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Differential functional traits underlying the contrasting salt tolerance in Lepidium species

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Abstract

Aims

To explore the mechanisms responsible for salt tolerance in Brassicaceae species, a multifactorial approach was used to clarify the functional traits underlying the differential salt tolerance in two Lepidium species, namely the halophyte L. latifolium and its glycophyte relative, L. sativum.

Methods

Parameters related to photosynthesis, nitrogen assimilation, ion accumulation, water relations and succulence, osmotic adjustment, phenolics metabolism, antioxidative defense, nitric oxide (NO) level and the expression of Na+ antiporter (SOS1 and NHX) were analyzed in plants grown under salt stress in hydroponics.

Results

In addition to significant differences regarding the majority of salt tolerance indicators, a characteristic early boost, 52 h after exposure to salt, was observed in the concentration of H2O2 and NO in the halophyte, which was almost absent in the glycophyte. Following the application of detrended component analysis, discrimination between the glycophyte and halophyte could only be performed via temporal curves in the antioxdative components and NO, and less effectively, by phenolics metabolism.

Conclusion

H2O2 and NO signaling and the adaptive modification of phenolics metabolism play crucial roles in determining the halophytic behavior of L. latifolium. These data may result in new insights concerning the studies on halophytism in the Brassicaceae.

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Abbreviations

APX:

ascorbate peroxidase

MDA:

malondialdehyde

NR:

nitrate reductase

NO:

nitric oxide

POD:

peroxidase

PAL:

phenylalanine ammonia lyase

PPO:

polyphenol oxidase

ROS:

reactive oxygen species

SOD:

superoxide dismutase

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Acknowledgments

This work has been supported by the Center for International Scientific Studies & Collaboration (CISSC), Iran. C. Poschenrieder thanks support from project SAL-CAL-MED from Spanish MICINN BFU2016-75176-R. S. Bahrami-Rad was supported by a postdoctoral fellowship provided by the Research Deputy Office, University of Tabriz, Iran.

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Authors and Affiliations

  1. Department of Plant Science, University of Tabriz, Tabriz, 51666-16471, Iran

    R. Hajiboland, S. Bahrami-Rad & E. Atazadeh

  2. Department of Biology, University of Tabriz, Tabriz, 51666-16471, Iran

    N. Zeinalzade

  3. Halophytes and C4 Plants Research Laboratory, Department of Plant Science, School of Biology, University of Tehran, P.O.Box 14155-6455, Tehran, Iran

    H. Akhani

  4. Plant Physiology Laboratory, Bioscience Faculty, Universidad Autónoma de Barcelona, 08193, Bellaterra, Spain

    C. Poschenrieder

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  1. R. Hajiboland
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Hajiboland, R., Bahrami-Rad, S., Zeinalzade, N. et al. Differential functional traits underlying the contrasting salt tolerance in Lepidium species. Plant Soil 448, 315–334 (2020). https://doi.org/10.1007/s11104-020-04436-0

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