Abstract
In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v−1) using a central composite rotatable design. Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm−2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined. US intensity had a positive effect on enzyme activity. Under optimal conditions (170 s, 180.8 W cm−2, and 25 °C), CMCase activity was 13% higher than that of the control. In the presence of substrate, CMCase activity increased by 33.87% and KM reduced by 23% in relation to that of the control. The theoretical yield of cellulose was 42.08%. Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.
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Funding
This study was supported by CNPq (grant no. 444422/2014-5), FAPESP (grant nos. 04602-3/2016 and 11932-7/2015), and CAPES (finance code 001). We also thank CAPES for the scholarship provided.
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de Carvalho Silvello, M.A., Martínez, J. & Goldbeck, R. Low-frequency Ultrasound with Short Application Time Improves Cellulase Activity and Reducing Sugars Release. Appl Biochem Biotechnol 191, 1042–1055 (2020). https://doi.org/10.1007/s12010-019-03148-1
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DOI: https://doi.org/10.1007/s12010-019-03148-1