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An integrated strategy for rapid on-site screening and determination of prometryn residues in herbs

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Abstract

We produced a prometryn-specific monoclonal antibody and propose a strategy for convenient on-site detection of prometryn residues in herbs for the first time. This strategy has perfect applicability in a complex herbal medicine matrix. The strategy combines a semiquantitative immunochromatographic strip assay with a heterologous indirect competitive ELISA. When there was no matrix interference, the ELISA had a half-maximal inhibitory concentration of 2.6 ng·mL-1 and a limit of detection of 0.2 ng·mL-1. The immunochromatographic strip assay can be completed within 5 min with a visual limit of detection of 1 ng·mL-1. Although the sample matrix had different effects on the sensitivity of the antibody, excellent repeatability and accuracy were achieved. The method was successfully applied for the screening and determination of prometryn residue in multiple complex herb samples for the first time, and the results were in good agreement with those obtained by liquid chromatography–tandem mass spectrometry. The proposed strategy is rapid, of high-throughput, and of low cost, and may be a promising choice for on-site detection of prometryn in different kinds of herbs.

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Acknowledgements

We thank Baomin Wang from the College of Agronomy and Biotechnology, China Agricultural University, China, for providing technical guidance.

Funding

This work was supported by the National Natural Science Foundation of China (no. 81573595 and 81703699), the CAMS Innovation Fund for Medical Sciences (no. 2017-I2M-1-013) and the Biology Key Construction Discipline Fund project.

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Authors and Affiliations

  1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China

    Congmin Liu, Yudan Wang, Lei Zhang, Jia′an Qin, Xiaowen Dou, Yanwei Fu, Qian Li & Meihua Yang

  2. College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, Shandong, China

    Congmin Liu

  3. Jilin Agricultural Science and Technology University, Jilin City, 132101, Jilin, China

    Xue Zhao

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  1. Congmin Liu
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Correspondence to Xue Zhao or Meihua Yang.

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The authors declare that they have no competing interests.

Ethics approval

The use of laboratory animals (mice) was in accordance with the relevant Chinese laws and according to the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College regulations concerning protection of animals used for scientific purposes. The mice used in this study were approved by the Ethics Committee on Experimental Animals and Animal Tests of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College. The review number is SLXD-20170301058.

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Liu, C., Wang, Y., Zhang, L. et al. An integrated strategy for rapid on-site screening and determination of prometryn residues in herbs. Anal Bioanal Chem 412, 621–633 (2020). https://doi.org/10.1007/s00216-019-02224-z

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