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Structuring Diluted Wheat Matrices: Impact of Heat-Moisture Treatment on Protein Aggregation and Viscoelasticity of Hydrated Composite Flours

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Abstract

The influence of heat-moisture treatment (HMT) and flour hydration (DY) on the restoration of dough viscoelasticity of wheat/non-wheat binary matrices was investigated by applying fundamental and empirical rheological procedures, and the protein structural reorganization was monitored by measuring residual protein solubility in different media, and by assessing the accessibility of thiol groups inside the protein network. Single chestnut (CN), chickpea (CP), millet (MI), and teff (T) flour samples submitted to HMT (15% moisture content, 1 h and 120 °C) were blended with wheat flour at 10% (CN, CP) and 30% (MI, T) of replacement, and binary matrices hydrated at low (L), medium (M), and high (H) DY. Structuring ability of HMT was mainly observed in cereal flour blends (T, MI), where higher elastic moduli and lower loss tangent together with solid-like elastic structure over higher shear stress were observed as compared with treated non-cereal flour blends (CN, CP). Increased flour hydration significantly weakened blends structure, inducing a stepped decrease in dynamic moduli values particularly noticed in cereal blends at highest level of flour hydration, and a shift from elastic-like to viscous-like structure at lower shear stress in non-wheat cereal matrices. The formation of a protein network with reinforced compact structure associated to the presence or formation of intramolecular (CN, CP, T) and intermolecular disulphide bonds (CN, CP, MI, T), water-soluble (CN, MI) and water-insoluble aggregates (CP, T) is feasible to achieve with proteins of non-wheat flours submitted to HMT, particularly in high DY doughs. The lower the amount of free thiols in high molecular weight proteins encompassing high degree of crosslinking, corresponded to thermally treated samples (T, MI) blended at L and M hydration levels. For thermally treated samples, the lower the amount of free thiols in high molecular weight proteins encompassing high degree of crosslinking, corresponded to T and MI binary matrices blended at L and M hydration levels. These samples exhibited a solid-like elastic structure over higher shear stress and showed increased tolerance to stress/strain before losing the structure.

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References

  • Abebe, Y., Bogale, A., Hambidge, K. M., Stoecker, B., Bailey, J. K., & Gibson, R. S. (2007). Phytate, zinc, iron and calcium content of selected raw and prepared foods consumed in rural Sidama, southern Ethiopia, and implications for bioavailability. Journal of Food and Composition Analysis, 20, 161–168.

    CAS  Google Scholar 

  • Adebowale, A.-R., Emmambux, N. M., Beukes, M., & Taylor, J. R. N. (2011). Fractionation and characterization of teff proteins. Journal of Cereal Science, 54, 380–386.

    CAS  Google Scholar 

  • Aitken, A., & Learmonth, M. (2009). Estimation of Disulfide Bonds Using Ellman’s Reagent. In: Walker J.M. (eds), The Protein Protocols Handbook. Springer Protocols Handbooks. Totowa: Humana Press.

  • Alamprese, C., Lametti, S., Rossi, M., & Bergonzi, D. (2005). Role of pasteurisation heat treatments on rheological and protein structural characteristics of fresh egg pasta. European Food Research and Technology, 221, 759–767.

    CAS  Google Scholar 

  • Angioloni, A., & Collar, C. (2012a). High legume-wheat matrices: An alternative to promote bread nutritional value meeting dough viscoelastic restrictions. European Food Research and Technology, 234(2), 273–284.

    CAS  Google Scholar 

  • Angioloni, A., & Collar, C. (2012b). Effects of pressure treatment of hydrated oat, finger millet and sorghum flours on the quality and nutritional properties of composite wheat breads. Journal of Cereal Science, 56, 713–719.

    CAS  Google Scholar 

  • Angioloni, A., & Collar, C. (2013a). Suitability of oat, millet and sorghum in breadmaking. Food and Bioprocess Technology, 6, 1486–1493.

    CAS  Google Scholar 

  • Angioloni, A., & Collar, C. (2013b). Impact of high hydrostatic pressure on protein aggregation and rheological properties of legume batters. Food and Bioprocess Technology, 6, 3576–3584.

    CAS  Google Scholar 

  • Bagdi, A., Balázs, G., Schmidt, J., Szatmári, M., Schoenlechner, R., & Berghofer, E. (2011). Protein characterization and nutrient composition of Hungarian proso millet varieties and the effect of decortication. Acta Alimentaria, 40, 128–141.

    CAS  Google Scholar 

  • Boye, J., Zare, F., & Pletch, A. (2010). Pulse proteins: Processing, characterization, functional properties and applications in food and feed. Food Research International, 43, 414–431.

