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Development of an Isotope Dilution Mass Spectrometry Method for Accurate Determination of Thiamine in Diverse Food Matrices

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Abstract

Many foods contain thiamine as a natural or fortified nutrient. In order to realize accurate measurement of thiamine in various food matrices, analytical methods should be developed on the basis of careful examination of the matrix and the analytes in the matrix. An isotope dilution liquid chromatography–mass spectrometry (ID-LC-MS/MS) method was developed for the analysis of thiamine in various samples such as multivitamin tablets, infant formula, rice, and wheat flour. After using spiked isotope-labeled thiamine HCl (13C4-thiamine HCl) as an internal standard, samples were analyzed by LC/MS in a selected reaction monitoring mode to detect ions at m/z 265 → 122 and m/z 269 → 122 for thiamine and 13C4-thiamine, respectively. To improve the accuracy and precision of this method, the preparation procedure was optimized depending on the chemical form of thiamine present in each sample. Thiamine in multivitamin tablets, infant formula, rice, and wheat flour was analyzed after buffer extraction, acid hydrolysis, and continuous acid and enzyme hydrolyses, respectively. The instrumental analyses were also optimized for individual samples, facilitating excellent repeatability (0.16–0.81%) and reproducibility (0.1–4%). The accuracy of the developed method was verified by comparing the experimental data with the certified values of certified reference materials. Thus, optimization of the preparation procedures and ID-LC-MS/MS method allowed the accurate determination of thiamine in diverse food matrices.

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Funding

This work was supported by the Korea Research Institute of Standards and Science under the project “Establishing measurement standards for organic analysis,” grant 19011050.

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Authors and Affiliations

  1. Division of Chemical and Medical Metrology, Korea Research Institute of Standards and Science, Yuseong, Daejeon, 34113, Korea

    Jayeon Joo, Byungjoo Kim, Sunyoung Lee, Song-Yee Baek & Joonhee Lee

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  1. Jayeon Joo
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  2. Byungjoo Kim
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  3. Sunyoung Lee
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  4. Song-Yee Baek
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  5. Joonhee Lee
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Correspondence to Joonhee Lee.

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Jayeon Joo declares that she has no conflict of interest. Byungjoo Kim declares that he has no conflict of interest. Sunyoung Lee declares that she has no conflict of interest. Song-Yee Baek declares that she has no conflict of interest. Joonhee Lee declares that she has no conflict of interest.

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Joo, J., Kim, B., Lee, S. et al. Development of an Isotope Dilution Mass Spectrometry Method for Accurate Determination of Thiamine in Diverse Food Matrices. Food Anal. Methods 13, 348–357 (2020). https://doi.org/10.1007/s12161-019-01650-0

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  • DOI: https://doi.org/10.1007/s12161-019-01650-0

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