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Association between the transporters ABCA1/G1 and the expression of miR-33a/144 and the carotid intima media thickness in patients with arterial hypertension

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Abstract

ATP-binding cassette membrane transporters (ABC), functions as an outflow facilitator of phospholipids and cellular cholesterol, playing an important role in the development of atherosclerosis and arterial hypertension. ABC’s transporters could post-transcriptionally regulated by miRs. Evaluate the association in the transporters ABCA1 and ABCG1 with the expression of miR-33a and miR-144 and the carotid intima media thickness (cIMT) in patients with essential arterial hypertension. The miR-33a-5p, miR-144-3p and mRNA ABCA1 and ABCG1 expression in monocytes from Mexican hypertensive patients were examined by RT-PCR. The miR-33a and miR-144 expression in monocytes and mRNA ABCA1 and ABCG1 from Mexican hypertensive patients were examined by RT-PCR. This study involved 84 subjects (42 normotensive subjects and 42 patients with essential hypertension). Our study revealed that miR-33a expression (p = 0.001) and miR-144 (p = 0.985) were up-regulated, meanwhile, ABCA1 and ABCG1 transporters were down-regulated (p = 0.007 and p = 0.550 respectively) in hypertensive patients compared with the control group. The trend remains for miR33a/ABCA1 in presence of cIMT. Moreover, an inverse correlation was found with the expression levels of ABCA1 and ABCG1 as well as in HDL-C with miR-33a and miR-144. Our results showed an increase in the expression of miR-33a and miR-144 and an inverse correlation in their target ABCA1 and ABCG1; it may be associated with essential arterial hypertension in patients with cIMT and as consequence for atheromatous plaque.

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Acknowledgements

We thank the participants of this study.

Funding

Funding for this study was provided by Consejo Nacional de Ciencia y Tecnologia (CONACYT), Project Number 273022. Compliance with ethical standards

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Authors and Affiliations

  1. Department of Physiology, Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano No 1, Col. Sección XVI, C.P. 14080, Mexico City, Mexico

    Claudia Huesca-Gómez, Yazmín Estela Torres-Paz, Giovanny Fuentevilla-Álvarez & Ricardo Gamboa

  2. Department of Endocrinology, Instituto Nacional de Cardiología “Ignacio Chávez”, Mexico City, Mexico

    Rocío Martínez-Alvarado & Margarita Torres-Tamayo

  3. Department of Pharmacology, Instituto Nacional de Cardiología “Ignacio Chávez”, Mexico City, Mexico

    Leonardo Del Valle-Mondragón

  4. Department of Immunology, Instituto Nacional de Cardiología “Ignacio Chávez”, Mexico City, Mexico

    Ma. Elena Soto

Authors
  1. Claudia Huesca-Gómez
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  2. Yazmín Estela Torres-Paz
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  3. Rocío Martínez-Alvarado
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  6. Margarita Torres-Tamayo
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  7. Ma. Elena Soto
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  8. Ricardo Gamboa
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Correspondence to Ricardo Gamboa.

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Ethical approval

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (Instituto Nacional de Cardiología, México) and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study.

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Huesca-Gómez, C., Torres-Paz, Y.E., Martínez-Alvarado, R. et al. Association between the transporters ABCA1/G1 and the expression of miR-33a/144 and the carotid intima media thickness in patients with arterial hypertension. Mol Biol Rep 47, 1321–1329 (2020). https://doi.org/10.1007/s11033-019-05229-0

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  • DOI: https://doi.org/10.1007/s11033-019-05229-0

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