constraint to crop production. In this study, we evaluated tolerance and sensitivity traits to Al exposure and calcium (Ca) deficiency in bean plants, and determined the efficiency of Ca to mitigate the toxic Al effects. Two bean cultivars (BRS Estilo and Campos Gerais) were grown in three soil conditions: (I) soil liming with calcium hydroxide Ca(OH)2 and Al unavailable (−Al+Ca); (II) fertilized soil with calcium nitrate [Ca(NO3)2·4H2O] and Al available (+Al+Ca); and (III) soil without Ca addition and Al available (+Al−Ca). At the beginning of the reproductive stage, we evaluated the photosynthetic processes, mineral nutrition, and leaf anatomy and morphological traits of plants. The photosynthetic parameters were good tools for monitor Al sensitivity in bean. +Al+Ca soil treatment mitigated the deleterious effects of Al on growth and mineral nutrition of both bean cultivars. However, Ca did not prevent the toxic effects of Al on leaf anatomy. Al stress and Ca deficiency caused negative effects on nutrient content, photosynthetic activity and leaf anatomy of bean plants. Calcium mitigated Al toxicity, primarily in the Campos Gerais cultivar, showing the potential to improve bean crop productivity in acid soils.

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Acknowledgements

The authors thank the Funding Authority for Studies and Projects (FINEP, grant no. 0519/11), the National Council for Scientific and Technological Development (CNPq, grants no. 551456/2010-8 and 552689/2011-4) and the Goiano Federal Institute of Education, Science and Technology, Rio Verde campus (IFGoiano-RV, grant no. DPPG 045/2014) for providing financial support. CVV, CM and AAS are grateful to the Coordination for the Improvement of Higher Education Personnel (CAPES) for fellowships.

Author contributions

CVV, AAC and GC designed the study. CVV, AMC and CM conducted the experiments and performed the physiological measurements. CVV, AMC and KPB and AAR performed the anatomical analysis. CVV performed nutritional analysis. All author discussed the data. AAS constructed the graphics. CVV, CM and AAS wrote the manuscript with contributions from all other authors. All authors read and approved the manuscript.

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Authors and Affiliations

  1. Laboratory of Ecophysiology and Plant Productivity, Goiano Federal Institute of Education Science and Technology, Campus Rio Verde, PO Box 66, Rio Verde, Goiás, 75901-970, Brazil

    Camila Vilela Vasconcelos, Alan Carlos Costa, Caroline Müller, Gustavo Castoldi, Andréia Mendes Costa, Kássia de Paula Barbosa & Adinan Alves da Silva

  2. Laboratory of Plant Anatomy, Goiano Federal Institute of Education Science and Technology, Campus Rio Verde, PO Box 66, Rio Verde, Goiás, 75901-970, Brazil

    Arthur Almeida Rodrigues

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  1. Camila Vilela Vasconcelos
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  2. Alan Carlos Costa
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  3. Caroline Müller
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  4. Gustavo Castoldi
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  5. Andréia Mendes Costa
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  6. Kássia de Paula Barbosa
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  7. Arthur Almeida Rodrigues
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  8. Adinan Alves da Silva
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Correspondence to Alan Carlos Costa.

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Vasconcelos, C.V., Costa, A.C., Müller, C. et al. Potential of calcium nitrate to mitigate the aluminum toxicity in Phaseolus vulgaris: effects on morphoanatomical traits, mineral nutrition and photosynthesis. Ecotoxicology 29, 203–216 (2020). https://doi.org/10.1007/s10646-020-02168-6

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