    CAS  Google Scholar 

  • Cho, S.-H., Choi, Y., & Ha, T.-Y. (2000). In vitro and in vivo effects of proso millet, buckwheat and sorghum on cholesterol metabolism. FASEB Journal, 14, A249.

    Google Scholar 

  • Collar, C. (2003). Significance of viscosity profile of pasted and gelled formulated wheat doughs on bread staling. European Food Research and Technology, 216, 505–513.

    CAS  Google Scholar 

  • Collar, C. (2016). Role of bread on nutrition and health worldwide. In C. M. Rosell, J. Bajerska, & A. F. El Sheikha (Eds.), Bread and its fortification. Nutritional and health benefits (pp. 26–52). Boca Ratón: CRC Press/ Taylor & Francis Group.

    Google Scholar 

  • Collar, C. (2017). Significance of heat moisture treatment conditions on the pasting and gelling behaviour of various starch-rich cereal and pseudocereal flours. Food Science and Technology International, 23(7), 623–636.

    PubMed  CAS  Google Scholar 

  • Collar, C., & Angioloni, A. (2017). High-legume wheat-based matrices: Impact of high pressure on starch hydrolysis and firming kinetics of composite breads. Food and Bioprocess Technology, 10, 1103–1112.

    CAS  Google Scholar 

  • Collar, C., & Armero, E. (2018a). Impact of heat moisture treatment and hydration level on physico-chemical and viscoelastic properties of doughs from wheat-barley composite flours. European Food Research and Technology, 244, 355–366.

    CAS  Google Scholar 

  • Collar, C., & Armero, E. (2018b). Significance of heat moisture treatment and hydration level of flours on the functional and nutritional value of wheat-barley composite breads. Food and Bioprocess Technology, 11, 966–978.

    CAS  Google Scholar 

  • Collar, C., & Armero, E. (2018c). Value-added dough-based fermented mixed matrices by applying heat moisture treatment to non-wheat flours: A functional and nutritional approach of blends. Food and Bioprocess Technology, 11, 1536–1551.

    CAS  Google Scholar 

  • Dall'Asta, C., Cirlini, M., Morini, E., Rinaldi, M., Ganino, T., & Chiavaro, E. (2013). Effect of chestnut flour supplementation on physico-chemical properties and volatiles in bread-making. LWT Food Science and Technology, 53, 233–239.

    CAS  Google Scholar 

  • Delcour, J. A., Joye, I. J., Pareyt, B., Wilderjans, E., Brijs, K., & Lagrain, B. (2012). Wheat gluten functionality as a quality determinant in cereal-based food products. Annual Review of Food Science and Technology, 3, 469–492.

    PubMed  CAS  Google Scholar 

  • FAO/WHO. (2003). Food energy-methods of analysis and conversion factors (p. 77). Rome: FAO Food and Nutrition Paper.

    Google Scholar 

  • Gulati, P., Li, A., Holding, D., Santra, D., Yue Zhang, Y., & Rose, D. J. (2017). Heating reduces proso millet protein digestibility via formation of hydrophobic aggregates. Journal of Agricultural and Food Chemistry, 65(9), 1952–1959.

    PubMed  CAS  Google Scholar 

  • Gunaratne, A., & Hoover, R. (2002). Effect of heat-moisture treatment on the structure and physicochemical properties of tuber and root starches. Carbohydrate Polymers, 49, 425–437.

    CAS  Google Scholar 

  • Hoover, R. (2010). The impact of heat-moisture treatment on molecular structures and properties of starches isolated from different botanical sources. Critical Reviews in Food Science and Nutrition, 50(9), 835–847.

    CAS  Google Scholar 

  • ICC. (1976-1996). Standard Methods of the International Association for Cereal Science and Technology 104/1, 105/2, 110/1, 136, 162. Vienna: The Association.

    Google Scholar 

  • Jacobs, H., & Delcour, J. A. (1998). Hydrothermal modifications of granular starch, with retention of the granular structure: A review. Journal of Agricultural and Food Chemistry, 46, 2895–2905.

    CAS  Google Scholar 

  • Kim, S. K., Choi, H. J., Kang, D. K., & Kim, H. Y. (2012). Starch properties of native proso millet (Panicum miliaceum L.). Agronomy Research, 10, 311–318.

    Google Scholar 

  • Kruger, N. J. (2002). The Bradford method for protein quantitation. In J. M. Walker (Ed.), The protein protocols handbook. Totowa: Humana Press Inc.

    Google Scholar 

  • Lambrecht, M. A., Rombouts, I., Ketelaere, B. D., & Delcour, J. A. (2017). Prediction of heat-induced polymerization of different globular food proteins in mixtures with wheat gluten. Food Chemistry, 221, 1158–1167.

    PubMed  CAS  Google Scholar 

  • Lim, H. S., BeMiller, J. N., & Lim, S.-T. (2003). Effect of dry heating with ionic gum at controlled pH on starch paste viscosity. Cereal Chemistry, 80, 198–202.

