Abstract
Main conclusion
A cytochrome P450 and a 2-oxoglutarate-dependent dioxygenase genes responsible, respectively, for the biosyntheses of canonical and non-canonical strigolactones in Lotus japonicus were identified by transcriptome profiling and mutant screening.
Abstract
Strigolactones (SLs) are a group of apocarotenoids with diverse structures that act as phytohormones and rhizosphere signals. The model legume Lotus japonicus produces both canonical and non-canonical SLs, 5-deoxystrigol (5DS) and lotuslactone (LL), respectively, through oxidation of a common intermediate carlactone by the cytochrome P450 (CYP) enzyme MAX1. However, the pathways downstream of MAX1 and the branching point in the biosyntheses of 5DS and LL have not been elucidated. Here, we identified a CYP and a 2-oxoglutarate-dependent dioxygenase (2OGD) genes responsible, respectively, for the formation of Lotus SLs by transcriptome profiling using RNA-seq and screening of SL-deficient mutants from the Lotus retrotransposon 1 (LORE1) insertion mutant resource. The CYP and 2OGD genes were named DSD and LLD, respectively, after 5DS or LL defective phenotype of the mutants. The involvements of the genes in Lotus SL biosyntheses were confirmed by restoration of the mutant phenotype using Agrobacterium rhizogenes-mediated transformation to generate transgenic roots expressing the coding sequence. The transcript levels of DSD and LLD in roots as well as the levels of 5DS and LL in root exudates were reduced by phosphate fertilization and gibberellin treatment. This study can provide the opportunity to investigate how and why plants produce the two classes of SLs.
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Abbreviations
- CLA:
-
Carlactonoic acid
- CYP:
-
Cytochrome P450
- DSD:
-
5-Deoxystrigol defective
- 5DS:
-
5-Deoxystrigol
- LL:
-
Lotuslactone
- LLD:
-
Lotuslactone defective
- LORE1:
-
Lotus retrotransposon 1
- MAX1:
-
MORE AXILLARY GROWTH 1
- 2OGD:
-
2-Oxoglutarate-dependent dioxygenase
- SLs:
-
Strigolactones
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Acknowledgements
We thank Masayoshi Kawaguchi (National Institute for Basic Biology) for providing Agrobacterium rhizogenes AR1193, and Hiromu Kameoka (Osaka Prefecture University) for discussion on RNA-Seq analysis. This work was supported by the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry, and Grant-in-Aid for JSPS Fellows [Grant Number 17J05519].
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Mori, N., Nomura, T. & Akiyama, K. Identification of two oxygenase genes involved in the respective biosynthetic pathways of canonical and non-canonical strigolactones in Lotus japonicus. Planta 251, 40 (2020). https://doi.org/10.1007/s00425-019-03332-x
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DOI: https://doi.org/10.1007/s00425-019-03332-x