Abstract
This study aimed to evaluate the influence of germination on the content of protein, lipids, fiber, ashes, tryptophan, vitamin C, total phenolic and total flavonoid compounds, as well as on the protein digestibility and antioxidant activity of chia seeds germinated for 1, 2, 3, and 4 days. The results showed that germination for 2 days augmented the protein content of chia seeds by 13% while fiber, tryptophan, total phenolic and flavonoid contents increased by 46%, 93%, 300%, and 197%, respectively, after 4 days of germination. Vitamin C was not detected in dry seeds but increased up to 2.33 mg/100 g at fourth day of germination. The antioxidant capacity increased approximately 100% but protein digestibility decreased by 14% at day 4 of germination. Germination can be a good method to increase the nutritional and nutraceutical potential of chia seed for its use in the design of functional foods.
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References
Álvarez-Jubete L, Wijngaard H, Arendt EK, Gallagher E. Polyphenol composition and in vitro antioxidant activity of amaranth, quinoa, buckwheat and wheat as affected by sprouting and baking. Food Chem. 119: 770-778 (2010)
Anderson JW, Baird P, Davis RH, Ferreri S, Knudtson M, Koraym A, Waters V, William CL. Health benefits of dietary fiber. Nutr. Rev. 67: 188-205 (2009)
AOAC. Official Method of Analysis of AOAC Intl. 16th ed. Methods 925.09, 954.01, 920.39, 991.43, and 923.03. Association of Official Analytical Chemists, Washington, DC, USA (1995)
Ayerza R. Seed composition of two chia (Salvia hispanica L.) genotypes which differ in seed color. Emir. J. Food Agr. 25: 495-500 (2013)
Ayerza R, Coates W. Some quality components of four chia (Salvia hispanica L.) genotypes grown under tropical coastal desert ecosystem conditions. Asian J. Plant Sci. 8: 301-307 (2009)
Benítez V, Cantera S, Aguilera Y, Mollá E, Esteban RM, Díaz MF, Martín-Cabrejas MA. Impact of germination on starch, dietary fiber and physicochemical properties in non-conventional legumes. Food Res. Int. 50: 64-69 (2013)
Camacho L, Sierra C, Campos R, Guzman R, Marcus, D. Nutritional changes caused by the germination of legumes commonly eaten in Chile. Arch. Latinoam. Nutr. 42: 283-290 (1992)
Dikshit M, Ghadle M. Effect of sprouting on nutrients, antinutrients and in vitro digestibility of de MACS-13 soybean variety. Plant Foods Hum. Nutr. 58: 1-11 (2003)
Dudonné S, Vitrac J, Coutiere P, Woillez M, Mérillon J-M. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD and ORAC assays. J. Agric. Food Chem. 57: 1768-1774 (2009)
Dueñas M, Hernández T, Estrella I, Fernández D. Germination as a process to increase the polyphenol content and antioxidant activity of lupin seeds (Lupinus angustifolius L.). Food Chem. 117: 599-607 (2009)
Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR. Iron quelating activity, phenol and flavonoid content of some medicinal plants from Iran. Afr. J. Biotechnol. 7: 3188-3192 (2008)
Fernandez-Orozco R, Frias J, Zielinsky H, Piskula MK, Kozlowska H, Vidal-Valverde C. Kinetic study of the antioxidant compounds and antioxidant capacity during germination of Vigna radiata cv. emmerald, Glycine max cv. jutro, and Glycine max cv. merit. Food Chem. 111: 622-630 (2008)
Frias J, Miranda ML, Doblado R, Vidal-Valverde C. Effect of germination and fermentation on the antioxidant vitamin content and antioxidant capacity of Lupinus albus L. var. Multolupa. Food Chem. 92: 211-220 (2005)
Ghavidel RA, Prakash J. The impact of germination and dehulling on nutrients, antinutrients, in vitro iron and calcium bioavailability and in vitro starch and protein digestibility of some legume seeds. LWT-Food Sci. Technol. 40: 1292-1299 (2007)
Gómez-Favela MA, Gutiérrez-Dorado R, Cuevas-Rodríguez EO, Canizales-Román VA, León-Sicairos C, Milán-Carrillo J, Reyes-Moreno C. Improvement of chia seeds with antioxidant activity, GABA, essential amino acids, and dietary fiber by controlled germination bioprocess. Plant Foods Hum. Nutr. 72: 345-352 (2017)