    CAS  Google Scholar 

  • Lopes-da-Silva, J. A., Santos, D. M. J., Freitas, A., Brites, C., Gil, A. M., & A. M. (2007). Rheological and nuclear magnetic resonance (NMR) study of the hydration and heating of undeveloped wheat doughs. Journal of Agricultural and Food Chemistry, 55(14), 5636–5644.

    PubMed  CAS  Google Scholar 

  • Mann, J., Schiedt, B., Baumann, A., Conde-Petit, B., & Vilgis, T. A. (2013). Effect of heat treatment on wheat dough rheology and wheat protein solubility. Food Science and Technology International, 20, 341–351.

    Google Scholar 

  • Neill, G., Al-Muhtase, A. H., & Magee, T. R. A. (2012). Optimisation of time/temperature treatment, for heat treated soft wheat flour. Journal of Food Engineering, 113, 422–426.

    Google Scholar 

  • Paciulli, M., Rinaldi, M., Cirlini, M., Scazzina, F., & Chiavaro, E. (2016). Chestnut flour addition in commercial gluten-free bread: A shelf-life study. LWT - Food Science and Technology, 70, 88–95.

    CAS  Google Scholar 

  • Renzetti, S., Dal Bello, F., & Arendt, E. K. (2008). Microstructure, fundamental rheology and baking characteristics of batters and breads from different gluten-free fours treated with a microbial transglutaminase. Journal of Cereal Science, 48, 33–45.

    CAS  Google Scholar 

  • Ronda, F., Pérez-Quirce, S., Angioloni, A., & Collar, C. (2013). Impact of viscous dietary fibres on the viscoelastic behaviour of gluten-free formulated rice doughs: A fundamental and empirical rheological approach. Food Hydrocolloids, 32, 252–262.

    CAS  Google Scholar 

  • Ronda, F., Abebe, W., Pérez-Quirce, S., & Collar, C. (2015). Suitability of tef varieties in mixed wheat flour bread matrices: A physicochemical and nutritional approach. Journal of Cereal Science, 64, 139–146.

    CAS  Google Scholar 

  • Schober, T. J., Bean, S. R., Tilley, M., Smith, B. M., & Ioerger, B. P. (2011). Impact of different isolation procedures on the functionality of zein and kafirin. Journal of Cereal Science, 54, 241–249.

    CAS  Google Scholar 

  • Sun, Q., Gong, M., Li, Y., & Xiong, L. (2014). Effect of dry heat treatment on the physicochemical properties and structure of proso millet flour and starch. Carbohydrate Polymers, 110, 128–134.

    PubMed  CAS  Google Scholar 

  • Vu, T. H., Bean, S., Hsieh, C. F., & Shi, Y.-C. (2017). Changes in protein and starch digestibility in sorghum flour during heat–moisture treatments. Journal of the Science of Food and Agriculture, 97(14), 4770–4779.

    PubMed  CAS  Google Scholar 

  • Wang, X.-Y., Guo, X.-N., & Zhu, K.-X. (2016). Polymerization of wheat gluten and the changes of glutenin macropolymer (GMP) during the production of Chinese steamed bread. Food Chemistry, 201, 275–283.

    PubMed  CAS  Google Scholar 

  • Wang, K.-Q., Luo, S.-Z., Zhong, X.-Y., Cai, J., Jiang, S.-T., & Zheng, Z. (2017). Changes in chemical interactions and protein conformation during heat-induced wheat gluten gel formation. Food Chemistry, 214, 393–399.

    PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This study is financially supported by the Institutions Ministerio de Economía y Competitividad (MINECO) and Federación Europea de Desarrollo Regional (FEDER) through the Projects AGL2015-63849-C2-1-R and AGL2015-63849-C2-2-R, and the Junta de Castilla y León/FEDER VA072P17.

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Authors and Affiliations

  1. Cereals and Cereal-based Products, Food Science Department, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avda. Catedrático Agustín Escardino, 7, 46980, Paterna, Spain

    Concha Collar

  2. Department of Agriculture and Forestry Engineering, Food Technology, College of Agricultural and Forestry Engineering, University of Valladolid, Avda. Madrid, 44, 34004, Palencia, Spain

    Marina Villanueva & Felicidad Ronda

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  1. Concha Collar
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  2. Marina Villanueva
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  3. Felicidad Ronda
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Correspondence to Concha Collar.

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Collar, C., Villanueva, M. & Ronda, F. Structuring Diluted Wheat Matrices: Impact of Heat-Moisture Treatment on Protein Aggregation and Viscoelasticity of Hydrated Composite Flours. Food Bioprocess Technol 13, 475–487 (2020). https://doi.org/10.1007/s11947-020-02406-z

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