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The detoxifying effect of Polygonum equisetiforme extracts against dichlorvos (DDVP)-induced oxidative stress and neurotoxicity in the commercial clam Ruditapes decussatus

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Abstract

Effects of Polygonum equisetiforme extracts against dichlorvos were investigated in the commercial clam Ruditapes decussatus. The toxicity of this pesticide was firstly tested in R. decussatus gill and digestive gland tissues using five doses varying from 0.05 to 1 mg/l during 2, 4, and 7 days. Results showed that 0.05 mg/l of DDVP induced oxidative stress and neurotoxicity in R. decussatus after 2 days of exposure. Investigations of the effects of P. equisetiforme extracts in R. decussatus exposed to 0.25 mg/l of DDVP were made in clams receiving three concentrations (0.009, 0.045, and 0.09 g/l) during 4 and 7 days. Antioxidant enzymes SOD and CAT as well as H2O2 content and AChE were quantified by colorimetric method. Four days of exposure to DDVP increased SOD and CAT activities and enhances H2O2 content. AChE levels decreased considerably following DDVP exposure, although a restoration in the enzyme activity was observed with P. equisetiforme extract (E3 = 0.09 g/l). Overall, P. equisetiforme extract at concentration (E1 = 0.009 g/l) prevents oxidative stress caused by DDVP, while 0.09 g/l of P. equisetiforme extract induced an effect similar to that obtained with DDVP alone. Nevertheless, this concentration was found effective for the restoration of the AChE activity.

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Authors and Affiliations

  1. Faculty of Sciences of Bizerte. Laboratory of Environment Bio-monitoring, 7021, Bizerte, Tunisia

    Tahani El Ayari

  2. Faculty of Sciences of Tunis, Biology and Physiology of Aquatic Organisms Laboratory, University of El Manar, Tunis, Tunisia

    Lazhar Mhadhbi

  3. Higher Institute of Applied Biology of Medenine, University of Gabes, Medenine, Tunisia

    Nadia Debara, Marwa Znati & Houcine Dab

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  1. Tahani El Ayari
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  2. Lazhar Mhadhbi
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Correspondence to Tahani El Ayari.

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El Ayari, T., Mhadhbi, L., Debara, N. et al. The detoxifying effect of Polygonum equisetiforme extracts against dichlorvos (DDVP)-induced oxidative stress and neurotoxicity in the commercial clam Ruditapes decussatus. Environ Sci Pollut Res 27, 10449–10458 (2020). https://doi.org/10.1007/s11356-019-07554-0

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