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Real-time in situ magnetic measurement of the intracellular biodegradation of iron oxide nanoparticles in a stem cell-spheroid tissue model

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Abstract

The use of magnetic nanoparticles in nanomedicine keeps expanding and, for most applications, the nanoparticles are internalized in cells then left within, bringing the need for accurate, fast, and easy to handle methodologies to assess their behavior in the cellular environment. Herein, a benchtop-size magnetic sensor is introduced to provide real-time precise measurement of nanoparticle magnetism within living cells. The values obtained with the sensor, of cells loaded with different doses of magnetic nanoparticles, are first compared to conventional vibrating sample magnetometry (VSM), and a strong correlation remarkably validates the use of the magnetic sensor as magnetometer to determine the nanoparticle cellular uptake. The sensor is then used to monitor the progressive intracellular degradation of the nanoparticles, over days. Importantly, this real-time in situ measure is performed on a stem cell-spheroid tissue model and can run continuously on a same spheroid, with cells kept alive within. Besides, such continuous magnetic measurement of cell magnetism at the tissue scale does not impact either tissue formation, viability, or stem cell function, including differentiation and extracellular matrix production.

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Acknowledgements

This work was supported by the European Research Council (ERC-2014-CoG project MaTissE #648779). The authors would like to acknowledge the CNanoMat physico-chemical characterizations platform of University Paris 13, and Nicolas Chevalier for his help in controlling the CO2 level in the home-made incubator associated to the magnetic sensor.

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Authors and Affiliations

  1. Laboratoire Matière et Systèmes, Complexes MSC, UMR 7057, CNRS & University of Paris, 75205, Paris Cedex 13, France

    Aurore Van de Walle, Alexandre Fromain, Anouchka Plan Sangnier, Alberto Curcio & Claire Wilhelm

  2. Inserm, U1148, Laboratory for Vascular Translational Science, Sorbonne Paris Nord, Sorbonne Paris Cité, F-93017, Bobigny, France

    Anouchka Plan Sangnier, Laurence Motte & Yoann Lalatonne

  3. Normafin Sàrl, 8 rue Mathilde Girault, 92300, Levallois-Perret, France

    Luc Lenglet

  4. Services de Biochimie et Médecine Nucléaire, Hôpital Avicenne Assistance Publique-Hôpitaux de Paris, F-93009, Bobigny, France

    Yoann Lalatonne

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  1. Aurore Van de Walle
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  2. Alexandre Fromain
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  3. Anouchka Plan Sangnier
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  4. Alberto Curcio
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  5. Luc Lenglet
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Correspondence to Aurore Van de Walle, Laurence Motte, Yoann Lalatonne or Claire Wilhelm.

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Real-time in situ magnetic measurement of the intracellular biodegradation of iron oxide nanoparticles in a stem cell-spheroid tissue model

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Van de Walle, A., Fromain, A., Sangnier, A.P. et al. Real-time in situ magnetic measurement of the intracellular biodegradation of iron oxide nanoparticles in a stem cell-spheroid tissue model. Nano Res. 13, 467–476 (2020). https://doi.org/10.1007/s12274-020-2631-1

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