Hsu HW, Vavak DL, Satterlee LD, Miller GA. A multienzyme technique for estimating protein digestibility. J. Food Sci. 42: 1269-1273 (1977)
Kim D-K, Jeong SC, Gorinstein S, Chon S-U. Total polyphenols, antioxidant and antiproliferative activities of different extracts in mungbean seeds and sprouts. Plant Foods Hum. Nutr. 67: 71-75 (2012)
Martín-Cabrejas M, Ariza N, Esteban R, Mollá E, Waldron K, López-Andreu F. Effect of germination on the carbohydrate composition of the dietary fiber of peas (Pisum sativum L.). J. Agr. Food Chem. 51: 1254-1259 (2003)
Martínez-Cruz O, Paredes-López O. Phytochemical profile and nutraceutical potential of chia seeds (Salvia hispanica L.) by ultra high performance liquid chromatography. J. Chromatogr. A. 1346: 43-48 (2014)
Martínez-Villaluenga C, Gulewicz P, Frías J, Gulewicz K, Vidal-Valverde C. Assesment of protein fractions of three cultivars of Pisum sativum L.: effect of germination. Eur. Food Res. Technol. 6: 1465-1478 (2008)
Ndakidemi PA, Dakora FD. Legume seed flavonoids and nitrogenous metabolites as signals and protectants in early seedling development. Funct. Plant Biol. 30: 729-745 (2003)
Olivos-Lugo BL, Valdivia-López MA, Tecante A. Thermal and physicochemical properties and nutritional value of the protein fraction of Mexican chia seed (Salvia hispanica L.). Food Sci. Technol. Int. 16: 89-96 (2010)
Pereira PE, Barros TS, da Silva SF, Walessa NN, Oliveira FR, de Sousa LM. Light regime and temperature on seed germination in Salvia hispanica L. Acta Sci. Agron. 38: 513-519 (2016)
Ranganna S. Handbook of Analysis and Quality Control for Fruit and Vegetable Products. Tata McGraw Hill Publishing Co. Ltd., New Delhi, India. pp. 190-210 (1986)
Reyes-Caudillo E, Tecante A, Valdivia-López M. Dietary fibre content and antioxidant activity of phenolic compounds present in Mexican chia (Salvia hispanica L.) seeds. Food Chem. 107: 656-663 (2008)
Rumiyati R, James AP, Jayasena V. Effect of germination on the nutritional and protein profile of Australian sweet lupin (Lupinus angustifolius L.). Food Nutr. Sci. 3: 621-626 (2012)
Sandoval-Oliveros MR, Paredes-López O. Isolation and characterization of proteins from chia seeds (Salvia hispanica L.). J. Agr. Food Chem. 61: 193-201 (2013)
Singleton V, Orthofer R, Lamuela R. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method. Enzymol. 299: 152-178 (1999)
Spies JR, Chambers DC. Chemical determination of tryptophan. Anal. Chem. 20: 30-39 (1948)
Taraseviciene Z, Danilcenko H, Ariene E, Paulauskiene A, Gajewski M. Changes in some chemical components during germination of broccoli seeds. Not. Bot. Hort. Agrobot. Cluj. 37: 173-176 (2009)
Ullah R, Nadeem M, Khalique A, Imran M, Mehmood S, Javid A, Hussain J. Nutritional and therapeutic perspectives of chia (Salvia hispanica L.): a review. J. Food Sci. Technol. 53: 1750-1758 (2016)
Urbano G, Aranda P, Vílchez A, Aranda C, Cabrera L, Porres JM, López-Jurado M. Effects of germination on the composition and nutritive value of proteins in Pisum sativum L. Food Chem. 93: 671-679 (2005)
USDA. Seeds, chia seeds, dried. In: National nutrient database for standard reference. Full report, NDB Number 12006. US Department of Agriculture, USA (2016)
Vázquez-Ovando JA, Rosado-Rubio JG, Chel-Guerrero LA, Betancur-Ancona DA. Dry processing of chia (Salvia hispanica L.) flour: chemical characterization of fiber and protein. CyTA-J. Food 8: 117-127 (2010)
Xu M-J, Dong J-F, Zhu M-Y. Effects of germination conditions on ascorbic acid level and yield of soybean sprouts. J. Sci. Food Agr. 85: 943-947 (2005)
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This research was financed by Instituto Politécnico Nacional through the Project SIP20150652.
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Beltrán-Orozco, M.C., Martínez-Olguín, A. & Robles-Ramírez, M.C. Changes in the nutritional composition and antioxidant capacity of chia seeds (Salvia hispanica L.) during germination process. Food Sci Biotechnol 29, 751–757 (2020). https://doi.org/10.1007/s10068-019-00726-1
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DOI: https://doi.org/10.1007/s10068-019-00726-1