Abstract
Key message
The semidominant EMS-induced mutant w5 affects epicuticular wax deposition and mapped to an approximately 194-kb region on chromosome 7DL.
Abstract
Epicuticular wax is responsible for the glaucous appearance of plants and protects against many biotic and abiotic stresses. In wheat (Triticum aestivum L.), β-diketone is a major component of epicuticular wax in adult plants and contributes to the glaucousness of the aerial organs. In the present study, we identified an ethyl methanesulfonate-induced epicuticular wax-deficient mutant from the elite wheat cultivar Jimai22. Compared to wild-type Jimai22, the mutant lacked β-diketone and failed to form the glaucous coating on all aerial organs. The mutant also had significantly increased in cuticle permeability, based on water loss and chlorophyll efflux. Genetic analysis indicated that the mutant phenotype is controlled by a single, semidominant gene on the long arm of chromosome 7D, which was not allelic to the known wax gene loci W1–W4, and was therefore designated W5. W5 was finely mapped to an ~ 194-kb region (flanked by the molecular markers SSR2 and STARP11) that harbored four annotated genes according to the reference genome of Chinese Spring (RefSeq v1.0). Collectively, these data will broaden the knowledge of the genetic basis underlying epicuticular wax deposition in wheat.
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Acknowledgements
The authors would like to thank Prof. Jianxin Shi (Shanghai Jiao Tong University) for the GC-FID and GC-MS analyses in his laboratory. This work was financially supported by the National Natural Science Foundation of China (31991214 and 91935304) and the National Key Research and Development Program of China (Grant No. 2017YFD0101004).
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ZN conceived the project. The w5 mutant was provided by MZ; LL and ZQ carried out experiments; LC and ZC participated in field trials; QS, HP, YY, ZH, MX, MY, WG, and TW assisted in revising the manuscript; LL analyzed the experimental results; and LL and ZN wrote the manuscript.
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122_2020_3543_MOESM1_ESM.tif
Fig. S1. Phenotypic comparison of Jing411, an F1 plant, and the w5 mutant. a The whole plant appearance of Jing411 (left), an F1 plant (middle), and the w5 mutant (right) at the heading period. Scale bar 10 cm; b Enlarged view of flag leaves, leaf sheaths and spikes of Jing411 (left), an F1 plant (middle), and the w5 mutant (right). Scale bar 5 cm. (TIFF 1410 kb)
122_2020_3543_MOESM2_ESM.tif
Fig. S2. Number of SNP markers with different polymorphisms between the glaucous pools and non-glaucous pools of the F2 population derived from the cross between the w5 and Jing411. a Distribution of the candidate SNPs per chromosome; b The physical positions of the SNPs on chromosome 7D according to the IWGSC RefSeq v1.0. (TIFF 66 kb)
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Fig. S3. Relative expression levels of the three high-confidence genes in the 194.4-kb region in the flag leaf sheath of Jimai22 and the w5 mutant, with TaActin used as the endogenous control. The bars represent standard deviations of the mean levels calculated from three biological replicates. Asterisks indicate that the difference is significant at *P < 0.05. (TIFF 57 kb)
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Fig. S4. Chromosome distribution of wax-related genes in wheat. The red font represents the gene located in this study. (TIFF 403 kb)
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Fig. S5. Sequence comparison of different IWGSC RefSeq versions. a IWGSC RefSeq v.1.0; b IWGSC RefSeq v.2.0. Scale bar 10 kb. Black triangles represent sequences gaps. (TIFF 72 kb)
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Li, L., Qi, Z., Chai, L. et al. The semidominant mutation w5 impairs epicuticular wax deposition in common wheat (Triticum aestivum L.). Theor Appl Genet 133, 1213–1225 (2020). https://doi.org/10.1007/s00122-020-03543-x
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DOI: https://doi.org/10.1007/s00122-020-03543-x