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Micro-computed tomography high resolution evaluation of dimensional and morphological changes of 3 root-end filling materials in simulated physiological conditions

  • Biomaterials Synthesis and Characterization
  • Original Research
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Abstract

The aim of this study was to evaluate volumetric and morphological stability of 3 root-end filling materials in addition to porosity and interface voids, using micro-computed tomography (µCT) in high resolution and a highly accurate approach for image analysis. Following root-end resection and apical preparation, two-rooted maxillary premolars were divided into three groups, according to the filling materials: White MTA Angelus, Biodentine, and IRM. Samples were scanned by µCT at 5 µm after the setting time and at time intervals of 7 and 30 days after immersion in phosphate-buffered saline (PBS). Volumetric and morphological changes besides material porosity and interface voids were evaluated by comparing initial values and those obtained after immersion. Data were analyzed statistically, using ANOVA and t-tests (α = 0.05). All materials showed volumetric stability. Regarding the morphological changes, Biodentine had a significant thickness reduction after storage in PBS when compared with MTA. Biodentine also showed an increase in porosity, as well as in percentage and thickness of voids after 30 days of immersion. In conclusion, µCT in high resolution and an accurate image analysis approach may be used to evaluate morphological changes of endodontic materials. Although Biodentine showed suitable adaptability and lower values of porosity than MTA, after PBS immersion there was a dimensional reduction of this material, besides an increase in porosity and interface voids.

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Acknowledgements

The study was supported by São Paulo State Research Foundation—FAPESP (grants 2016/00321-0, 2017/22481-1, and 2017/19049-0), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil—CAPES (Finance Code 001), National Council for Scientific and Technological Development—CNPq (140215/2015-8 and 308076/2018-4), and Cenpes/Petrobras (ANP2014/00389-8). The authors thank Renato Luiz Carvalho for his assistance with the illustrations.

Author contributions

All authors have contributed significantly and they are all in agreement with the manuscript.

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Authors and Affiliations

  1. Department of Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil

    Fernanda Ferrrari Esteves Torres, Juliane Maria Guerreiro-Tanomaru & Mario Tanomaru-Filho

  2. OMFS IMPATH research group, Department of Imaging & Pathology, Faculty of Medicine, KU Leuven and Oral and Maxillofacial Surgery, University Hospitals Leuven, Leuven, Belgium

    Reinhilde Jacobs & Mostafa EzEldeen

  3. Department of Dental Medicine, Karolinska Institute, Stockholm, Sweden

    Reinhilde Jacobs

  4. Department of Nuclear Energy, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil

    Bernardo Camargo dos Santos

  5. São Carlos Institute of Physics, University of São Paulo, PO Box 369, 13560-970, São Carlos, SP, Brazil

    Éverton Lucas-Oliveira & Tito José Bonagamba

Authors
  1. Fernanda Ferrrari Esteves Torres
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  2. Reinhilde Jacobs
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  3. Mostafa EzEldeen
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  4. Juliane Maria Guerreiro-Tanomaru
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  8. Mario Tanomaru-Filho
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Correspondence to Mario Tanomaru-Filho.

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Torres, F.F.E., Jacobs, R., EzEldeen, M. et al. Micro-computed tomography high resolution evaluation of dimensional and morphological changes of 3 root-end filling materials in simulated physiological conditions. J Mater Sci: Mater Med 31, 14 (2020). https://doi.org/10.1007/s10856-019-6355-2

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