Abstract
Squalene is a cancer chemo-preventive and skin protective agent with high commercial demand. Here, we report for the first time that the green tea (Camellia sinensis) leaves is a surprisingly rich plant-based source of squalene. Young and tender leaves and old and turf leaves were collected at four different collecting seasons (April–August). Lipophilic compounds in the leaves and commercial green teas were extracted with hexane. The squalene contents in the hexane extracts varied greatly with the types of the leaves and collecting seasons. The hexane extract of turf leaves contained significantly higher contents of squalene than the extract of tender leaves. The hexane extract of the turf leaves collected in August contained the highest content of squalene (29.2 g/kg extract). This represents the first report on the qualitative and quantitative information on squalene in green tea leaves.
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References
Beltrán G, Bucheli ME, Aguilera MP, Belaj A, Jimenez A. Squalene in virgin olive oil: screening of variability in olive cultivars. Eur. J. Lipid Sci. Technol. 118: 1250-1253 (2016)
Budge SM, Barry, C. Determination of squalene in edible oils by transmethylation and GC analysis. MethodsX 6: 15-21 (2019)
Choi SJ, Park SY, Park JS, Park S, Jung MY. Contents and compositions of policosanols in green tea (Camellia sinensis) leaves. Food Chem. 204: 94-101 (2016)
Frega N, Bocci F, Lercker G. Direct gas chromatographic analysis of the unsaponifiable fraction of different oils with a polar capillary column. J. Am. Oil Chem. Soc. 69: 447–450 (1992)
Grigoriadou D, Androulaki A, Psomiadou E, Tsimidou MZ. Solid phase extraction in the analysis of squalene and tocopherols in olive oil. Food Chem. 105: 675-680 (2007)
He HP, Corke H. Oil and squalene in Amaranthus grain and leaf. J. Agric. Food Chem. 51: 7913-7920 (2003)
He HP, Cai Y, Sun M, Corke, H. Extraction and purification of squalene from Amaranthus grain. J. Agric. Food Chem. 50: 368-372 (2002)
Huang Z-R, Lin Y-K, Fang J-Y. Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. Molecules 14: 540-554 (2009)
Jung DM, Lee MJ, Yoon SH, Jung MY. A gas chromatography tandem quadrupole mass spectrometric analysis of policosanols in commercial vegetable oils. J. Food Sci. 76: C891-C899 (2011)
Lanzon A, Albi T, Cert A, Gracián J. Hydrocarbon fraction of virgin olive oil and changes resulting from refining. J. Am. Oil Chem. Soc. 71: 285-291 (1994)
Nergiz C, Celikkale DC. The effect of consecutive steps of refining on squalene content of vegetable oils. J. Food Sci. Technol. 48: 382-385 (2011)
Newmarks HL. Olive oil, and cancer risk: review and hypothesis. Ann. N. Y. Acad. Sci. 889: 193-203 (2006)
Ortega JAA, Zavala AM, Hernández MC, Reyes JD. Analysis of trans fatty acids production and squalene variation during amaranth oil extraction. Cent. Eur. J. Chem. 10: 1773-1778 (2012)
Oya Y, Mondal A, Rawangkan A, Umsumarng S, Iida K, Watanabe T, Kanno M, Suzuki K, Li Z, Kagechika H, Shudo K, Fujiki H, Suganuma M. Down-regulation of histone deacetylase 4, -5 and -6 as a mechanism of synergistic enhancement of apoptosis in human lung cancer cells treated with the combination of a synthetic retinoid, Am80 and green tea catechin. J. Nutr. Biochem. 42: 7-16 (2017)
Pokkanta P, Sookwong S, Tanang M, Setchaiyan S, Boontakham P, Mahatheeranont S. Simultaneous determination of tocols, γ-oryzanols, phytosterols, squalene, cholecalciferol and phylloquinone in rice bran and vegetable oil samples. Food Chem. 271: 630-638 (2019)
Popa O, Băbeanu NL, Popa I, Niţă S, Dinu-Pârvu CL. Methods for obtaining and determination of squalene from natural sources. BioMed Res. Int. 2015: 1-16 (2015)
Reddy LH, Couvreur P. Squalene: A natural triterpene for use in disease management and therapy. Adv. Drug Deliv. Rev. 61: 1412-1426 (2009)
Rudzińska M, Górnaś P, Raczyk M, Soliven A. Sterols and squalene in apricot (Prunus armeniaca L.) kernel oils: the variety as a key factor. J. Nat. Prod. Res. 31: 84-88 (2017)
Salvo A, La Torre GL, Rotondo A, Mangano V, Casale KE, Pellizzeri V, Clodoveo ML, Corbo F, Cicero N, Dugo G. Determination of squalene in organic extra virgin olive oils (EVOOs) by UPLC/PDA using a single-step SPE sample preparation. Food Anal. Methods 10: 1377-1385 (2017)
Smith TJ. Squalene: potential chemopreventive agent. Expert Opin. Inv. Drug. 9: 1841-1848 (2005)
Viola P, Viola M. Virgin olive oil as a fundamental nutritional component and skin protector. Clin. Dermatol. 27: 159-165 (2009)
Zaveri NT. Green tea and its polyphenolic catechins: medicinal uses in cancer and noncancer applications. Life Sci. 78: 2073-2080 (2006)
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This research was financially supported in part by Korean Ministry for Food, Agriculture, Forestry, and Fisheries through iPET (Project Number 113020-3).
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Park, S.Y., Choi, S.J., Park, H.J. et al. Hexane extract of green tea (Camellia sinensis) leaves is an exceptionally rich source of squalene. Food Sci Biotechnol 29, 769–775 (2020). https://doi.org/10.1007/s10068-019-00724-3
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DOI: https://doi.org/10.1007/s10068-019-00724-3